Structure and function of CutC choline lyase from human microbiota bacterium Klebsiella pneumoniae

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dc.contributor.authorKalnins, Gints
dc.contributor.authorKuka, Janis
dc.contributor.authorGrinberga, Solveiga
dc.contributor.authorMakrecka-Kuka, Marina
dc.contributor.authorLiepinsh, Edgars
dc.contributor.authorDambrova, Maija
dc.contributor.authorTars, Kaspars
dc.date.accessioned2022-01-10T09:40:02Z
dc.date.available2022-01-10T09:40:02Z
dc.date.issued2015-08-28
dc.descriptionPublisher Copyright: © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.
dc.description.abstractCutC choline trimethylamine-lyase is an anaerobic bacterial glycyl radical enzyme (GRE) that cleaves choline to produce trimethylamine (TMA) and acetaldehyde. In humans, TMA is produced exclusively by the intestinal microbiota, and its metabolite, trimethylamine oxide, has been associated with a higher risk of cardiovascular diseases. Therefore, information about the three-dimensional structures of TMA-producing enzymes is important for microbiota-targeted drug discovery. We have cloned, expressed, and purified the CutC GRE and the activating enzyme CutD from Klebsiella pneumoniae, a representative of the human microbiota. We have determined the first crystal structures of both the choline-bound and choline-free forms of CutC and have discovered that binding of choline at the ligand-binding site triggers conformational changes in the enzyme structure, a feature that has not been observed for any other characterized GRE.en
dc.description.statusPeer reviewed
dc.format.extent9
dc.format.extent2871393
dc.identifier.citationKalnins, G, Kuka, J, Grinberga, S, Makrecka-Kuka, M, Liepinsh, E, Dambrova, M & Tars, K 2015, 'Structure and function of CutC choline lyase from human microbiota bacterium Klebsiella pneumoniae', Journal of Biological Chemistry, vol. 290, no. 35, pp. 21732-21740. https://doi.org/10.1074/jbc.M115.670471
dc.identifier.doi10.1074/jbc.M115.670471
dc.identifier.issn0021-9258
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/7180
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84940707248&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofJournal of Biological Chemistry
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subject1.6 Biological sciences
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectCell Biology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleStructure and function of CutC choline lyase from human microbiota bacterium Klebsiella pneumoniaeen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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