Please use this identifier to cite or link to this item: 10.3390/cells13141208
Title: HSV-1 and Cellular miRNAs in CSF-Derived Exosomes as Diagnostically Relevant Biomarkers for Neuroinflammation
Authors: Scheiber, Christian
Klein, Hans C.
Schneider, Julian M.
Schulz, Tanja
Bechter, Karl
Tumani, Hayrettin
Kapapa, Thomas
Flinkman, Dani
Coffey, Eleanor
Ross, Duncan
Čistjakovs, Maksims
Nora-Krūkle, Zaiga
Bortolotti, Daria
Rizzo, Roberta
Murovska, Modra
Schneider, E. Marion
Institute of Microbiology and Virology
Keywords: CSF exosomes;encephalitis;HSV-1;IL-8;low-grade inflammation;miRNA;neuronal damage;NfL;oxidative stress;psychiatric disease;traumatic brain injury;1.6 Biological sciences;3.1 Basic medicine;3.2 Clinical medicine;1.1. Scientific article indexed in Web of Science and/or Scopus database;General Biochemistry,Genetics and Molecular Biology
Issue Date: Jul-2024
Citation: Scheiber , C , Klein , H C , Schneider , J M , Schulz , T , Bechter , K , Tumani , H , Kapapa , T , Flinkman , D , Coffey , E , Ross , D , Čistjakovs , M , Nora-Krūkle , Z , Bortolotti , D , Rizzo , R , Murovska , M & Schneider , E M 2024 , ' HSV-1 and Cellular miRNAs in CSF-Derived Exosomes as Diagnostically Relevant Biomarkers for Neuroinflammation ' , Cells , vol. 13 , no. 14 , 1208 , pp. 1-20 . https://doi.org/10.3390/cells13141208
Abstract: Virus-associated chronic inflammation may contribute to autoimmunity in a number of diseases. In the brain, autoimmune encephalitis appears related to fluctuating reactivation states of neurotropic viruses. In addition, viral miRNAs and proteins can be transmitted via exosomes, which constitute novel but highly relevant mediators of cellular communication. The current study questioned the role of HSV-1-encoded and host-derived miRNAs in cerebrospinal fluid (CSF)-derived exosomes, enriched from stress-induced neuroinflammatory diseases, mainly subarachnoid hemorrhage (SAH), psychiatric disorders (AF and SZ), and various other neuroinflammatory diseases. The results were compared with CSF exosomes from control donors devoid of any neuroinflammatory pathology. Serology proved positive, but variable immunity against herpesviruses in the majority of patients, except controls. Selective ultrastructural examinations identified distinct, herpesvirus-like particles in CSF-derived lymphocytes and monocytes. The likely release of extracellular vesicles and exosomes was most frequently observed from CSF monocytes. The exosomes released were structurally similar to highly purified stem-cell-derived exosomes. Exosomal RNA was quantified for HSV-1-derived miR-H2-3p, miR-H3-3p, miR-H4-3p, miR-H4-5p, miR-H6-3p, miR-H27 and host-derived miR-21-5p, miR-146a-5p, miR-155-5p, and miR-138-5p and correlated with the oxidative stress chemokine IL-8 and the axonal damage marker neurofilament light chain (NfL). Replication-associated miR-H27 correlated with neuronal damage marker NfL, and cell-derived miR-155-5p correlated with oxidative stress marker IL-8. Elevated miR-138-5p targeting HSV-1 latency-associated ICP0 inversely correlated with lower HSV-1 antibodies in CSF. In summary, miR-H27 and miR-155-5p may constitute neuroinflammatory markers for delineating frequent and fluctuating HSV-1 replication and NfL-related axonal damage in addition to the oxidative stress cytokine IL-8 in the brain. Tentatively, HSV-1 remains a relevant pathogen conditioning autoimmune processes and a psychiatric clinical phenotype.
Description: Publisher Copyright: © 2024 by the authors.
DOI: 10.3390/cells13141208
ISSN: 2073-4409
Appears in Collections:Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure

Files in This Item:
File SizeFormat 
HSV_1_and_Cellular_mi_RNAs.pdf6.16 MBAdobe PDFView/Openopen_acces_unlocked


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.