Please use this identifier to cite or link to this item:
10.1099/jgv.0.000692
Title: | Possible chromosomal and germline integration of human herpesvirus 7 |
Authors: | Prusty, Bhupesh K. Gulve, Nitish Rasa, Santa Murovska, Modra Hernandez, Pilar Collado Ablashi, Dharam V. Institute of Microbiology and Virology |
Keywords: | Chromosomal integration;ciHHV-6;HHV-6;HHV-7;1.6 Biological sciences;3.3 Health sciences;1.1. Scientific article indexed in Web of Science and/or Scopus database;Virology |
Issue Date: | Feb-2017 |
Citation: | Prusty , B K , Gulve , N , Rasa , S , Murovska , M , Hernandez , P C & Ablashi , D V 2017 , ' Possible chromosomal and germline integration of human herpesvirus 7 ' , Journal of General Virology , vol. 98 , no. 2 , pp. 266-274 . https://doi.org/10.1099/jgv.0.000692 |
Abstract: | Human herpesvirus 7 (HHV-7) is a betaherpesvirus, and is phylogenetically related to both HHV-6A and HHV-6B. The presence of telomeric repeat sequences at both ends of its genome should make it equally likely to integrate into the human telomere as HHV-6. However, numerous studies have failed to detect germline integration of HHV-7, suggesting an important difference between the HHV-6A/-6B and HHV-7 genomes. In search of possible germline integrated HHV-7, we developed a sensitive and quantitative real-time PCR assay and discovered that primers designed against some parts of the HHV-7 genome can frequently miss HHV-7 positive clinical samples even though they work efficiently in cell-culture-derived HHV-7 positive materials. Using a primer pair against the U90 ORF of HHV-7, we identified a possible case of germline integration of HHV-7 with one copy of viral genome per cell in both peripheral blood cells and hair follicles. Chromosomal integration of HHV-7 in these individuals was confirmed by fluorescence in situ hybridization analysis. Germline integration of HHV-7 was further confirmed by detection of ~2.6 copies of HHV-7 in the hair follicles of one of the parents. Our results shed light on the complex nature of the HHV-7 genome in human-derived materials in comparison to cell-culture-derived materials and show the need for stringent criteria in the selection of primers for epidemiological HHV-7 studies. |
Description: | Publisher Copyright: © 2017 The Authors. Copyright: Copyright 2017 Elsevier B.V., All rights reserved. |
DOI: | 10.1099/jgv.0.000692 |
ISSN: | 0022-1317 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
Files in This Item:
File | Size | Format | |
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Possible_chromosomal_and_germline_integration.pdf | 3.03 MB | Adobe PDF | View/Open |
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