Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism
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Date
2017-08-01
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Abstract
The non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism’s potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.
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Funding Information: The research was supported by ERDF project Nr. 2DP/2.1.1.1.0/14/APIA/VIAA/043. Publisher Copyright: © 2017, Society for Industrial Microbiology and Biotechnology.
Keywords
Central metabolism, Essentiality analysis, Kluyveromyces marxianus, Metabolic engineering, Modelling, 3.4 Medical biotechnology, 1.1. Scientific article indexed in Web of Science and/or Scopus database, Biotechnology, Bioengineering, Applied Microbiology and Biotechnology
Citation
Pentjušs, A, Stalidzāns, E, Liepiņš, J, Kokina, A, Martinova, J, Zikmanis, P, Mozga, I, Ščerbaka, R, Hartman, H, Poolman, M G, Fell, D A & Vīgants, A 2017, 'Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism', Journal of Industrial Microbiology and Biotechnology, vol. 44, no. 8, pp. 1177-1190. https://doi.org/10.1007/s10295-017-1946-8