Full metadata record
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Groma, Valerija | - |
dc.contributor.author | Tarasovs, Mihails | - |
dc.contributor.author | Skuja, Sandra | - |
dc.contributor.author | Semenistaja, Sofija | - |
dc.contributor.author | Nora-Krukle, Zaiga | - |
dc.contributor.author | Svirskis, Simons | - |
dc.contributor.author | Murovska, Modra | - |
dc.date.accessioned | 2021-01-13T12:34:05Z | - |
dc.date.available | 2021-01-13T12:34:05Z | - |
dc.date.issued | 2020 | - |
dc.identifier.uri | https://dspace.rsu.lv/jspui/handle/123456789/2894 | - |
dc.description.abstract | A direct association between joint inflammation and the progression of osteoarthritis (OA) has been proposed, and synovitis is considered a powerful driver of the disease. Among infections implicated in the development of joint disease, human herpesvirus 7 (HHV-7) infection remains poorly characterized. Therefore, we assessed synovitis in OA patients; determined the occurrence and distribution of the HHV-7 antigen within the synovial membrane of OA-affected subjects; and correlated plasma levels of the pro-inflammatory cytokines tumor necrosis factor (TNF), interleukin-6 (IL-6), and TNF expressed locally within lesioned synovial tissues with HHV-7 observations, suggesting differences in persistent latent and active infection. Synovial HHV-7, CD4, CD68, and TNF antigens were detected immunohistochemically. The plasma levels of TNF and IL-6 were measured by an enzyme-linked immunosorbent assay. Our findings confirm the presence of persistent HHV-7 infection in 81.5% and reactivation in 20.5% of patients. In 35.2% of patients, virus-specific DNA was extracted from synovial membrane tissue samples. We evidenced the absence of histopathologically detectable synovitis and low-grade changes in the majority of OA patients enrolled in the study, in both HHV-7 PCR+ and HHV-7 PCR‒ groups. The number of synovial CD4-positive cells in the HHV-7 polymerase chain reaction (PCR)+ group was significantly higher than that in the HHV-7 PCR‒ group. CD4- and CD68-positive cells were differently distributed in both HHV-7 PCR+ and HHV-7 PCR‒ groups, as well as in latent and active HHV-7 infection. The number of TNF+ and HHV-7+ lymphocytes, as well as HHV-7+ vascular endothelial cells, was strongly correlated. Vascular endothelial cells, especially in the case of infection reactivation, appeared vulnerable. The balance between virus latency and reactivation is a long-term relationship between the host and infectious agent, and the immune system appears to be involved in displaying overreaction when a shift in the established equilibrium develops. | en_US |
dc.language.iso | en | en_US |
dc.relation.ispartof | Int. J. Mol. Sci. 2020, 21(17), 6004; https://doi.org/10.3390/ijms21176004 | en_US |
dc.rights | info:eu-repo/semantics/openAccess | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.subject | osteoarthritis | en_US |
dc.subject | synovium | en_US |
dc.subject | cytokines | en_US |
dc.subject | HHV-7 | en_US |
dc.subject | PCR | en_US |
dc.subject | ELISA | en_US |
dc.subject | immunohistochemistry | en_US |
dc.title | Inflammatory Cytokine-Producing Cells and Inflammation Markers in the Synovium of Osteoarthritis Patients Evidenced in Human Herpesvirus 7 Infection | en_US |
dc.type | Article | en_US |
Appears in Collections: | Publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
Inflammatory Cytokine-Producing Cells and Inflammation Markers in the Synovium of Osteoarthritis Patients Evidenced in Human Herpesvirus 7 Infection.pdf | 3.61 MB | Adobe PDF | View/Open |
This item is licensed under a Creative Commons License