Please use this identifier to cite or link to this item:
10.1105/tpc.106.043869
Title: | Increased level of polyploidy1, a conserved repressor of CYCLINA2 transcription, controls endoreduplication in Arabidopsis |
Authors: | Yoshizumi, Takeshi Tsumoto, Yuko Takiguchi, Tomoko Nagata, Noriko Yamamoto, Yoshiharu Y. Kawashima, Mika Ichikawa, Takanari Nakazawa, Miki Yamamoto, Naoki Matsui, Minami |
Keywords: | 1.6 Biological sciences;1.1. Scientific article indexed in Web of Science and/or Scopus database;Plant Science;Cell Biology |
Issue Date: | Oct-2006 |
Citation: | Yoshizumi , T , Tsumoto , Y , Takiguchi , T , Nagata , N , Yamamoto , Y Y , Kawashima , M , Ichikawa , T , Nakazawa , M , Yamamoto , N & Matsui , M 2006 , ' Increased level of polyploidy1, a conserved repressor of CYCLINA2 transcription, controls endoreduplication in Arabidopsis ' , Plant Cell , vol. 18 , no. 10 , pp. 2452-2468 . https://doi.org/10.1105/tpc.106.043869 |
Abstract: | Endoreduplication is a type of cell cycle in which DNA replication continues without cell division. We have isolated several dominant mutants from Arabidopsis thaliana activation tagging lines by flow cytometry. One of the mutants, increased level of polyploidy1-1D (Hp1-1D), showed increased polyploidy in both light- and dark-grown hypocotyls. The corresponding gene of ilp1-1D encodes a protein homologous to the C-terminal region of mammalian GC binding factor. We demonstrate that this protein functions as a transcriptional repressor in vivo. The expression of all members of the CYCLINA2 (CYCA2) family was reduced in an ILP1 overexpressing line, and the mouse (Mus musculus) homolog of ILP1 repressed cyclin A2 expression in mouse NIH3T3 cells. T-DNA insertion mutants of ILP1 showed reduced polyploidy and upregulated all CYCA2 expression. Furthermore, loss of CYCA2;1 expression induces an increase in polyploidy in Arabidopsis. We demonstrate that this protein regulates endoreduplication through control of CYCA2 expression in Arabidopsis. |
DOI: | 10.1105/tpc.106.043869 |
ISSN: | 1040-4651 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
Files in This Item:
File | Size | Format | |
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INCREASED_LEVEL_OF_POLYPLOIDY1.pdf | 2.21 MB | Adobe PDF | View/Open |
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