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DOI: 10.9734/BJMMR/2015/19676
Title: | Basic Fibroblast Growth Factor (bFGF), Fibroblast Growth Factor Receptor 1 (FGFR1), Transforming Growth Factor Beta (TGF-β) and Chromogranin A (CgA) Appearance in Congenital Intra-abdominal Adhesions in Children under One Year of Age |
Authors: | Augule, Anna Pilmane, Māra Ābola, Zane Volrāts, Olafs Institute of Anatomy and Anthropology Department of Paediatric Surgery |
Keywords: | children;adhesions;growth factors;immunohistochemistry;3.1 Basic medicine;3.2 Clinical medicine;1.4. Reviewed scientific article published in Latvia or abroad in a scientific journal with an editorial board (including university editions) |
Issue Date: | 9-Sep-2015 |
Citation: | Augule , A , Pilmane , M , Ābola , Z & Volrāts , O 2015 , ' Basic Fibroblast Growth Factor (bFGF), Fibroblast Growth Factor Receptor 1 (FGFR1), Transforming Growth Factor Beta (TGF-β) and Chromogranin A (CgA) Appearance in Congenital Intra-abdominal Adhesions in Children under One Year of Age ' , British Journal of Medicine and Medical Research , vol. 10 , no. 11 , 19676 , pp. 1-9 . https://doi.org/DOI: 10.9734/BJMMR/2015/19676 |
Abstract: | Aim: Of this work was to determine dispersion of TGF-b, fibrosis modulating factor (bFGF and FGR1) and granule marker chromogranin A in case of intra-abdominal adhesion which could be essential factors in disease pathogenesis. Thus than could be used as possible biomarker. Study Design: Observational study. Place of Study: Institute of Anatomy and Anthropology and Department of Children Surgery, Rīga Stradiņš University. Materials and Methods: The specimens used for research were obtained from 50 patients aged 1 to 292 days. They underwent abdominal surgery due to obstructive gut malrotation and several additional pathologies. Tissues were processed for bFGF, FGFR1, TGF-β and CgA by means of biotin-streptavidin immunohistochemistry. Results: In adhesion tissue bFGF positive connective tissue cells varied from a few to an abundant amount, but in 15 specimens no positive structure was observed. Few connective tissue fibers and moderate to numerous fibroblasts and macrophages contained FGFR1. A moderate number of TGF-β positive connective tissue fibers were observed. Some specimens also contained positive fibroblasts, macrophages and endotheliocytes. Few connective tissue cells contained CgA. A moderate correlation was observed between bFGF and FGFR1 (Spearman’s rank correlation coefficient = .500, P < .001) as well as between bFGF and CgA (Spearman’s rank correlation coefficient = .311, P = .03). Conclusions: The connection between the less distinct bFGF and more prominent FGFR1 proves the compensatory stimulation of receptors as a response on the lack of the same factor in case of adhesion disease. Persisting appearance of TGF-β positive structures in congenital adhesions indicates the continuing growth/regeneration potential of loose connective tissue. Positive CgA structures indicate the involvement of the neuroendocrine system in case of adhesion disease. |
DOI: | DOI: 10.9734/BJMMR/2015/19676 |
ISSN: | 2231-0614 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
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