Full metadata record
DC Field | Value | Language |
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dc.contributor.author | Sokolovska, Lība | - |
dc.contributor.author | Sultanova, Alina | - |
dc.contributor.author | Čistjakovs, Maksims | - |
dc.contributor.author | Cunskis, Egils | - |
dc.contributor.author | Murovska, Modra | - |
dc.date.accessioned | 2021-01-13T13:04:18Z | - |
dc.date.available | 2021-01-13T13:04:18Z | - |
dc.date.issued | 2019 | - |
dc.identifier.uri | https://dspace.rsu.lv/jspui/handle/123456789/2896 | - |
dc.description.abstract | The aim of this study was to investigate the possibility of using monocytes/macrophages as mediators in human herpesvirus-6 (HHV-6) infection of thyroid gland tissues in autoimmune thyroiditis (AIT). Seventy-three AIT patients were enrolled in this study. The control group consisted of 80 blood donors. Monocyte/macrophage isolation for AIT patient samples was performed by adherence. HHV-6 was detected in peripheral blood mononuclear cell (PBMC) DNA samples using nested polymerase chain reaction (nPCR). Gene expression of HHV-6 active infection marker (U79/80) and chemokine receptors (U12, U51) in patient monocyte/macrophage samples and blood donor PBMC samples was detected using reverse-transcription PCR. HHV-6 viral load was detected by using quantitative-PCR technique. The HHV-6 genomic sequence was found significantly more frequently among AIT patient than control group samples. Markers of active infection were found in 8 AIT patient monocyte/macrophage samples (11%) and in none of control group PBMC samples. HHV-6 U51 mRNA expression was detected only in AIT patient samples (2/24 previously positive for HHV-6). Since HHV-6 genomic sequences were found significantly more frequently in AIT patient samples and active infection markers were found in patient monocytes/macrophages, our results suggest that monocytes/macrophages may be used by HHV-6 as mediators for thyroid gland infection. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Proceedings of the Latvian Academy of Sciences. Section B. Natural, Exact, and Applied Sciences. | Volume 73: Issue 2; DOI: https://doi.org/10.2478/prolas-2019-0018 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.subject | HHV-6 | en_US |
dc.subject | autoimmunity | en_US |
dc.subject | thyroid gland | en_US |
dc.title | Monocytes/Macrophages Act as Mediators for Human Herpesvirus-6 Infection of Thyroid Gland in Patients with Autoimmune Thyroiditis | en_US |
dc.type | Article | en_US |
Appears in Collections: | Publications |
Files in This Item:
File | Description | Size | Format | |
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MonocytesMacrophages Act as Mediators for Human Herpesvirus-6 Infection of Thyroid Gland in Patients with Autoimmune Thyroiditis.pdf | 275.01 kB | Adobe PDF | View/Open |
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