Please use this identifier to cite or link to this item:
10.3390/v15051146
Title: | Cytokine Response Following SARS-CoV-2 Antigen Stimulation in Patients with Predominantly Antibody Deficiencies |
Authors: | Lucāne, Zane Šlisere, Baiba Gersone, Gita Papirte, Sindija Gailīte, Linda Tretjakovs, Pēteris Kurjāne, Nataļja Department of Biology and Microbiology Department of Internal Diseases Department of Human Physiology and Biochemistry Scientific Laboratory of Molecular Genetics |
Keywords: | 3.2 Clinical medicine;3.1 Basic medicine;1.1. Scientific article indexed in Web of Science and/or Scopus database |
Issue Date: | 10-May-2023 |
Citation: | Lucāne , Z , Šlisere , B , Gersone , G , Papirte , S , Gailīte , L , Tretjakovs , P & Kurjāne , N 2023 , ' Cytokine Response Following SARS-CoV-2 Antigen Stimulation in Patients with Predominantly Antibody Deficiencies ' , Viruses , vol. 15 , no. 5 , 1146 , pp. 1-23 . https://doi.org/10.3390/v15051146 |
Abstract: | Predominantly antibody deficiencies (PADs) are inborn disorders characterized by immune dysregulation and increased susceptibility to infections. Response to vaccination, including severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), may be impaired in these patients, and studies on responsiveness correlates, including cytokine signatures to antigen stimulation, are sparse. In this study, we aimed to describe the spike-specific cytokine response following whole-blood stimulation with SARS-CoV-2 spike peptides in patients with PAD (n = 16 with common variable immunodeficiency and n = 15 with selective IgA deficiency) and its relationship with the occurrence of coronavirus disease 2019 (COVID-19) during up to 10-month follow-up period. Spike-induced antibody and cytokine production was measured using ELISA (anti-spike IgG, IFN-γ) and xMAP technology (interleukin-1β (IL-1β), IL-4, IL-6, IL-10, IL-15, IL-17A, IL-21, TNF-α, TGF-β1). No difference was found in the production of cytokines between patients with PAD and controls. Anti-spike IgG and cytokine levels did not predict contraction of COVID-19. The only cytokine that distinguished between vaccinated and naturally infected unvaccinated PAD patients was IFN-γ (median 0.64 (IQR = 1.08) in vaccinated vs. 0.10 (IQR = 0.28) in unvaccinated). This study describes the spike-specific cytokine response to SARS-CoV-2 antigens, which is not predictive of contracting COVID-19 during the follow-up. |
Description: | Funding Information: This research was funded by the Latvian Council of Science project Nr.lzp-2020/1-0269. Publisher Copyright: © 2023 by the authors. |
DOI: | 10.3390/v15051146 |
ISSN: | 1999-4915 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
Files in This Item:
File | Size | Format | |
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Cytokine_Response_Following_SARS_CoV_2_Antigen_Stimulation.pdf | 2.88 MB | Adobe PDF | View/Open |
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