Please use this identifier to cite or link to this item:
10.3390/medicina55120774
Title: | Presence of B19V in patients with thyroid gland disorders |
Authors: | Gravelsina, Sabine Nora-Krukle, Zaiga Svirskis, Simons Cunskis, Egils Murovska, Modra Institute of Microbiology and Virology |
Keywords: | B19V;Thyroid gland diseases;3.2 Clinical medicine;3.3 Health sciences;1.1. Scientific article indexed in Web of Science and/or Scopus database;General Medicine |
Issue Date: | Dec-2019 |
Citation: | Gravelsina , S , Nora-Krukle , Z , Svirskis , S , Cunskis , E & Murovska , M 2019 , ' Presence of B19V in patients with thyroid gland disorders ' , Medicina (Lithuania) , vol. 55 , no. 12 , 774 . https://doi.org/10.3390/medicina55120774 |
Abstract: | Background and Objectives: Viral infections are frequently cited as a major environmental factor implicated in thyroid gland diseases. This work aimed to estimate the presence of B19V infection in patients with thyroid gland disorders. Materials and Methods: Thyroid gland tissue and blood samples of 50 patients with autoimmune thyroid gland diseases (AITDs), 76 patients with non-autoimmune thyroid gland diseases (non-AITDs), and 35 deceased subjects whose histories did not show any autoimmune or thyroid diseases (control group) were enrolled in the study. Virus-specific IgM and IgG were detected using ELISA, and the presence and viral load of B19V in the tissue and blood were detected using PCRs. Results: B19V IgG antibodies were detected in 35/50 AITDs patients and in 51/76 non-AITDs patients, and B19V IgM antibodies were detected in 1/50 patients with AITDs and in none of the 76 patients with non-AITDs. The B19V NS sequence was found in the tissue DNA of 10/50 patients with AITDs, in 30/76 with non-AITDs, and in 1/35 control group individuals. The median B19V load in the tissue of patients with AITDs and non-AITDs was 423.00 copies/µg DNA (IQR: 22.50–756.8) and 43.00 copies/µg DNA (IQR: 11.50–826.5), respectively. The viral load in one of the 35 nPCR B19V-positive thyroid tissue samples from the deceased subjects was 13.82 copies/µg DNA. The viral load in the tissue of patients with AITDs was higher than in whole blood, which possibly indicates B19V persistency in thyrocytes (p = 0.0076). Conclusion: The fact that the genoprevalence of B19V NS was significantly higher in patients with non-AITDs compared to the control group and in the thyroid gland tissue of patients with AITDs, and that the non-AITDs viral load was higher than in tissue derived from the control group individuals, suggest the possibility that B19V infection could be involved in the development of thyroid gland diseases. |
Description: | Funding Information: Funding: This research was supported by the research cooperation project “Beta-herpes viruses (HHV-6, HHV-7) and parvovirus B19 infections as possible risk factors for the development of autoimmune thyroid disease” (SZA cooperation project; grant number 10.0029.4) and by the National Research Programme “Biomedicine for the Public Health” (BIOMEDICINE). Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2019 Elsevier B.V., All rights reserved. |
DOI: | 10.3390/medicina55120774 |
ISSN: | 1010-660X |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
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Presence_of_B19V_in_Patients_with_Thyroid_Gland_Disorders.pdf | 1.06 MB | Adobe PDF | View/Open |
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