Please use this identifier to cite or link to this item:
10.1371/journal.pone.0262968
Title: | OverFlap PCR : A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias |
Authors: | Linars, Artis Silamikelis, Ivars Gudra, Dita Roga, Ance Fridmanis, Davids |
Keywords: | 2.8 Environmental biotechnology;1.6 Biological sciences;3.4 Medical biotechnology;1.1. Scientific article indexed in Web of Science and/or Scopus database;General |
Issue Date: | Aug-2022 |
Citation: | Linars , A , Silamikelis , I , Gudra , D , Roga , A & Fridmanis , D 2022 , ' OverFlap PCR : A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias ' , PloS one , vol. 17 , e0262968 . https://doi.org/10.1371/journal.pone.0262968 |
Abstract: | Over the decades, practical biotechnology researchers have aimed to improve naturally occurring proteins and create novel ones. It is widely recognized that coupling protein sequence randomization with various effect screening methodologies is one of the most powerful techniques for quickly, efficiently, and purposefully acquiring these desired improvements. Over the years, considerable advancements have been made in this field. However, developing PCR-based or template-guided methodologies has been hampered by resultant template sequence biases. Here, we present a novel whole plasmid amplification-based approach, which we named OverFlap PCR, for randomizing virtually any region of plasmid DNA without introducing a template sequence bias. |
Description: | Publisher Copyright: © 2022 Linars et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0262968 |
ISSN: | 1932-6203 |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
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