Please use this identifier to cite or link to this item:
10.1155/2021/6265553
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DC Field | Value | Language |
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dc.contributor.author | Azzolini, Claudio | - |
dc.contributor.author | Donati, Simone | - |
dc.contributor.author | Micheloni, Giovanni | - |
dc.contributor.author | Moretti, Vittoria | - |
dc.contributor.author | Valli, Roberto | - |
dc.contributor.author | Acquati, Francesco | - |
dc.contributor.author | Costantino, Lucy | - |
dc.contributor.author | Ferrara, Fulvio | - |
dc.contributor.author | Borroni, Davide | - |
dc.contributor.author | Premi, Elias | - |
dc.contributor.author | Testa, Francesco | - |
dc.contributor.author | Simonelli, Francesca | - |
dc.contributor.author | Porta, Giovanni | - |
dc.date.accessioned | 2022-02-23T10:40:02Z | - |
dc.date.available | 2022-02-23T10:40:02Z | - |
dc.date.issued | 2021 | - |
dc.identifier.citation | Azzolini , C , Donati , S , Micheloni , G , Moretti , V , Valli , R , Acquati , F , Costantino , L , Ferrara , F , Borroni , D , Premi , E , Testa , F , Simonelli , F & Porta , G 2021 , ' Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia ' , Journal of Ophthalmology , vol. 2021 , 6265553 . https://doi.org/10.1155/2021/6265553 | - |
dc.identifier.issn | 2090-004X | - |
dc.identifier.uri | https://dspace.rsu.lv/jspui/handle/123456789/7641 | - |
dc.description | Publisher Copyright: Copyright © 2021 Claudio Azzolini et al. | - |
dc.description.abstract | Introduction. Müller glial cells typically activate to react to hypoxic tissue damage in several retinal diseases. We evaluated the in vitro response to a hypoxia-mimicking stimulus on the expression of a set of genes, known to contribute to eye morphogenesis and cell differentiation. Materials and Methods. A MIO-M1 Müller cell line was cultured in a hypoxia-mimicking environment by the addition of cobalt chloride to the culture medium, followed by a recovery time in which we mimic restoration from the hypoxic insult. The HIF-1α protein and VEGF-A gene expression were quantified to verify the induction of a hypoxia-like state. Results. Among the genes under study, we did not observe any difference in the expression levels of Otx1 and Otx2 during treatment; conversely, Otx1 was overexpressed during recovery steps. The VEGF-A gene was strongly upregulated at both the CoCl2 and recovery time points. The transactivated isoform (TA) of the TP73 gene showed an overexpression in long-term exposure to the hypoxic stimulus with a further increase after recovery. Discussion. Our molecular analysis is able to describe the activation of a set of genes, never before described, that can drive the response to a hypoxia-like status. The improved comprehension of these cellular events will be useful for designing new therapeutical approaches for retinal pathologies. | en |
dc.format.extent | 3117089 | - |
dc.language.iso | eng | - |
dc.relation.ispartof | Journal of Ophthalmology | - |
dc.rights | info:eu-repo/semantics/openAccess | - |
dc.subject | 3.1 Basic medicine | - |
dc.subject | 3.2 Clinical medicine | - |
dc.subject | 1.1. Scientific article indexed in Web of Science and/or Scopus database | - |
dc.subject | Ophthalmology | - |
dc.title | Expression of Otx Genes in Müller Cells Using an In Vitro Experimental Model of Retinal Hypoxia | en |
dc.type | /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article | - |
dc.identifier.doi | 10.1155/2021/6265553 | - |
dc.contributor.institution | Department of Doctoral Studies | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85122958358&partnerID=8YFLogxK | - |
dc.description.status | Peer reviewed | - |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
Files in This Item:
File | Size | Format | |
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Expression_of_Otx_Genes.pdf | 3.04 MB | Adobe PDF | View/Open |
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