Full metadata record
DC Field | Value | Language |
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dc.contributor.advisor | Aigars Reinis | - |
dc.contributor.author | Vladislavs Janšins | - |
dc.contributor.other | Medicīnas fakultāte | lv-LV |
dc.contributor.other | Faculty of Medicine | en-UK |
dc.date.accessioned | 2021-06-02T21:11:54Z | - |
dc.date.available | 2021-06-02T21:11:54Z | - |
dc.date.issued | 2021 | - |
dc.identifier.uri | https://dspace.rsu.lv/jspui/handle/123456789/4684 | - |
dc.description | Medicīna | lv-LV |
dc.description | Medicine | en-UK |
dc.description | Veselības aprūpe | lv-LV |
dc.description | Health Care | en-UK |
dc.description.abstract | Agrīna apendicīta diagnostika ir nepieciešama lai nodrošināt efektīvu bērnu ārstēšanu, tieši tāpēc ir nepieciešamība agrīniem biomarķieriem, agrīnai diagnoses uzstadīšanai. Mikrobioloģiskas nozimības noteikšana apendicīta etioloģijā palīdz sasniegt efektīvākus rezultātus konservativajā terapijā. Pētījuma mēŗķis ir noteikt visbiežāko mikroorganismu kurš izraisa akūto komplicēto (AkA) un akūtu nekomplicētu apendicītu (AnA), ka arī noteikt vai sēruma un urīna biomarķieriem vajadzētu būt iekļautiem agrīnajā diagnostikas algoritmā uz AkA un AnA. Pētījuma materiāli un metodes: pacienti vecumā no 7 līdz 17 gadiem bija iekļauti petijumā un sadalīti trijās grupās. Mikrobioloģiskās kultūras bija paņēmtas no appendix proksimālas un distālās daļas no visiem operetiem pacientiem, ka arī AkA gadijumā bija paņemta arī iztriepe no peritoneālā dobuma. Visi paraugi tika uzsieti uz asins agāra (aerobi un anaerobi), MacConkey agāra un mannitola sāls agāra stafilokoku izolācijai. Izolētas tīrkultūras bija identificētas pēc bioķimiskām īpašībām izmantojot VITEK2 iekartu. Statistiskā analīze bija izdarīta izmantojot Microsoft Excel 2016 un STATGRAPHICS Centurion 18. Pētijuma rezultāti: augstākais LRG-1 līmenis bija 22.7ng/mL kontroles grupā. Augstākais NGAL līmenis bija AkA grupā: serumā 169.1ng/mL, 137.0ng/mL, 92.5ng/mL un urinā 4.6ng/mL, 9.8ng/mL, 3.3ng/mL. Augstākais IL-6 līmenis bija AkA grupā: serumā 119.3pg/mL, 24.0pg/mL, 13.0pg/mL un urinā 15.6pg/mL, 37.5 pg/mL un 2.1pg/mL. Etioloģiskais spektrs AkA grupā iekļāva: 15 paraugus ar E.coli, 7 ar P.aeruginosa, 2 ar S.paucimobilis un citus. 26 peritonealās iztriepes paraugi iekļāva: 11 ar E.coli, 2 ar A.Baumanii un citus. Etioloģiskais spektrs AnA grupai iekļāva 10 paraugus ar E.coli, 2 ar C.braakii un citus. Šis aktīvs pētijums un vairāk pacientu tiks iekļāuti ar laiku. Secinājumā gribētos pateikt, ka uz doto brīdi visizplātitākais apendicīta ierosinatājs ir E.coli. Uz doto brīdi nav pārliecinošu pierādījumu, lai atšķirtu mikrobiotu no katras apendikālās anatomiskās vietas, kā arī tās saistību ar peritoneālo mikrobiotu. Abos apendicīta tipos LRG-1 produkcija bija zemāka nekā kontroles grupā, bet pēc operācijas biomarķiera līmenis atgriezās tuvu normai. NGAL iestašanās dienā bija paaugstināts apendicīta pacientu grupā, salidzīnot ar kontroles grupu. Sēruma LRG un urīna NGAL līmeņi nevarēja precīzi determinēt aktīvi notiekošo infekcijas procesu. Tomēr urīna IL-6 iestašanās dienā varētu tikt izmantots kā diagnostisks marķieris. | lv-LV |
dc.description.abstract | Early diagnostics of acute appendicitis is essential to provide effective treatment for children, and therefore, there is demand for early, accurate predictive appendicitis biomarkers. The knowledge of microbial role in etiology of appendicitis would help to achieve more effective conservative therapy. The aim of this study is to determine the most common microorganism that causes acute complicated (AcA) and uncomplicated appendicitis (AnA), as well as to determine whether serum and urine biomarkers should be included in the early diagnostic algorithm of AcA and AnA. Materials and Methods of the study: patients aged 7-17 were included and divided into three groups. Microbiological cultures were taken from the appendiceal proximal and distal ends from all operated appendicitis cases; in addition, cultures from the peritoneal cavity were examined in AcA cases. All the examples were examined using blood agar (aerobic and anaerobic), MacConkey agar, and mannitol salt agar for staphylococcal isolation. The isolated pure cultures were identified by biochemical properties using a VITEK2 analyzer. Statistical analysis was performed using Microsoft Excel 2016 and STATGRAPHICS Centurion 18. The results of the study: the highest level of LRG-1 was 22.7ng/mL in control group. The highest level of NGAL was in AcA group: serum 169.1ng/mL, 137.0ng/mL, 92.5ng/mL, and in urine 4.6ng/mL, 9.8ng/mL, 3.3ng/mL.The highest level of IL-6 was in AcA group: in serum 119.3pg/mL, 24.0pg/mL, 13.0pg/mL, and in urine 15.6pg/mL, 37.5pg/mL, and 2.1pg/mL. The etiological spectrum of AcA group include: fifteen with E.coli, seven with P.aeruginosa, two with S.paucimobilis and others. 26 peritoneal wall cultures revealed 11 with E.coli, two with A.baumanii and others. 17 culture results of AnA group revealed ten cases with E.coli, two with C.braakii and others. This is an ongoing study, in which more participants will be admitted. In conclusion I would like to say that currently, the most common etiological result from the bacterial cultures is E.coli. There is no conclusive evidence to differentiate the microbiota from each appendiceal anatomical location as well as its relation to the peritoneal microbiota. In both types of appendicitis, LRG-1 production was lower than in control samples, but after the operation, their levels approached closer to normal. NGAL on day-0 also is increased in appendicitis when compared to the control group. The levels of serum LRG and urine NGAL could not precisely determine the active infectious process till now. However, urine IL-6 at day-0 could possibly be used as a diagnostic marker. | en-UK |
dc.language.iso | en-UK | - |
dc.publisher | Rīgas Stradiņa universitāte | lv-LV |
dc.publisher | Rīga Stradiņš University | en-UK |
dc.rights | info:eu-repo/semantics/restrictedAccess | - |
dc.subject | Imunoloģija | lv-LV |
dc.subject | mikrobioloģija | lv-LV |
dc.subject | apendicīts | lv-LV |
dc.subject | pediatrija | lv-LV |
dc.subject | biomarķieri | lv-LV |
dc.subject | Immunology | en-UK |
dc.subject | microbiology | en-UK |
dc.subject | appendicitis | en-UK |
dc.subject | pediatrics | en-UK |
dc.subject | biomarkers. | en-UK |
dc.title | The ethiological spectrum and level of biomarkers in urine and serum in patients with acute complicated and uncomplicated | en-UK |
dc.title.alternative | Biomarķieru etioloģiskais spektrs un līmenis urīnā un serumā pacientiem ar akūtu komplicētu un nekomplicētu apendicītu | lv-LV |
dc.type | info:eu-repo/semantics/other | en-UK |
Appears in Collections: | Studējošo pētnieciskie darbi |
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