Please use this identifier to cite or link to this item:
10.3390/jpm11111135
Title: | Evaluating the Expression of Candidate Homeobox Genes and Their Role in Local-Site Inflammation in Mucosal Tissue Obtained from Children with Non-Syndromic Cleft Lip and Palate |
Authors: | Jain, Nityanand Pilmane, Māra Department of Morphology Institute of Anatomy and Anthropology |
Keywords: | cleft lip and palate;inflammation;immunohistochemistry;in-situ hybridization;HOXB3;DLX4;MSX2;PTX3;3.1 Basic medicine;3.2 Clinical medicine;1.1. Scientific article indexed in Web of Science and/or Scopus database |
Issue Date: | 2-Nov-2021 |
Citation: | Jain , N & Pilmane , M 2021 , ' Evaluating the Expression of Candidate Homeobox Genes and Their Role in Local-Site Inflammation in Mucosal Tissue Obtained from Children with Non-Syndromic Cleft Lip and Palate ' , Journal of Personalized Medicine , vol. 11 , no. 11 , 1135 . https://doi.org/10.3390/jpm11111135 , https://doi.org/10.3390/jpm11111135 |
Abstract: | Craniofacial development including palatogenesis is a complex process which requires an orchestrated and spatiotemporal expression of various genes and factors for proper embryogenesis and organogenesis. One such group of genes essential for craniofacial development is the homeobox genes, transcriptional factors that are commonly associated with congenital abnormalities. Amongst these genes, DLX4, HOXB3, and MSX2 have been recently shown to be involved in the etiology of non-syndromic cleft lip and palate. Hence, we investigated the gene and protein expression of these genes in normal and cleft affected mucosal tissue obtained from 22 children, along with analyzing their role in promoting local-site inflammation using NF-κB. Additionally, we investigated the role of PTX3, which plays a critical role in tissue remodeling and wound repair. We found a residual gene and protein expression of DLX4 in cleft mucosa, although no differences in gene expression levels of HOXB3 and MSX2 were noted. However, a significant increase in protein expression for these genes was noted in the cleft mucosa (p < 0.05), indicating increased cellular proliferation. This was coupled with a significant increase in NF-κB protein expression in cleft mucosa (p < 0.05), highlighting the role of these genes in promotion of pro-inflammatory environment. Finally, no differences in gene expression of PTX3 were noted. |
Description: | Funding Information: Funding: The present study was funded by Riga Stradinš University (RSU). Funding Information: The present study was funded by Riga Stradin? University (RSU).We would like to extend our gratitude to Ilze Akota, Department of Oral & Maxillofacial Surgery, Faculty of Dentistry, RSU for providing the patient material. We would also like to acknowledge the parents of the patients for consenting to participate in the present study. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
DOI: | 10.3390/jpm11111135 |
ISSN: | 2075-4426 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
Files in This Item:
File | Size | Format | |
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Evaluating_the_Expression_of_Candidate.pdf | 26.79 MB | Adobe PDF | View/Open |
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