Browsing by Author "Pilmane, Mara"

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    Apoptosis and MMP-2, TIMP-2 expression in cleft lip and palate
    (2013) Smane, Liene; Pilmane, Mara; Akota, Ilze; Institute of Anatomy and Anthropology; Department of Morphology; Rīga Stradiņš University
    Aim of our study was complex detection of appearance and distribution of specific signalling proteins and apoptosis in facial tissue of children with complete bilateral cleft lip and palate (CBCLP). MATERIALS AND METHODS. Nineteen CBCLP patients and 11 unaffected subjects were involved in this study. All the tissue samples were proceeded for detection of matrix metalloproteinase-2 (MMP-2), tissue inhibitor of matrix metalloproteinase-2 (TIMP-2), and apoptosis. The intensity of immunostaining was graded semi-quantitatively. Results of the terminal deoxynucleotidyl transferase mediated dUTP nick-end labeling (TUNEL) method were obtained by counting apoptosis positive cells in five unintentionally chosen fields of vision. Groups were compared using the Mann-Whitney test. RESULTS. TUNEL-positive oral epithelial cells were significantly increased in the control group when compared with the CBCLP group. Connective tissue cells have a statistically significant lower expression of TIMP-2 in the control group compared to the CBCLP group. CONCLUSIONS. TIMP-2 positive connective tissue cells increasingly found in oral mucosa lamina propria proves the decrease of local apoptosis in CLP patients. Prominent expression of MMP-2 in cleft affected soft tissue indicates a possible increase of tissue remodelling.
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    Automatization of CT Annotation : Combining AI Efficiency with Expert Precision
    (2024-01-15) Edelmers, Edgars; Kazoka, Dzintra; Bolocko, Katrina; Sudars, Kaspars; Pilmane, Mara; Institute of Anatomy and Anthropology
    The integration of artificial intelligence (AI), particularly through machine learning (ML) and deep learning (DL) algorithms, marks a transformative progression in medical imaging diagnostics. This technical note elucidates a novel methodology for semantic segmentation of the vertebral column in CT scans, exemplified by a dataset of 250 patients from Riga East Clinical University Hospital. Our approach centers on the accurate identification and labeling of individual vertebrae, ranging from C1 to the sacrum–coccyx complex. Patient selection was meticulously conducted, ensuring demographic balance in age and sex, and excluding scans with significant vertebral abnormalities to reduce confounding variables. This strategic selection bolstered the representativeness of our sample, thereby enhancing the external validity of our findings. Our workflow streamlined the segmentation process by eliminating the need for volume stitching, aligning seamlessly with the methodology we present. By leveraging AI, we have introduced a semi-automated annotation system that enables initial data labeling even by individuals without medical expertise. This phase is complemented by thorough manual validation against established anatomical standards, significantly reducing the time traditionally required for segmentation. This dual approach not only conserves resources but also expedites project timelines. While this method significantly advances radiological data annotation, it is not devoid of challenges, such as the necessity for manual validation by anatomically skilled personnel and reliance on specialized GPU hardware. Nonetheless, our methodology represents a substantial leap forward in medical data semantic segmentation, highlighting the potential of AI-driven approaches to revolutionize clinical and research practices in radiology.
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    Barx1, growth factors and apoptosis in facial tissue of children with clefts
    (2008) Krivicka-Uzkurele, Benita; Pilmane, Mara; Akota, Ilze; Institute of Anatomy and Anthropology; Rīga Stradiņš University
    OBJECTIVE: Clefts of lip and palate belong to the most common birth defects worldwide. Growth factors and genes play an important role in tissue growth, differentiation and induction and upregulation of growth factors, apoptosis and matrix metalloproteinases might be involved in pathogenesis of facial clefts. The aim of this study was investigation of palate tissue in children with unilateral cleft lip palate for detection of local tissue growth factors, barx1 and apoptosis. MATERIALS AND METHODS:We investigated soft and hard palate tissue from 36 children with complete unilateral cleft lip and palate from cleft area.14 children were in age before and primary dentition, but 22 children were in mixed dentition period. We examined the localization of barx1, FGFR1, NGFR, TGFbeta, BMP2/4, MMP2, PGP 9,5 by immunohistochemistry. TUNEL method was performed for detection of apoptotic cells. RESULTS: Abundance of FGFR1 positive cells was seen almost in all cases. FGFR richly stained cells of soft and hard palate tissue. Abundance of NGFR positive cells was detected in basal epithelium, hair follicles, nerve fibers in wall of blood vessels and subepithelium, and was more often seen in children before mixed dentition. TGFbeta has showed intensive expression in epithelium, cartilage and bone in both dentition ages. Chondrocytes, fibroblasts and macrophages expressed MMP2 predominant before mixed dentition. Regional expression of barx1 was observed in epithelium before the mixed dentition, while during mixed dentition gene appeared in hyaline cartilage. TUNEL discovered apoptosis in both dentition ages. CONCLUSIONS: FGFR1 and TGFbeta are main tissue stimulating growth factors in both dentition ages. Expression of barx1 appears in cleft lip palate affected structures mainly in mixed dentition ages. NGFR and neuropeptides-containing structures are mainly characteristic in cleft tissue before mixed dentition. Distribution of genes, GF and apoptosis seem to correlate rather with dentition age than to type of CLP.
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    Body mass index, waist circumference and waist-to-hip-ratio in the prediction of obesity in Turkish teenagers
    (2014) Kavak, Vatan; Pilmane, Mara; Kazoka, Dzintra; Department of Morphology; Institute of Anatomy and Anthropology
    The aim of this study was to identify the usefulness of body mass index (BMI), waist circumference (WC) and waist- -to-hip ratio (WHR) in screening for obesity in teenagers by using the receiver operating characteristic (ROC). To select the sample set in this cross-sectional study, a stratified random sampling approach was utilized.Weight, height, WC, hip circumference and body fat percentage (BFP) were measured in 1118 children of both genders (597 boys and 521 girls), aged from 10 to 15 years old. Percentiles of BMI and Centers for Disease Control and Prevention-United States (CDCUS)- growth chart for boys and girls aged from 10 to 15 years old were presented. ROC analyses were then used to evaluate the performances of three anthropometric indices; BMI, WC and WHR had strong positive correlations with BFP (r=0.49–0.77) in both girls and boys within indicated age group. The area under the curves (AUCs) were high in both girls and boys for BMI, 0.795 and 0.893, respectively, and WC, 0.767 and 0.853, respectively, and were a little lower, 0.747 and 0.783, respectively, for WHR. In conclusion, this study demonstrates that the prevalence of being overweight and obese among teenagers of both sexes in our data set does not differ from CDC-US-growth chart. In addition, BMI and WC are two important predictors for teenagers to become overweight and obese, while WHR is less useful for this purpose.
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    Cell proliferation and apoptosis in wistar rat kidney after renal mass ablation and low-dose irradiation
    (2010) Aunapuu, Marina; Arend, Andres; Ots, Mai; Pilmane, Mara; Institute of Anatomy and Anthropology
    Cell proliferation and apoptosis in the remnant rat kidney after treatment with lowdose irradiation was investigated. Material and methods. In the first group (n=9), adult male Wistar rats underwent 5/6 nephrectomy (NPX); in the second group (n=9), NPX was combined with low-dose irradiation. Rats without surgery and irradiation formed the control group (n=9). Results. Hypertension and proteinuria induced by NPX were decreased by 3-Gy irradiation. The 5/6 NPX rats showed a dramatic increase in proliferating and apoptotic cells in the glomeruli and in the distal tubules at week 2, which was significantly decreased by low-dose irradiation. Conclusion. The data demonstrate that low-dose irradiation is a factor slowing the process of chronic renal injury.
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    Characterization of cytokines and proliferation marker Ki67 in cleft affected lip tissue
    (2019-09) Pilmane, Mara; Sidhoma, Elga; Akota, Ilze; Kazoka, Dzintra; Institute of Anatomy and Anthropology; Rīga Stradiņš University
    Background and objectives: Cleft lip palate takes the second place among all anomalies. The complex appearance of cytokines and proliferation markers has still not been clarified despite their possible crucial role in cleft tissue. Therefore, the aim of work was the detection of appearance of pro- and anti-inflammatory cytokines and proliferation marker Ki67, and their inter-correlations in cleft affected lip (CAL). Materials and Methods: The lip material was obtained from 16 children aged before primary dentition during plastic surgery. Control was obtained from 7 non-CAL oral tissue. Tissues were stained for IL-1, IL-4, IL-6, IL-8, IL-10 and Ki67 immunohistochemically. Nonparametric statistic, Mann-Whitney and Spearman’s coefficient were used. Results: All cytokines positive cells were observed more into the epithelium. Statistically significant difference was seen between epithelial IL-1, IL-10, IL-8 and Ki67 positive cells and IL-10-, IL-4-containing connective tissue cells in comparison to the control. Strong positive correlation was detected in CAL epithelium between IL-10 and IL-8, IL-10 and IL-4, IL-10 and IL-1, IL-1 and IL-8, IL-1 and IL-4, IL-4 and IL-8, IL-8 and Ki67, IL-10 and Ki67, but moderate-in connective tissue between IL-1 and IL-10, IL-1 and IL-4. Conclusion: The CAL epithelium is the main source for the interleukins. Rich similar expression of IL-1 and IL-10 suggests the balance between pro-and anti-inflammatory tissue response on basis of dysregulated tissue homeostasis (increase of IL-8). The correlations between the different ILs -1, -4, -8, -10 in CAL epithelium seem to indicate the self-protection compensatory mechanism for intensification of local inflammatory-immune response without involvement of IL-6. The correlations between Ki67 and cytokines indicate the involvement of IL-8 and IL-10 in stimulation of cellular proliferation. IL-4 and IL-10 expression from CAL connective tissue simultaneously to IL-1, IL-4 and IL-10 inter-correlations there suggests the intensification of local immune response regulated probably by main pro-inflammatory cytokine-IL-1.
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    Characterization of Pro- and Anti-Inflammatory Tissue Factors in Rosacea : A Pilot study
    (2020-12) Lohova, Elizabeta; Pilmane, Mara; Rone-Kupfere, Mara; Kisis, Janis; Institute of Anatomy and Anthropology
    Rosacea is a chronic inflammatory skin disease mainly affecting the facial skin. Our aim was to determine the appearance of pro- and anti- inflammatory cytokines in rosacea-affected facial tissue. Materials and Methods: Rosacea tissue were obtained from eight patients (aged 35 to 50 years). The control group (CG) included four facial skin samples (49 to 70 years). Routine staining and immunohistochemistry for IL-1, IL-10, LL-37, HBD-2, and HBD-4 proceeded. Results: Inflammation was observed in all the rosacea samples. A statistically significant difference was seen between epithelial HBD-2 positive cells in comparison to the control. There was a strong positive correlation between HBD-4 in the epithelium and HBD-4 in the connective tissue, IL-10 in the epithelium and IL-1 in the connective tissue, and IL-1 in the epithelium and IL-10 in the connective tissue. Conclusion: Increased levels of IL-10 and decreased levels of IL-1 show the balance between anti- and pro-inflammatory tissue responses. A significant amount of HBD-2 in the epithelium proves its important role in the local immune response of rosacea-affected tissue. The last effect seems to be intensified by the elevated level of LL-37 in the epithelium.
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    Cytokines, Proliferation Markers, Antimicrobial Factors and Neuropeptide-Containing Innervation in Human Nasal Mucosa after Rhinoseptoplasty Procedure
    (2021-04-21) Podlesnaja, Marija; Pilmane, Mara; Sumeraga, Gunta; Institute of Anatomy and Anthropology
    The nasal cavity lined by nasal mucosa, is a significant part of respiratory system of human. However, there are no studies aimed to detect a molecular phenotype of healthy and normal functioning nasal mucosa, obtained after rhinoseptoplasty procedure, to understand its physiology and growth and inflammation processes. Thus, our aim is to identify human healthy nasal mucosa cytokines, neuropeptide-containing innervation and cell proliferation markers to form a control group for further tissue investigation of human nasal polyposis as the next step of our research. The study included surgery materials from 17 healthy humans. Biotin-streptavidin immunohistochemistry was performed for detection of tissue PGP9.5, Ki-67, β-Defensin 2, IL-1, IL-4, IL-6, IL-7, IL-8, IL-10, IL-12. Results were evaluated semi-quantitatively and by Friedman ANOVA and Spearman rang correlation tests. All factors were more widely expressed by superficial epithelium than by glandular one. Abundance of ILs-8, -10 and -12 positive cells was detected in comparison with moderate to numerous distributions of IL-1, IL-6 and β-Defensin 2. Moderate number of PGP 9.5-containing nerve fibers and only few to moderate Ki-67 positive cells were found in healthy nasal mucosa. We revealed statistically significant difference between Ki-67 and ILs-4, -6, -7, -8, -10, -12 both in healthy nasal mucosa superficial and glandular epithelium. From nasal epithelia, commonly the surface one displays more cytokines and β-Defensin 2 in comparison to the glandular one. Numerous to abundant expression of ILs-4, -6, -7, -8, -10, -12 and β-Defensin 2 in nasal superficial and glandular epithelia proves probably these factors' role into the common immune response of tissue and stimulation of immune cell differentiation.
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    Detection of TGF-β1, HGF, IGF-1 and IGF-1R in cleft affected mucosa of the lip
    (2017-09-01) Sidhom, Elga; Pilmane, Mara; Institute of Anatomy and Anthropology
    Background: Orofacial clefts are one of the most common birth defects with multifactorial and only partly understood morphopathogenesis. Objective: The aim of this study was to evaluate the presence of TGF-β1, HGF, IGF-1 and IGF-1R in cleft affected mucosa of the lip. Methods: Lip mucosa tissue samples were obtained during surgical cleft correction from seven 2 to 6 months old children. Prepared tissue sections were stained by immunohistochemistry for TGF-β1, HGF, IGF-1 and IGF-1R. The intensity of staining was graded semiquantitatively. Results: We found numerous TGF-β1 and HGF-containing epithelial and connective tissue cells, moderate number of IGF-1 immunoreactive cells and even less pronounced presence of IGF-1R-positive cells. Conclusion: TGF-β1 and HGF are present in defective epithelia and soft tissue in cleft affected lip. Expressions of IGF-1 and IGF-1R show significant differences, and both factors play a role in the morphopathogenesis of clefts.
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    Different Techniques of Creating Bone Digital 3D Models from Natural Specimens
    (2022-08) Edelmers, Edgars; Kažoka, Dzintra; Bolocko, Katrina; Pilmane, Mara; Institute of Anatomy and Anthropology
    The choice of technique for the creation of a 3D digital human bone model from natural specimens has a critical impact on the final result and usability of the obtained model. The cornerstone factor in 3D modeling is the number of faces of polygon mesh, along with topological accuracy, as well as resolution and level of detail of the texture map. Three different techniques (3D scanning, photogrammetry, and micro-computed tomography) have been used to create a digital 3D model of the human zygomatic bone. As implementation and use of 3D models can be divided into three main categories—visualization, simulation, and physical replication to obtain a functioning model (implant or prothesis)—the obtained models have been evaluated by the density and topological accuracy of the polygonal mesh, as well as by visual appearance by inspecting the obtained texture map. The obtained data indicate that for biomedical applications and computer biomechanical simulation the most appropriate technique of 3D model obtainment is micro-computed tomography, in its turn for visualization and educational purposes, the photogrammetry technique is a more preferable choice.
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    Early morphofunctional response of contact tissue after intraosal implantation in rabbit jaw of pure synthetic hydroxyapatite (HAp) bioceramic materials and HAp saturated with lidocaine
    (2009) Salma, Ilze; Pilmane, Mara; Skagers, Andrejs; Vetra, Janis; Salms, Girts; Berzina-Cimdina, Liga; Serzane, Rita; Department of Oral and Maxillofacial Surgery and Oral Medicine; Institute of Anatomy and Anthropology
    PURPOSE: The aim of this study was to evaluate early morphofunctional response of contact tissue to pure and saturated with lidocaine porous synthetic HAp ceramic implants inserted in holes of rabbit upper jaw. MATERIALS AND METHODS: Pure and saturated with lidocaine cylinders of porous HAp ceramics produced at Biomaterials R&D Laboratory of Riga Technical University were inserted into edentulous part of the upper jaw of 12 New Zealand male rabbits. After two weeks, blocks of soft tissue and bone were taken out. The sections done with EXACT Grunding system and from paraffin blocks were stained with haematoxylin/eosin and by use of biotin-streptavidin immunohistochemistry for transforming growth factor beta1 (TGFbeta1), fibroblast growth factor receptor 1 (FGFR1), interleukin 10 (IL10). Apoptosis was detected by TUNEL method. RESULTS: TGFbeta1 was equally expressed in the bone around HAp implants saturated with lidocaine and without lidocaine, while control sections did not show any expression of TGFbeta. In contacting bone tissue to pure HAp and HAp saturated with lidocaine as well in controls rich expression of FGFR1 was observed. In soft tissue of experimental samples also rich expression of FGFR1 was observed, although it was weak in control samples. IL10 had rich expression in all samples. The mean number of apoptotic cells was higher in samples with pure HAp, slightly decreased in samples HAp with lidocaine and significantly lower in controls. CONCLUSIONS: The expression of TGF beta1 confirms osteoinductive activity of HAp. Contact soft tissue to HAp showed rich FGFR1 expression, and weak in controls suggesting higher plasticity of connective tissue to implants in comparison with supportive tissue. Slightly lower apoptosis in samples of HAp with lidocaine gives evidence of indistinct influence of saturated with lidocaine HAp implants on development of programmed cell death.
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    Evaluation of Histological Findings of Airways of Rats Exposed to Highly Polluted Indoor Air in Offices
    (2016-10-01) Martinsone, Žanna; Pilmane, Mara; Moisejevs, Georgijs; Sprudža, Dagmara; Baķe, Marite Arija; Institute of Occupational Safety and Environmental Health; Institute of Anatomy and Anthropology; Bioķīmijas zinātniskā laboratorija
    Humans are exposed to chemicals and PM (particulate matter), including ultrafine particles (nanoparticles), mainly through inhalation. This creates a risk to their health. Another effect to exposure is expression of cytokines and their role in lung inflammation and morphpathogenesis. We conducted a pilot project based on testing of realistic exposure scenarios by describing morphological changes of the respiratory tract in Wistar rats (male) during a 30-day exposure in office where there was high intensity of printing activities. Tracheal tissue of experimental animals had increasing concentrations of inflammatory interleukin IL-1 and decreasing concentrations of IL-6 and TNF-α. The lungs of the experimental animals tended to show focal infiltration of inflammatory cells, vascular plethora, focal and/or diffuse localisation of lymphatic nodules, and also vascular sclerosis and focal emphysema. The results suggested potential dangerous and adverse effect of poor indoor air quality (particles, including nanoparticles, and chemical compounds) on respiratory tract tissue of rats.
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    Evaluation of the presence of MMP-2, TIMP-2, BMP2/4, and TGFβ3 in the facial tissue of children with cleft lip and palate
    (2018) Smane, Liene; Pilmane, Mara; Institute of Anatomy and Anthropology
    Cleft lip and palate (CLP) is the most common defect affecting the face. The treatment consists of surgical reconstruction of the anatomical structures of the cleft. Part of the surgical treatment is reconstruction of the alveolar bone by means of autogenic bone grafting (osteoplasty). This study aimed to evaluate the levels of expression of extracellular matrix remodeling factors in the facial tissue of children with a complete unilateral (CU) and a complete bilateral (CB) CLP to assess whether the wound healing process is adequate. Twenty-two CLP patients were enrolled in this study. Tissue samples were collected during alveolar osteoplasty for unilateral (n = 12) or bilateral (n = 10) cleft palate, (age range from 6 years 8 months to 12 years 2 months). Control material was obtained in the case of tooth extraction (age range from 6 years 9 months to 14 years 5 months). Immunohistochemistry was used to assess the levels of matrix metalloproteinase-2 (MMP-2), tissue inhibitor of metalloproteinase-2 (TIMP-2), bone morphogenetic proteins 2 and 4 (BMP2/4), and transforming growth factor β3 (TGFβ3). Numbers of positively stained cells were graded semi-quantitatively. Data were analysed using the Kraskel-Wallis rank test and the Bonferroni correction. The total number of MMP2-positive cells was significantly lower in the CBCLP and in the control group than in the CUCLP (p < 0.001 after the Bonferroni correction). The total number of TIMP2-positive cells was significantly higher in the CUCLP than in the CBCLP and in the control group (p < 0.001; p < 0.003 after the Bonferroni correction). The overall number of BMP2/4, TGFβ3-positive cells was significantly higher in the CUCLP than in the CBCLP and in the control group (p < 0.001 after the Bonferroni correction). The decrease of the relative amount of statistically significant BMP2/4, TGFβ3, MMP-2, TIMP-2 containing bone cells in CBCLP patients identifies affected alveolar bone regeneration and remodeling process.
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    Expresión de IRF6, RYK y PAX9 en el tejido facial de niños con paladar fisurado
    (2015-06-01) Smane, Liene; Pilmane, Mara; Institute of Anatomy and Anthropology; Department of Morphology
    Cleft lip and palate (CLP) is a congenital anomaly characterized by the inappropriate fusion of the upper lip, alveolus, and secondary palate. This study investigated whether expression of interferon regulatory fac tor 6 (IRF6), receptor-like tyrosine kinase (RYK), and paired-box 9 (PAX9), which are essential for the normal development and morphogenesis of craniofacial structures, is dysregulated in children with CLP. Oral mucosa tissue samples were obtained from patients with complete bilateral (CB) CLP (n= 19) during corrective plastic surgery and unaffected control subjects (n= 7). IRF6, RYK, and PAX9 expression was assessed by immunohistochemistry, and data were analyzed with the Mann-Whitney test. In patients, IRF6 immunoreactivity in the connective tissue was moderate to high, but the overall number of IRF6-positive oral epithelial cells was lower than that in controls (z= -3.41; P= 0.01). RYK expression was observed only sporadically in the oral epithelium of 4 patients, in contrast to the control group (z= -3.75; P< 0.001). PAX9-positive epithelial cells were present in low to moderate numbers in patients with CBCLP, while an abundance of these cells was observed in the basal layer of the oral epithelium in controls (z= -3.60; P<0.001). IRF6 is the main connective tissue regulatory factor in CBCLP, and its low level of expression in the oral epithelium suggests a reduced potential for epitheliocyte differentiation, while low PAX9 and RYK expression may explain the decreased cell migration and cleft remodeling in CBCLP.
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    Expression of antimicrobial peptides in nail psoriasis and normal nails
    (2017) Saulite, Ieva; Pilmane, Mara; Kisis, Janis; Institute of Anatomy and Anthropology; Department of Dermatology and Venereology
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    Expression of gene proteins, interleukins and β-defensin in cleft-affected tissue
    (2017) Jankovska, Iveta; Pilmane, Mara; Akota, Ilze; Rīga Stradiņš University; Department of Morphology; Institute of Anatomy and Anthropology
    OBJECTIVES: The aim of the study was to investigate cleft - affected tissues in children with cleft lip and palate in order to detect appearance of β-defensin, interleukins (IL) and gene proteins. MATERIAL AND METHODS: The study group included 10 patients with cleft lip and palate. During nose surgery tissue samples from bone and cartilage have been taken, and stained with immunohistochemistry for β-defensin 2, IL4, IL6, IL7, IL8, IL10 and MSX1, RYK, PAX9, IRF6 gene proteins. RESULTS: Results showed prominent expression of IL10 (mean value 47.28±4.26 in visual field) followed by IL7 (35.62±11.18) in cartilage of patients, but slightly less pronounced expression of IL8 (30.14±8.74), IL6 (22.52±10.88) and IL4 (14.81±6.94). The expression of β-defensin 2 was prominent (34.52±11.79) and similar to expression of IL7 in tissue samples of cartilage. MSX1, PAX9, RYK and IRF6 (17.67±5.94; 16.14±5.52; 16.57±5.22 and 11.86±4.21) in cartilage was less pronounced than interleukins and β-defensin 2. MSX1 (12.44±3.34), PAX9 (6.89±2.14), RYK (11.0±5.92) and IRF6 (9.1±4.76) gene proteins expression in bone showed mostly rare occurrence of positive structures. CONCLUSIONS: Significant expression of IL7 and β-defensin 2 and IL10 in cartilage proves the prominent immune response in cleft affected hard tissue. Mostly indistinct MSX1, PAX9, RYK and IRF6 gene proteins expression in bone might be an indicator of not complete cellular differentiation, proliferation and migration events in cleft disordered hard tissue.
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    Expression of growth factors and growth factor receptors in human cleft-affected tissue
    (2013) Krivicka, Benita; Pilmane, Mara; Akota, Ilze; Institute of Anatomy and Anthropology; Department of Oral and Maxillofacial Surgery and Oral Medicine
    OBJECTIVE. To investigate cleft disordered tissue in children with cleft palate and cleft lip with or without alveolar clefting for detection of local tissue growth factors and growth factor receptors and compare findings. Design. Morphological analysis of human tissue. Patients. Three groups were studied: 14 patients with cleft palate at the age from eight months to 18 years and two months, 12 patients with cleft lip with or without alveolar clefting in the age from four months to 15 years and four months and 11 control patients. RESULTS. In general, cleft palate disordered tissue showed more prominent expression of BMP2/4 (z=3.574; p=0.0004) and TGFβ (z=2.127; p=0.033), while expression of TGFBR3 significantly higher was only in connective tissue (z=3.822; p=0.0001). Cleft lip affected tissue showed significantly pronounced expression of FGFR1 in general as well as separately in epithelium. CONCLUSIONS. The marked and statistically significant expression of BMP 2/4 in cleft palate disordered soft tissue probably is delayed, but still proliferation and differentiation as well as tissue, especially, bone remodeling contributing signal. Cleft palate affected tissue show more prominent expression of TGFβ, still the weak regional expression of TGFβ type III receptors prove the disordered tissue growth and changed TGFβ signalling pathway in postnatal pathogenesis. In general, expression of TGFβ, BMP 2/4 and FGFR1 is significantly different, giving evidence to the involvement of these mentioned factors in the cleft severity morphopathogenesis.
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    Expression of interferon regulatory factor 6, muscle segment homeobox 1, paired box gene 9, homeo box B3, and related to tyrosine kinases in human cleft-affected tissue
    (2016-01-01) Krivicka-Uzkurele, Benita; Pilmane, Mara; Department of Morphology; Institute of Anatomy and Anthropology
    Background and Aim: Recent studies demonstrate direct roles of different genes during formation of secondary palate, but there are no still data about local expression and distribution of gene products in cleft palate affected human tissue. Thus, the aim of our study was to investigate cleft disordered cartilage and bone for detection of local expression of key regulators of palatogenesis and its correlations. Materials and Methods: The study involved 16 patients with unilateral cleft lip and palate. Tissue samples were proceeded for detection of interferon regulatory factor 6 (IRF6), muscle segment homeobox 1 (MSX1), paired box gene 9 (PAX9), homeo box B3 (HOXB3), and related to tyrosine kinases with biotin-streptavidin immunohistochemistry. Distribution of immunoreactive structures was detected semiquantitatively. Statistical analysis included the Mann-Whitney test and Pearson′s correlation test. Results: Statistically significant differences were found between expression of IGFR6, MSX1, and HOXB3 in the cartilage and bone. We also detected statistically significant correlation between the expressions of PAX9 and MSX1 in the bone tissue. Conclusions: Cleft lip and palate disordered cartilage is characterized by more pronounced expression of IRF6, MSX1, and PAX9. Expression of HOXB3 is more characteristic for cleft lip and palate affected bone. Considered as a whole, our results suggest that the cleft lip and palate affected cartilage seems more plastic in tissue remodeling what can probably result in qualitative postoperative tissue reconstruction.
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    Expression of progesterone receptors in bovine corpus luteum during pregnancy
    (2004) Tamane, Regina; Pilmane, Mara; Jemeļjanovs, Aleksandrs; Dabuzinskiene, Anita; Institute of Anatomy and Anthropology
    The expression of progesterone receptors and matrix ribonucleic acid in 37 corpora lutea in 1-7 month's pregnant cows was investigated. Corpora lutea were obtained from slaughtered animals. Progesterone receptors expression was confirmed using biotin-streptavidin immunohistochemistry. Matrix ribonucleic acid expression was studied using pyronin staining by Unna-Brashe method. The expression of progesterone receptors was the highest in the luteal cells of the one-month pregnancy corpus luteum. It decreased significantly (p<0.05) from the third to the fourth month of pregnancy, but there was no significant difference in the expression of progesterone receptors from the first to the second and from the second to the third month of pregnancy. Also, there was no significant difference in the expression of progesterone receptors from the fourth to the fifth month and from the fifth to the sixth month of pregnancy. The expression of matrix ribonucleic acid also decreased during pregnancy, but gradually, with no significant monthly differences. It decreased significantly (p<0.05) from the sixth to the seventh month of pregnancy only. Our study shows that both indices - progesterone receptors and matrix ribonucleic acid decrease in the steroidogenic luteal cells during pregnancy and that a strong linear correlation (r=0.88) exists between these indices.
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    Growth factors, apoptotic cells and barx1 gene in bone and soft tissue of skeletal class III patients
    (2007) Jankovska, Iveta; Pilmane, Mara; Urtane, Ilga; Bigestans, Andris; Salms, Girts; Lauskis, Gunars; Department of Orthodontics; Institute of Anatomy and Anthropology; Department of Oral and Maxillofacial Surgery and Oral Medicine
    Growth factors and growth stimulating genes are main signaling molecules for growth and development in ante- and postnatal period involved in cellular proliferation, differentiation and morphogenesis of tissues and organs during embryogenesis, postnatal growth and adulthood. The aim of this study was to evaluate TGF-beta (transforming growth factor-beta), BMP2/4 (bone morphogenetic protein 2/4), FGFR1 (fibroblast growth factor receptor one), barx1 gene and apoptosis from tissue samples of oro-maxillo-facial region in skeletal class III patients to reveal possible morphopathogenesis of severe skeletal anomalies. The study group included 9 patients with skeletal class III malocclusion. During orthognatic surgery tissue samples from tuber maxillae, ramus mandibulae anterior and posterior part, as well as gingiva from the lower jaw in region of second molar have been taken. Samples were stained with immunohistochemistry for TGF-beta, BMP2/4, FGFR1, apoptosis and barx1 gene. We used also the routine histological staining with haematoxyline and eosine. In tuber maxillae, ramus mandibulae anterior and posterior part staining for TGF-beta was the most relevant. Also BMP2/4, FGFR1 and barx1 showed the highest mean number of positive cells in tuber maxillae. Barx1 was equally expressed in ramus mandibulae, but BMP2/4 and FGFR1 mainly stained its posterior part cells. Apoptosis mostly affected ramus mandibulae anterior part. CONCLUSIONS: We suggest about more active stimulation of bone growth in tuber maxilla whereas ramus mandibulae. Apoptosis mainly affects ramus mandibulae anterior part that possibly connects to the lower expression of growth stimulating factors and may indicate lower bone remodelation ability.