Please use this identifier to cite or link to this item:
10.1155/2017/7407136
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DC Field | Value | Language |
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dc.contributor.author | Latanova, Anastasia | - |
dc.contributor.author | Petkov, Stefan | - |
dc.contributor.author | Kuzmenko, Yulia | - |
dc.contributor.author | Kilpelaïnen, Athina | - |
dc.contributor.author | Ivanov, Alexander | - |
dc.contributor.author | Smirnova, Olga | - |
dc.contributor.author | Krotova, Olga | - |
dc.contributor.author | Korolev, Sergey | - |
dc.contributor.author | Hinkula, Jorma | - |
dc.contributor.author | Karpov, Vadim | - |
dc.contributor.author | Isaguliants, Maria | - |
dc.contributor.author | Starodubova, Elizaveta | - |
dc.date.accessioned | 2021-06-15T09:45:01Z | - |
dc.date.available | 2021-06-15T09:45:01Z | - |
dc.date.issued | 2017-06-22 | - |
dc.identifier.citation | Latanova , A , Petkov , S , Kuzmenko , Y , Kilpelaïnen , A , Ivanov , A , Smirnova , O , Krotova , O , Korolev , S , Hinkula , J , Karpov , V , Isaguliants , M & Starodubova , E 2017 , ' Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Effects on Its Immunogenic Performance in DNA-Immunized Mice ' , Journal of Immunology Research , vol. 2017 , 7407136 . https://doi.org/10.1155/2017/7407136 | - |
dc.identifier.issn | 2314-8861 | - |
dc.identifier.uri | https://dspace.rsu.lv/jspui/handle/123456789/5032 | - |
dc.description | Publisher Copyright: © 2017 Anastasia Latanova et al. | - |
dc.description.abstract | Reverse transcriptase (RT) is a key enzyme in viral replication and susceptibility to ART and a crucial target of immunotherapy against drug-resistant HIV-1. RT induces oxidative stress which undermines the attempts to make it immunogenic. We hypothesized that artificial secretion may reduce the stress and make RT more immunogenic. Inactivated multidrug-resistant RT (RT1.14opt-in) was N-terminally fused to the signal providing secretion of NS1 protein of TBEV (Ld) generating optimized inactivated Ld-carrying enzyme RT1.14oil. Promotion of secretion prohibited proteasomal degradation increasing the half-life and content of RT1.14oil in cells and cell culture medium, drastically reduced the residual polymerase activity, and downmodulated oxidative stress. BALB/c mice were DNA-immunized with RT1.14opt-in or parental RT1.14oil by intradermal injections with electroporation. Fluorospot and ELISA tests revealed that RT1.14opt-in and RT1.14oil induced IFN-γ/IL-2, RT1.14opt-in induced granzyme B, and RT1.14oil induced perforin production. Perforin secretion correlated with coproduction of IFN-γ and IL-2 (R=0,97). Both DNA immunogens induced strong anti-RT antibody response. Ldpeptide was not immunogenic. Thus, Ld-driven secretion inferred little change to RT performance in DNA immunization. Positive outcome was the abrogation of polymerase activity increasing safety of RT-based DNA vaccines. Identification of the molecular determinants of low cellular immunogenicity of RT requires further studies. | en |
dc.format.extent | 8887159 | - |
dc.language.iso | eng | - |
dc.relation.ispartof | Journal of Immunology Research | - |
dc.rights | info:eu-repo/semantics/openAccess | - |
dc.subject | 1.6 Biological sciences | - |
dc.subject | 1.1. Scientific article indexed in Web of Science and/or Scopus database | - |
dc.subject | Immunology and Allergy | - |
dc.subject | Immunology | - |
dc.subject | SDG 3 - Good Health and Well-being | - |
dc.title | Fusion to Flaviviral Leader Peptide Targets HIV-1 Reverse Transcriptase for Secretion and Reduces Its Enzymatic Activity and Ability to Induce Oxidative Stress but Has No Major Effects on Its Immunogenic Performance in DNA-Immunized Mice | en |
dc.type | /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article | - |
dc.identifier.doi | 10.1155/2017/7407136 | - |
dc.contributor.institution | Rīga Stradiņš University | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85022085933&partnerID=8YFLogxK | - |
dc.description.status | Peer reviewed | - |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
Files in This Item:
File | Size | Format | |
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Fusion_to_Flaviviral_Leader_Peptide_Targets_HIV_1_Reverse_Transcriptase_for_Secretion.pdf | 8.68 MB | Adobe PDF | View/Open |
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