Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism

Simple item page
dc.contributor.authorPentjušs, Agris
dc.contributor.authorStalidzāns, Egils
dc.contributor.authorLiepiņš, Jānis
dc.contributor.authorKokina, Agnese
dc.contributor.authorMartinova, Jekaterina
dc.contributor.authorZikmanis, Pēteris
dc.contributor.authorMozga, Ivars
dc.contributor.authorŠčerbaka, Rita
dc.contributor.authorHartman, H.
dc.contributor.authorPoolman, M. G.
dc.contributor.authorFell, D. A.
dc.contributor.authorVīgants, Armands
dc.date.accessioned2022-11-07T09:00:02Z
dc.date.available2022-11-07T09:00:02Z
dc.date.issued2017-08-01
dc.descriptionFunding Information: The research was supported by ERDF project Nr. 2DP/2.1.1.1.0/14/APIA/VIAA/043. Publisher Copyright: © 2017, Society for Industrial Microbiology and Biotechnology.
dc.description.abstractThe non-conventional yeast Kluyveromyces marxianus is an emerging industrial producer for many biotechnological processes. Here, we show the application of a biomass-linked stoichiometric model of central metabolism that is experimentally validated, and mass and charge balanced for assessing the carbon conversion efficiency of wild type and modified K. marxianus. Pairs of substrates (lactose, glucose, inulin, xylose) and products (ethanol, acetate, lactate, glycerol, ethyl acetate, succinate, glutamate, phenylethanol and phenylalanine) are examined by various modelling and optimisation methods. Our model reveals the organism’s potential for industrial application and metabolic engineering. Modelling results imply that the aeration regime can be used as a tool to optimise product yield and flux distribution in K. marxianus. Also rebalancing NADH and NADPH utilisation can be used to improve the efficiency of substrate conversion. Xylose is identified as a biotechnologically promising substrate for K. marxianus.en
dc.description.statusPeer reviewed
dc.format.extent14
dc.format.extent1383645
dc.identifier.citationPentjušs, A, Stalidzāns, E, Liepiņš, J, Kokina, A, Martinova, J, Zikmanis, P, Mozga, I, Ščerbaka, R, Hartman, H, Poolman, M G, Fell, D A & Vīgants, A 2017, 'Model-based biotechnological potential analysis of Kluyveromyces marxianus central metabolism', Journal of Industrial Microbiology and Biotechnology, vol. 44, no. 8, pp. 1177-1190. https://doi.org/10.1007/s10295-017-1946-8
dc.identifier.doi10.1007/s10295-017-1946-8
dc.identifier.issn1367-5435
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/9748
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85018670164&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of Industrial Microbiology and Biotechnology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCentral metabolism
dc.subjectEssentiality analysis
dc.subjectKluyveromyces marxianus
dc.subjectMetabolic engineering
dc.subjectModelling
dc.subject3.4 Medical biotechnology
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectBiotechnology
dc.subjectBioengineering
dc.subjectApplied Microbiology and Biotechnology
dc.titleModel-based biotechnological potential analysis of Kluyveromyces marxianus central metabolismen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
Model_based_biotechnological_potential_analysis.pdf
Size:
1.32 MB
Format:
Adobe Portable Document Format
Download

Collections

Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure