1. DSpace at Riga Stradiņš University
  2. Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure
  3. Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure
Please use this identifier to cite or link to this item: 10.1016/j.stemcr.2019.03.003
Full metadata record
DC FieldValueLanguage
dc.contributor.authorEasterbrook, Jennifer-
dc.contributor.authorRybtsov, Stanislav-
dc.contributor.authorGordon-Keylock, Sabrina-
dc.contributor.authorIvanovs, Andrejs-
dc.contributor.authorTaoudi, Samir-
dc.contributor.authorAnderson, Richard A.-
dc.contributor.authorMedvinsky, Alexander-
dc.date.accessioned2021-04-16T06:35:01Z-
dc.date.available2021-04-16T06:35:01Z-
dc.date.issued2019-05-14-
dc.identifier.citationEasterbrook , J , Rybtsov , S , Gordon-Keylock , S , Ivanovs , A , Taoudi , S , Anderson , R A & Medvinsky , A 2019 , ' Analysis of the Spatiotemporal Development of Hematopoietic Stem and Progenitor Cells in the Early Human Embryo ' , Stem Cell Reports , vol. 12 , no. 5 , pp. 1056-1068 . https://doi.org/10.1016/j.stemcr.2019.03.003-
dc.identifier.issn2213-6711-
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3817-
dc.descriptionFunding Information: We thank the patients for donating tissue and A. Saunderson, J. Creiger, and N. Forson for gaining consent from patients and collecting specimens. We thank J. Verth, C. Manson, and their staff for animal maintenance. This work was supported by the Wellcome Trust (grant 100468/Z/12/Z , J.E.) and the MRC (grant G0901577 , A.M.). Publisher Copyright: © 2019-
dc.description.abstractDefinitive hematopoietic stem cells (HSCs)first emerge in the aorta-gonad-mesonephros (AGM)region in both mice and humans. An ex vivo culture approach has enabled recapitulation and analysis of murine HSC development. Knowledge of early human HSC development is hampered by scarcity of tissue: analysis of both CFU-C and HSC development in the human embryo is limited. Here, we characterized the spatial distribution and temporal kinetics of CFU-C development within early human embryonic tissues. We then sought to adapt the murine ex vivo culture system to recapitulate human HSC development. We show robust expansion of CFU-Cs and maintenance, but no significant expansion, of human HSCs in culture. Furthermore, we demonstrate that HSCs emerge predominantly in the middle section of the dorsal aorta in our culture system. We conclude that there are important differences between early mouse and human hematopoiesis, which currently hinder the quest to recapitulate human HSC development ex vivo.en
dc.format.extent13-
dc.format.extent1944067-
dc.language.isoeng-
dc.relation.ispartofStem Cell Reports-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.subjectfetal liver-
dc.subjecthematopoietic progenitors-
dc.subjecthematopoietic stem cells-
dc.subjecthuman embryo-
dc.subjectyolk sac-
dc.subject3.1 Basic medicine-
dc.subject3.2 Clinical medicine-
dc.subject1.6 Biological sciences-
dc.subject3.3 Health sciences-
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database-
dc.subjectBiochemistry-
dc.subjectGenetics-
dc.subjectDevelopmental Biology-
dc.subjectCell Biology-
dc.subjectSDG 3 - Good Health and Well-being-
dc.titleAnalysis of the Spatiotemporal Development of Hematopoietic Stem and Progenitor Cells in the Early Human Embryoen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article-
dc.identifier.doi10.1016/j.stemcr.2019.03.003-
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85065158624&partnerID=8YFLogxK-
dc.description.statusPeer reviewed-
Appears in Collections:Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure

Files in This Item:
File SizeFormat 
Analysis_of_the_Spatiotemporal_Development.pdf1.9 MBAdobe PDFView/Openopen_acces_unlocked
Show simple item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.