Please use this identifier to cite or link to this item:
10.5114/ceji.2023.133684
Title: | Association of human leukocyte antigen class II alleles with epithelial cell apoptosis and extracellular matrix production in acute COVID-19 |
Authors: | Vanaga, Ieva Kolesova, Oksana Kolesovs, Aleksandrs Sture, Gunta Hagina, Elvira Storozenko, Jelena Nikitina-Zake, Liene Vīksna, Ludmila Rīga Stradiņš University |
Keywords: | liver;hyaluronic acid;cytokeratin 18;HLA class II;COVID-19;apoptosis;3.2 Clinical medicine;1.1. Scientific article indexed in Web of Science and/or Scopus database;Immunology and Allergy;Immunology |
Issue Date: | 1-Jan-2023 |
Citation: | Vanaga , I , Kolesova , O , Kolesovs , A , Sture , G , Hagina , E , Storozenko , J , Nikitina-Zake , L & Vīksna , L 2023 , ' Association of human leukocyte antigen class II alleles with epithelial cell apoptosis and extracellular matrix production in acute COVID-19 ' , Central European Journal of Immunology , vol. 48 , no. 4 , pp. 1-8 . https://doi.org/10.5114/ceji.2023.133684 |
Abstract: | Introduction: Pathogenic mechanisms and long-term consequences of COVID-19 require attention in studies on SARS-CoV-2. The association of the severity of COVID-19 with genetic factors, such as human leukocyte antigen (HLA) genes, remains underexplored. Our study assessed the relationships between HLA class II alleles and COVID-19 severity and blood-based indicators of systemic inflammation and organ damage, serum markers of epithelial cell apoptosis such as caspase-cleaved CK18 fragment M30 (CK18-M30) and the extracellular matrix product hyaluronic acid (HA). Material and methods: The study included 101 hospitalized COVID-19 patients (mean age 60 ±14 years). Clinical tests were performed at admission to the hospital. The levels of CK18-M30 and HA were detected in serum by enzyme-linked immunosorbent assay (ELISA). HLA typing was performed in HLA-DRB1, -DQA1, and -DQB1 loci by the polymerase chain reaction with low-resolution sequence-specific primers. Results: Sixty-one patients had a non-severe and 40 had a severe or critical disease course (following the WHO definition). The severity was associated with older age, male gender, higher HA, CK18-M30, and some indicators of inflammation. Despite the lack of direct association between HLA alleles and the severity of COVID-19, the presence of HLA-DRB1*04 and 12 alleles in the genotype was associated with lowered or elevated HA, respectively. The HLA-DQB1*03:01 allele was associated with lowered CK18-M30, aspartate aminotransferase, and ferritin. In addition, HLA-DQB1*06:01 was associated with elevated alanine aminotransferase. Conclusions: Associations of HLA class II alleles with markers of epithelial cell apoptosis and extracellular matrix production indirectly support the influence of HLA genes on acute COVID-19 severity. |
Description: | Publisher Copyright: © 2023 Termedia Publishing House Ltd.. All rights reserved. |
DOI: | 10.5114/ceji.2023.133684 |
ISSN: | 1426-3912 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
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