Please use this identifier to cite or link to this item:
10.1046/j.1365-313X.1999.00404.x
Title: | Re-evaluation of phytohormone-independent division of tobacco protoplast-derived cells |
Authors: | Schell, Jeff Bisseling, Ton Dülz, Marion Franssen, Henk Fritze, Klaus John, Michael Kleinow, Tatjana Leßnick, Angela Miklashevichs, Edvins Pawlowski, Katharina Röhrig, Horst Van De Sande, Karin Schmidt, Jürgen Steinbiß, Hans Henning Stoll, Marion Institute of Microbiology and Virology |
Keywords: | 1.6 Biological sciences;1.1. Scientific article indexed in Web of Science and/or Scopus database;Genetics;Plant Science;Cell Biology |
Issue Date: | Mar-1999 |
Citation: | Schell , J , Bisseling , T , Dülz , M , Franssen , H , Fritze , K , John , M , Kleinow , T , Leßnick , A , Miklashevichs , E , Pawlowski , K , Röhrig , H , Van De Sande , K , Schmidt , J , Steinbiß , H H & Stoll , M 1999 , ' Re-evaluation of phytohormone-independent division of tobacco protoplast-derived cells ' , Plant Journal , vol. 17 , no. 5 , pp. 461-466 . https://doi.org/10.1046/j.1365-313X.1999.00404.x |
Abstract: | We have used a [3H] thymidine incorporation assay and microscopic observation in order to reassess recently published data dealing with the response of tobacco protoplasts to phytohormones, lipochitooligosaccharides and peptides (Harling et al., 1997; Hayashi et al., 1992; Miklashevichs et al., 1996; Miklashevichs et al., 1997; Rohrig et al., 1995; Rohrig et al., 1996; van de Sande et al., 1996; Walden et al., 1994). These proliferation assays reveal that, in contrast to published data, isolated cells of the investigated mutant plant lines axi159 (Hayashi et al., 1992; Walden et al., 1994), axi4/1 (Harling et al., 1997) and cyil (Miklashevichs et al., 1997), which were generated by activation T-DNA tagging, were unable to grow in the absence of auxin or cytokinin. Furthermore, lipochitooligosaccharides which play a key role in the induction of nodules on roots of legumes were unable to promote auxin- or cytokinin-independent cell division in tobacco protoplasts as claimed by Rohrig et al. (1995, 1996). The finding of van de Sande et al. (1996) that ENOD40 confers tolerance of high auxin concentration to wild-type tobacco protoplasts was also reinvestigated. The results of our investigations show that we were unable to reproduce the proliferation data presented in this study, which were obtained by counting tobacco protoplast-derived cells undergoing division. In total, none of the published data on phytohormone-independent division of tobacco cells could be reproduced. |
DOI: | 10.1046/j.1365-313X.1999.00404.x |
ISSN: | 0960-7412 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
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