Please use this identifier to cite or link to this item: 10.2478/prolas-2019-0046
Title: Serodiagnosis of human bocavirus 1 infection among hospitalised children with lower respiratory tract infection in Latvia
Authors: Ziemele, Inga
Xu, Man
Vilmane, Anda
Rasa-Dzelzkaleja, Santa
Hedman, Klaus
Söderlund-Venermo, Maria
Gardovska, Dace
Nora-Krukle, Zaiga
Murovska, Modra
Department of Paediatrics
Institute of Microbiology and Virology
Keywords: children;cytokines;human bocavirus;lower respiratory tract infection;serology;1.6 Biological sciences;3.2 Clinical medicine;1.1. Scientific article indexed in Web of Science and/or Scopus database;General;SDG 3 - Good Health and Well-being
Issue Date: 1-Aug-2019
Citation: Ziemele , I , Xu , M , Vilmane , A , Rasa-Dzelzkaleja , S , Hedman , K , Söderlund-Venermo , M , Gardovska , D , Nora-Krukle , Z & Murovska , M 2019 , ' Serodiagnosis of human bocavirus 1 infection among hospitalised children with lower respiratory tract infection in Latvia ' , Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences , vol. 73 , no. 4 , pp. 288-295 .
Abstract: Since its discovery in 2005, human bocavirus 1 (HBoV1) has globally been one of the most common respiratory viruses. It is currently accepted that HBoV1 is a pathogen, causing upper and lower respiratory tract infections (LRTIs) in children. However, due to the prolonged HBoV1 DNA shedding from the upper airways and the subsequent high rate of co-detections with other respiratory viruses, the interpretation of positive polymerase chain reaction results is challenging. The aim of this study was to identify acute HBoV1 infections by the presence of HBoV1-specific IgM and IgG measured by competition enzyme immunoassay, to elucidate the induction of Th1/Th2 cytokines, and to describe the clinical characteristics associated with acute HBoV1 infection in hospitalised children less than five years of age with LRTI. HBoV1 IgM was detected in 19/102 (18.6%) and IgG in 66/102 (64.7%) patients. HBoV1 IgM was most frequently found in patients aged 13 to 24 months. Pneumonia and acute wheezing were the most common clinical diagnoses among HBoV1 IgM positive patients. The seroprevalence of HBoV1-specific IgG increased with age, reaching 85% by the age of five years. INF-γ, IL-4, IL-5, and IL-10 were observed to be higher in patients with acute HBoV1 infection.
Description: Funding Information: We thank Lea Hedman for technical advice in serology. This study was supported in part by the Rîga Stradiòð University research project “Epidemiology, pathogenicity of human bocavirus (HBoV) types and their possible connection to lower respiratory tract diseases and acute gastroenteritis in children”, RSU ZP 17/2013, National Research Programme “Biomedicine” project Nr. 5.6.2, the Sigrid Jusélius Foundation, the Life and Health Medical Grant Association, the Jane and Aatos Erkko Foundation, the Medical Society of Finland (FLS), and The Finnish Society of Sciences and Letters. Publisher Copyright: © 2019 Inga Ziemele et al., published by Sciendo 2019. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
DOI: 10.2478/prolas-2019-0046
ISSN: 1407-009X
Appears in Collections:Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure

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