Please use this identifier to cite or link to this item: 10.1016/j.dib.2023.108890
Title: Data on cardiac and vascular functionality in ex vivo and in vivo models following acute administration of trimethylamine N-oxide
Authors: Videja, Melita
Vilskersts, Reinis
Sevostjanovs, Eduards
Liepinsh, Edgars
Dambrova, Maija
Faculty of Pharmacy
Keywords: Cardiovascular functionality;Endothelial function;Energy substrate metabolism;Myocardial infarction;Trimethylamine N-oxide;3.1 Basic medicine;1.1. Scientific article indexed in Web of Science and/or Scopus database;General
Issue Date: Feb-2023
Citation: Videja , M , Vilskersts , R , Sevostjanovs , E , Liepinsh , E & Dambrova , M 2023 , ' Data on cardiac and vascular functionality in ex vivo and in vivo models following acute administration of trimethylamine N-oxide ' , Data in Brief , vol. 46 , 108890 . https://doi.org/10.1016/j.dib.2023.108890
Abstract: This dataset describes in detail the outcomes of acute trimethylamine N-oxide (TMAO) administration on cardiac, vascular and mitochondrial functionality in ex vivo and in vivo models. The accumulation of TMAO in target tissues was assessed after performing heart perfusion or by incubating aortic tissue in a solution containing TMAO. To evaluate the impact of TMAO on mitochondrial function, the aortic rings and heart homogenates of Wistar rats were incubated in a solution containing [9,10- 3H] palmitate (5 µCi/ml) or D-[U- 14C] glucose (0.625 µCi/ml) in the presence or absence of TMAO with subsequent measurement of substrate oxidation and uptake. The effects of TMAO on the vascular reactivity of isolated conductance and resistance vessels were tested by measuring their response to acetylcholine and sodium nitroprusside. The impact of elevated TMAO levels on cardiac function and infarct size caused by ischemia-reperfusion injury was evaluated in Langendorff perfused heart model. Normal and forced heart functioning was analyzed by echocardiography in CD-1 mouse acute cardiac stress model induced by isoproterenol (10 µg/mouse) upon single and 7 repeated daily administrations of TMAO (120 mg/kg). The data presented in the manuscript provide valuable information on measurements performed under conditions of acutely elevated TMAO levels in experimental models of cardiac and vascular function and energy metabolism. Furthermore, the data have high reuse potential as they could be applied in the planning of future in vitro, ex vivo, and in vivo studies addressing the molecular mechanisms targeted by elevated levels of TMAO.
Description: Funding Information: This study was performed within the Latvian Council of Science project “Trimethylamine N-oxide as a link between unhealthy diet and cardiometabolic risks” No. Izp-2018/1-0081, supervised by M.D.; and M.V. received funding from the European Social Fund and the state budget within the project No. 8.2.2.0/20/I/004 “Support for involving doctoral students in scientific research and studies”. Publisher Copyright: © 2023 The Author(s)
DOI: 10.1016/j.dib.2023.108890
ISSN: 2352-3409
Appears in Collections:Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure

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