Please use this identifier to cite or link to this item: 10.2478/prolas-2020-0028
Title: Appearance and distribution of tissue remodellation factors in the hard tissue of patients affected by cleft lip palate
Authors: Buile, Dace
Pilmane, Māra
Akota, Ilze
Institute of Anatomy and Anthropology
Rīga Stradiņš University
Keywords: Growth factors;Human;3.2 Clinical medicine;1.1. Scientific article indexed in Web of Science and/or Scopus database;General;SDG 3 - Good Health and Well-being
Issue Date: 1-Jun-2020
Citation: Buile , D , Pilmane , M & Akota , I 2020 , ' Appearance and distribution of tissue remodellation factors in the hard tissue of patients affected by cleft lip palate ' , Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences , vol. 74 , no. 3 , pp. 171-180 . https://doi.org/10.2478/prolas-2020-0028
Abstract: Bone repair after surgical intervention on cleft lip palate (CLP) depends on the coordinated action of multiple tissue regeneration factors. We determined the relative number and appearance of tissue factors: matrix metalloproteinase-2 (MMP-2), tissue inhibitor of metalloproteinase-2 (TIMP-2), bone morphogenetic protein 2/4 (BMP 2/4), transforming growth factor beta (TGF-ß), Wnt3a protein (Wnt3a), Runt-related transcription factor 2 (Runx2), basic fibroblast growth factor (bFGF) and osteoprotegerin in hard tissue of CLP patients during first time surgical intervention. Fourtythree CLP patients with 24 bone and 36 cartilage samples were involved. Immunohistochemistry was used to assess the levels of tissue factors and the semi-quantitative census method was used for quantification of immunological structures. The increased amount of MMP-2 and bFGF positive cells was detected in the CLP group in cartilage and bone (p < 0.05), compared to the controls. A statistically significant (p = 0.012) increased amount of BMP 2/4 positive cells was found in cartilage of CLP patients, in comparison to the control group. Increased appearance of MMP-2, bFGF in hard tissue of the CLP patients indicates the predominance of tissue degradation. Increased number of BMP2/4 positive chondrocytes suggests improved cartilage growth and better regeneration in CLP patients.
Description: Funding Information: This study was funded by Riga Stradins University, No. 5-1/106/2019 from 12.04.2019, “The elaboration of diagnostic prognostic factor algorithm of facial tissue complex regeneration for cleft patients in longitudinal aspect”. Publisher Copyright: © 2020 Sciendo. All rights reserved. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
DOI: 10.2478/prolas-2020-0028
ISSN: 1407-009X
Appears in Collections:Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure

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