Local defence system in healthy lungs
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Date
2023
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Rīgas Stradiņa universitāte
Rīga Stradiņš University
Rīga Stradiņš University
Abstract
Respiratorā sistēm ir viens no galvenajiem infekcijas ieejas vārtiem. Šī darba mērķis bija salīdzināt gļotādas specifisko iekaisumu stimulējošo faktoru un kopējo pretiekaisuma faktoru sintēzi veselos plaušu audos no ontogenētiskā viedokļa. Materiāli un metodes: Veselie plaušu audi tika iegūti no 15 pacientiem (3 sievietes un 12 vīrieši) vecumā no 18 līdz 86 gadiem. Imūnhistoķīmijas metode tika izmantota, lai noteikt cilvēka β defensīnu 2 (HBD-2), cilvēka β defensīnu 3 (HBD-3), cilvēka β defensīnu 4 (HBD-4), katelicidīnu (LL-37) un interleikīnu 17A (IL-17A). Rezultāti: Visi iegūtie plaušu audu paraugi saturēja gan bronhiālo epitēliju, gan plaušu parenhimālos audus, kā arī nevienā no audu paraugiem netika novērotas iekaisuma pazīmes. Pētījuma ietvaros tika novērotas statistiski nozīmīgas atšķirības dažādu faktoru ekspresijā visās pētamajās plaušu audu lokalizācijās. Secinājumi: Visas veselu plaušu struktūras, īpaši skrimšļaudi, alveolārais epitēlijs un alveolārie makrofāgi, kalpo par galveno sintēzes avotu pretmikrobu proteīniem un IL-17A. Skrimslis parādīja augstu funkcionālo plasticitāti, iekļaujot izteiktu pretmikrobu aktivitāti un piedalīšanos plaušu udu lokālajā aizsardzībā. Savstarpēji atkarīgas antimikrobiālo faktoru sintēzes izmaiņas dažādās plaušu audu lokalizācijās norāda par savstarpēju sinerģistisku faktoru sadarbību ar mērķi nodrošināt adekvātu vezels plaušu aizsardzību.
Background: The respiratory system is one of the main entrance gates for infection. The aim of this work was to compare the appearance of specific mucosal pro-inflammatory and common anti-microbial defence factors in healthy lung tissue, from an ontogenetic point of view. Materials and methods: Healthy lung tissues were collected from 15 patients (three females and 12 males) in the age range from 18 to 86. Immunohistochemistry to human β defensin 2 (HBD-2), human β defensin 3 (HBD-3), human β defensin 4 (HBD-4), cathelicidine (LL-37) and interleukine 17A (IL-17A) were performed. Results: The lung tissue material contained bronchial and lung parenchyma material in which no histological changes, connected with the inflammatory process, were detected. During the study, various statistically significant differences were detected in immunoreactive expression between different factors in all lung tissue structures. Conclusion: All healthy lung structures, but especially the cartilage, alveolar epithelium and the alveolar macrophages, are the main locations for the baseline synthesis of antimicrobial proteins and IL-17A. Cartilage shows high functional plasticity of this structure, including significant antimicrobial activity and participation in local lung protection response. Interrelated changes between antimicrobial proteins in different tissue confirm baseline synergistical cooperation of all these factors in healthy lung host defence.
Background: The respiratory system is one of the main entrance gates for infection. The aim of this work was to compare the appearance of specific mucosal pro-inflammatory and common anti-microbial defence factors in healthy lung tissue, from an ontogenetic point of view. Materials and methods: Healthy lung tissues were collected from 15 patients (three females and 12 males) in the age range from 18 to 86. Immunohistochemistry to human β defensin 2 (HBD-2), human β defensin 3 (HBD-3), human β defensin 4 (HBD-4), cathelicidine (LL-37) and interleukine 17A (IL-17A) were performed. Results: The lung tissue material contained bronchial and lung parenchyma material in which no histological changes, connected with the inflammatory process, were detected. During the study, various statistically significant differences were detected in immunoreactive expression between different factors in all lung tissue structures. Conclusion: All healthy lung structures, but especially the cartilage, alveolar epithelium and the alveolar macrophages, are the main locations for the baseline synthesis of antimicrobial proteins and IL-17A. Cartilage shows high functional plasticity of this structure, including significant antimicrobial activity and participation in local lung protection response. Interrelated changes between antimicrobial proteins in different tissue confirm baseline synergistical cooperation of all these factors in healthy lung host defence.
Description
Medicīna
Medicine
Veselības aprūpe
Health Care
Medicine
Veselības aprūpe
Health Care
Keywords
veselas plaušas; faktori; imunitāte; respiratorā sistēma; lokālā aizsardzība, healthy lungs; factors; immunity; respiratory system; local defence