Please use this identifier to cite or link to this item: 10.3389/fcimb.2024.1335096
Title: Genomic and phenotypic inconsistencies in Pseudomonas aeruginosa resistome among intensive care patients
Authors: Dolguševs, Mihails
Jain, Nityanand
Savicka, Oksana
Vangravs, Reinis
Bodrenko, Jevgenijs
Bergmanis, Edvins
Zemite, Dace
Selderina, Solvita
Reinis, Aigars
Rozentāle, Baiba
Department of Doctoral Studies
Statistics Unit
Department of Infectology
Department of Biology and Microbiology
Department of Public Health and Epidemiology
Keywords: Pseudomonas aeruginosa;environmental reservoirs;Intensive care units;Antimicrobial resistance;antimicrobial susceptibility testing;Next-Generation Sequencing;3.2 Clinical medicine;2.8 Environmental biotechnology;3.1 Basic medicine;1.6 Biological sciences;3.3 Health sciences;1.1. Scientific article indexed in Web of Science and/or Scopus database;Medicine(all);Immunology and Microbiology(all);Environmental Science(all);Pharmacology, Toxicology and Pharmaceutics(all);SDG 3 - Good Health and Well-being
Issue Date: 21-Jun-2024
Citation: Dolguševs , M , Jain , N , Savicka , O , Vangravs , R , Bodrenko , J , Bergmanis , E , Zemite , D , Selderina , S , Reinis , A & Rozentāle , B 2024 , ' Genomic and phenotypic inconsistencies in Pseudomonas aeruginosa resistome among intensive care patients ' , Frontiers in Cellular and Infection Microbiology , vol. 14 , 1335096 , pp. 1-12 . https://doi.org/10.3389/fcimb.2024.1335096
Abstract: Objective: Pseudomonas aeruginosa, a difficult-to-manage nosocomial pathogen, poses a serious threat to clinical outcomes in intensive care (ICU) patients due to its high antimicrobial resistance (AMR). To promote effective management, it is essential to investigate the genomic and phenotypic differences in AMR expression of the isolates. Methods: A prospective observational study was conducted from July 2022 to April 2023 at Liepaja Regional Hospital in Latvia. The study included all adult patients who were admitted to the ICU and had a documented infection with P. aeruginosa, as confirmed by standard laboratory microbiological testing and short-read sequencing. Since ResFinder is the only sequencing-based database offering antibacterial susceptibility testing (AST) data for each antibiotic, we conducted a comparison of the resistance profile with the results of phenotypic testing, evaluating if ResFinder met the US Food and Drug Administration (FDA) requirements for approval as a new AMR diagnostic test. Next, to improve precision, AST data from ResFinder was compared with two other databases – AMRFinderPlus and RGI. Additionally, data was gathered from environmental samples to inform the implementation of appropriate infection control measures in real time. Results: Our cohort consisted of 33 samples from 29 ICU patients and 34 environmental samples. The presence of P. aeruginosa infection was found to be associated with unfavourable clinical outcomes. A third of the patient samples were identified as multi-drug resistant isolates. Apart from resistance against colistin, significant discrepancies were observed when phenotypic data were compared to genotypic data. For example, the aminoglycoside resistance prediction of ResFinder yielded a major errors value of 3.03% for amikacin, which was marginally above the FDA threshold. Among the three positive environmental samples, one sample exhibited multiple AMR genes similar to the patient samples in its cluster. Conclusion: Our findings underscore the importance of utilizing a combination of diagnostic methods for the identification of resistance mechanisms, clusters, and environmental reservoirs in ICUs.
Description: Publisher Copyright: Copyright © 2024 Dolgusevs, Jain, Savicka, Vangravs, Bodrenko, Bergmanis, Zemite, Selderina, Reinis and Rozentale.
DOI: 10.3389/fcimb.2024.1335096
ISSN: 2235-2988
Appears in Collections:Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure

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