Title: | Molecular profile of parathyroid tissues and tumours : a heterogeneous landscape |
Authors: | Uljanovs, Romans Strumfa, Ilze Bahs, Guntis Vidusa, Liga Merkurjeva, Kristine Franckevica, Ivanda Strumfs, Boriss Rīga Stradiņš University |
Keywords: | Heterogeneity;Parafibromin;Parathyroid adenoma;Primary parathyroid hyperplasia;Proliferation;3.1 Basic medicine;1.1. Scientific article indexed in Web of Science and/or Scopus database;Pathology and Forensic Medicine |
Issue Date: | Oct-2021 |
Citation: | Uljanovs , R , Strumfa , I , Bahs , G , Vidusa , L , Merkurjeva , K , Franckevica , I & Strumfs , B 2021 , ' Molecular profile of parathyroid tissues and tumours : a heterogeneous landscape ' , Polish Journal of Pathology , vol. 72 , no. 2 , pp. 99-116 . |
Abstract: | Advances in laboratory diagnostics and surgical treatment of primary hyperpara-thyroidism have ensured solid basis for research in parathyroid pathology in order to specify key molecules in pathogenesis and morphological diagnostics of difficult cases. The aim of this study was to assess the molecular landscape and its heterogeneity in primary parathyroid hyperplasia (PPH) and adenoma, compared to carcinoma and normal glands. In a retrospective analysis of 179 surgically removed parathyroid glands (102 adenomas; 27 PPH; 45 normal glands; 5 carci-nomas), expression of Ki-67, p21, p27, p53, cyclin D1, Bcl-2 protein, vimentin, cytokeratin (CK) 19, E-cadherin, CD56, CD44 and parafibromin was detected by immunohistochemistry, followed by computer-assisted assessment of mean values and heterogeneity measures. Descriptive statistics and Kruskal-Wallis test were applied. Significant differences were disclosed regarding the mean and highest fraction of Ki-67 (both p < 0.001), p21 (both p < 0.001), cyclin D1 (p = 0.002) and p27-expressing cells (p = 0.010). Proliferative lesions (PPH, adenoma and carcinoma) showed statistically significantly up-regulated CK19 (p = 0.012), decreased E-cadherin levels and distinctive patterns of vimentin. CD44, CD56 and p53 were almost absent from parathyroid tissues. All carcinomas lacked parafibromin contrasting with invariable positivity in adenomas. Remarkable heterogeneity of cell cycle markers and intermediate filaments must be accounted for in scientific studies and elaboration of diagnostic cut-offs. |
Description: | Funding Information: We sincerely thank MD, PhD Andrejs Vanags for financial support within the frames of research project Nr. 2013/0004/1DP/1.1.1.2.0/13/APIA/VIAA/020 ?Development of differential diagnostic technologies for neuroendocrine and endocrine tumours?, co-fi-nanced by the European Social Fund; and MD Ilze Fridrihsone for friendly and inspiring discussions. Publisher Copyright: © 2021, Termedia Publishing House Ltd.. All rights reserved. |
ISSN: | 1233-9687 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
Files in This Item:
File | Size | Format | |
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Molecular_profile_of_parathyroid_tissues_and_tumours.pdf | 5.23 MB | Adobe PDF | View/Open |
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