Please use this identifier to cite or link to this item:
10.1074/jbc.M115.670471
Title: | Structure and function of CutC choline lyase from human microbiota bacterium Klebsiella pneumoniae |
Authors: | Kalnins, Gints Kuka, Janis Grinberga, Solveiga Makrecka-Kuka, Marina Liepinsh, Edgars Dambrova, Maija Tars, Kaspars |
Keywords: | 1.6 Biological sciences;3.1 Basic medicine;1.1. Scientific article indexed in Web of Science and/or Scopus database;Biochemistry;Molecular Biology;Cell Biology;SDG 3 - Good Health and Well-being |
Issue Date: | 28-Aug-2015 |
Citation: | Kalnins , G , Kuka , J , Grinberga , S , Makrecka-Kuka , M , Liepinsh , E , Dambrova , M & Tars , K 2015 , ' Structure and function of CutC choline lyase from human microbiota bacterium Klebsiella pneumoniae ' , Journal of Biological Chemistry , vol. 290 , no. 35 , pp. 21732-21740 . https://doi.org/10.1074/jbc.M115.670471 |
Abstract: | CutC choline trimethylamine-lyase is an anaerobic bacterial glycyl radical enzyme (GRE) that cleaves choline to produce trimethylamine (TMA) and acetaldehyde. In humans, TMA is produced exclusively by the intestinal microbiota, and its metabolite, trimethylamine oxide, has been associated with a higher risk of cardiovascular diseases. Therefore, information about the three-dimensional structures of TMA-producing enzymes is important for microbiota-targeted drug discovery. We have cloned, expressed, and purified the CutC GRE and the activating enzyme CutD from Klebsiella pneumoniae, a representative of the human microbiota. We have determined the first crystal structures of both the choline-bound and choline-free forms of CutC and have discovered that binding of choline at the ligand-binding site triggers conformational changes in the enzyme structure, a feature that has not been observed for any other characterized GRE. |
Description: | Publisher Copyright: © 2015 by The American Society for Biochemistry and Molecular Biology, Inc. |
DOI: | 10.1074/jbc.M115.670471 |
ISSN: | 0021-9258 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
Files in This Item:
File | Size | Format | |
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Structure_and_Function_of_CutC_Choline_Lyase.pdf | 2.8 MB | Adobe PDF | View/Open |
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