Please use this identifier to cite or link to this item:
10.3390/cosmetics8020042
Title: | Characterization of macrophages and tnf-α in cleft affected lip tissue |
Authors: | Goida, Jana Pilmane, Māra Institute of Anatomy and Anthropology |
Keywords: | type 1 macrophages;type 2 macrophages;TNF-α;cleft lip and palate;children;3.1 Basic medicine;3.2 Clinical medicine;1.1. Scientific article indexed in Web of Science and/or Scopus database |
Issue Date: | 2021 |
Citation: | Goida , J & Pilmane , M 2021 , ' Characterization of macrophages and tnf-α in cleft affected lip tissue ' , Cosmetics , vol. 8 , no. 2 , 42 . https://doi.org/10.3390/cosmetics8020042 |
Abstract: | Orofacial clefts are one of the most common congenital anomalies worldwide; however, morphopathogenesis of the clefts is not yet completely understood. Taking the importance of innate immunity into account, the aim of this work was to examine the appearance and distribution of macrophages (M) 1, M2, and TNF-α, as well as to deduce any possible intercorrelations between the three factors in cleft affected lip tissue samples. Twenty samples of soft tissue were collected from children during plastic surgery. Fourteen control tissue samples were obtained during labial frenectomy. Tissues were immunohistochemically stained, analysed by light microscopy using a semi-quantitative method, and the Mann–Whitney U and Spearman’s tests were used to evaluate statistical differences and correlations. A statistically significant difference in the distribution was observed only in regard to M1. A weak correlation was observed between M2 and TNF-α but a moderate one between M1 and M2 as well as M1 and TNF-α. However, only the correlation between M1 and M2 was statistically important. The rise in M1, alongside the positive correlation between M1 and TNF-α, suggested a more pro-inflammatory/inflammatory environment in the cleft affected lip tissue. The moderate positive correlation between M1 and M2 indicated an intensification of the protective mechanisms. |
Description: | Funding Information: Funding: This research was funded by Riga Stradins University, Project 5-1/384/2020 “The complex research on the interrelation between tissue remodelling, tissue phenotype, and inflammation in most common craniofacial anomalies in Latvia”. Tissue samples were provided by Professor Ilze Akota. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. |
DOI: | 10.3390/cosmetics8020042 |
ISSN: | 2079-9284 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
Files in This Item:
File | Size | Format | |
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Characterization_of_macrophages_and_TNF_.pdf | 51.69 MB | Adobe PDF | View/Open |
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