Molecular profile of parathyroid tissues and tumours : a heterogeneous landscape

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dc.contributor.authorUljanovs, Romans
dc.contributor.authorStrumfa, Ilze
dc.contributor.authorBahs, Guntis
dc.contributor.authorVidusa, Liga
dc.contributor.authorMerkurjeva, Kristine
dc.contributor.authorFranckevica, Ivanda
dc.contributor.authorStrumfs, Boriss
dc.contributor.institutionRīga Stradiņš University
dc.date.accessioned2023-11-22T11:25:01Z
dc.date.available2023-11-22T11:25:01Z
dc.date.issued2021-10
dc.descriptionFunding Information: We sincerely thank MD, PhD Andrejs Vanags for financial support within the frames of research project Nr. 2013/0004/1DP/1.1.1.2.0/13/APIA/VIAA/020 ?Development of differential diagnostic technologies for neuroendocrine and endocrine tumours?, co-fi-nanced by the European Social Fund; and MD Ilze Fridrihsone for friendly and inspiring discussions. Publisher Copyright: © 2021, Termedia Publishing House Ltd.. All rights reserved.
dc.description.abstractAdvances in laboratory diagnostics and surgical treatment of primary hyperpara-thyroidism have ensured solid basis for research in parathyroid pathology in order to specify key molecules in pathogenesis and morphological diagnostics of difficult cases. The aim of this study was to assess the molecular landscape and its heterogeneity in primary parathyroid hyperplasia (PPH) and adenoma, compared to carcinoma and normal glands. In a retrospective analysis of 179 surgically removed parathyroid glands (102 adenomas; 27 PPH; 45 normal glands; 5 carci-nomas), expression of Ki-67, p21, p27, p53, cyclin D1, Bcl-2 protein, vimentin, cytokeratin (CK) 19, E-cadherin, CD56, CD44 and parafibromin was detected by immunohistochemistry, followed by computer-assisted assessment of mean values and heterogeneity measures. Descriptive statistics and Kruskal-Wallis test were applied. Significant differences were disclosed regarding the mean and highest fraction of Ki-67 (both p < 0.001), p21 (both p < 0.001), cyclin D1 (p = 0.002) and p27-expressing cells (p = 0.010). Proliferative lesions (PPH, adenoma and carcinoma) showed statistically significantly up-regulated CK19 (p = 0.012), decreased E-cadherin levels and distinctive patterns of vimentin. CD44, CD56 and p53 were almost absent from parathyroid tissues. All carcinomas lacked parafibromin contrasting with invariable positivity in adenomas. Remarkable heterogeneity of cell cycle markers and intermediate filaments must be accounted for in scientific studies and elaboration of diagnostic cut-offs.en
dc.description.statusPeer reviewed
dc.format.extent18
dc.format.extent5358476
dc.identifier.citationUljanovs, R, Strumfa, I, Bahs, G, Vidusa, L, Merkurjeva, K, Franckevica, I & Strumfs, B 2021, 'Molecular profile of parathyroid tissues and tumours : a heterogeneous landscape', Polish Journal of Pathology, vol. 72, no. 2, pp. 99-116. https://doi.org/10.5114/pjp.2021.109513
dc.identifier.doi10.5114/pjp.2021.109513
dc.identifier.issn1233-9687
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/14968
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85116926665&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofPolish Journal of Pathology
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectHeterogeneity
dc.subjectParafibromin
dc.subjectParathyroid adenoma
dc.subjectPrimary parathyroid hyperplasia
dc.subjectProliferation
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectPathology and Forensic Medicine
dc.titleMolecular profile of parathyroid tissues and tumours : a heterogeneous landscapeen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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