Please use this identifier to cite or link to this item:
10.1099/jgv.0.000692
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DC Field | Value | Language |
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dc.contributor.author | Prusty, Bhupesh K. | - |
dc.contributor.author | Gulve, Nitish | - |
dc.contributor.author | Rasa, Santa | - |
dc.contributor.author | Murovska, Modra | - |
dc.contributor.author | Hernandez, Pilar Collado | - |
dc.contributor.author | Ablashi, Dharam V. | - |
dc.date.accessioned | 2021-04-20T11:00:01Z | - |
dc.date.available | 2021-04-20T11:00:01Z | - |
dc.date.issued | 2017-02 | - |
dc.identifier.citation | Prusty , B K , Gulve , N , Rasa , S , Murovska , M , Hernandez , P C & Ablashi , D V 2017 , ' Possible chromosomal and germline integration of human herpesvirus 7 ' , Journal of General Virology , vol. 98 , no. 2 , pp. 266-274 . https://doi.org/10.1099/jgv.0.000692 | - |
dc.identifier.issn | 0022-1317 | - |
dc.identifier.uri | https://dspace.rsu.lv/jspui/handle/123456789/3861 | - |
dc.description | Publisher Copyright: © 2017 The Authors. Copyright: Copyright 2017 Elsevier B.V., All rights reserved. | - |
dc.description.abstract | Human herpesvirus 7 (HHV-7) is a betaherpesvirus, and is phylogenetically related to both HHV-6A and HHV-6B. The presence of telomeric repeat sequences at both ends of its genome should make it equally likely to integrate into the human telomere as HHV-6. However, numerous studies have failed to detect germline integration of HHV-7, suggesting an important difference between the HHV-6A/-6B and HHV-7 genomes. In search of possible germline integrated HHV-7, we developed a sensitive and quantitative real-time PCR assay and discovered that primers designed against some parts of the HHV-7 genome can frequently miss HHV-7 positive clinical samples even though they work efficiently in cell-culture-derived HHV-7 positive materials. Using a primer pair against the U90 ORF of HHV-7, we identified a possible case of germline integration of HHV-7 with one copy of viral genome per cell in both peripheral blood cells and hair follicles. Chromosomal integration of HHV-7 in these individuals was confirmed by fluorescence in situ hybridization analysis. Germline integration of HHV-7 was further confirmed by detection of ~2.6 copies of HHV-7 in the hair follicles of one of the parents. Our results shed light on the complex nature of the HHV-7 genome in human-derived materials in comparison to cell-culture-derived materials and show the need for stringent criteria in the selection of primers for epidemiological HHV-7 studies. | en |
dc.format.extent | 9 | - |
dc.format.extent | 3100312 | - |
dc.language.iso | eng | - |
dc.relation.ispartof | Journal of General Virology | - |
dc.rights | info:eu-repo/semantics/openAccess | - |
dc.subject | Chromosomal integration | - |
dc.subject | ciHHV-6 | - |
dc.subject | HHV-6 | - |
dc.subject | HHV-7 | - |
dc.subject | 1.6 Biological sciences | - |
dc.subject | 3.3 Health sciences | - |
dc.subject | 1.1. Scientific article indexed in Web of Science and/or Scopus database | - |
dc.subject | Virology | - |
dc.title | Possible chromosomal and germline integration of human herpesvirus 7 | en |
dc.type | /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article | - |
dc.identifier.doi | 10.1099/jgv.0.000692 | - |
dc.contributor.institution | Institute of Microbiology and Virology | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85015703561&partnerID=8YFLogxK | - |
dc.description.status | Peer reviewed | - |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
Files in This Item:
File | Size | Format | |
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Possible_chromosomal_and_germline_integration.pdf | 3.03 MB | Adobe PDF | View/Open |
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