Distribution and Appearance of Ki-67, IL-1α, IL-10, and PGP 9.5 in Reinkeʼs Oedema- Affected Larynx Tissue Compared with Control Tissue
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Date
2023
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Rīgas Stradiņa universitāte
Rīga Stradiņš University
Rīga Stradiņš University
Abstract
Smēķēšana, laringofaringālais reflukss un balss saišu pārmērīga noslogošana var veicināt Reinkes tūskas attīstību, izraisot balss saišu disfunkcijas un traumas. Pētījuma mērķis: Ki-67 (Ki-67), interleikīna-10 (IL-10), interleikīna-1 alfa (IL-1α) un proteīna gēna produkta 9.5 (PGP 9.5) imūnhistoķīmiskā noteikšana Reinkes tūskas skartos balsenes audos un kontroles balsenes audos. Metodes: Tika veikta pacientu un kontroles grupas balsenes audu histoloģiskā un imūnhistoķīmiskā analīze. Faktori Ki-67, IL-10, IL-1α un PGP 9.5 tika noteikti, pielietojot Biotīna streptavidīna imūnhistoķīmijas metodi. Imūnreaktīvo struktūru lokālās izplatības novērtēšana tika veikta, izmantojot puskvantatīvo skaitīšanas metodi. Statistiskajā analīzē tika aprēķināts Manna-Vitnija U tests un Spīrmena ranga korelācijas koeficients. Rezultāti: Pacientu grupā tika konstatēta zema pozitīva korelācija starp IL-1α epitēlija un subepitēlija imūnreaktīvajām struktūrā. Manna-Vitnija U tests atklāja statistiski nozīmīgas atšķirības starp pacientu un kontroles grupu.
Secinājumi: Pacientu audos tika novērota pastiprināta epitēlija proliferācija. Ievērojams IL-10 imūnhistoķīmiski pozitīvo struktūru skaita pieaugums Reinkes tūskas skartos balsenes audos liecina par dominējošu pretiekaisuma audu reakciju. Palielinātais imūnhistoķīmiski pozitīvo IL-1α struktūru skaits balsenes epitēlijā un subepitēlijā pacientu grupā ir saistīts ar iekaisumu, proliferāciju un audu remodelāciju. PGP 9.5 imūnhistoķīmiski pozitīvo struktūru skaita palielināšanās pacientu grupā ir saistīts ar Reinkes tūskas morfopatoģenēzi.
Smoking, laryngopharyngeal reflux, and vocal fold abuse can promote the development of Reinke’s oedema, leading to vocal fold dysfunction and injury. The aim of the work was to investigate the appearance and distribution of proliferation marker Ki-67 (Ki-67), interleukin-10 (IL-10), interleukin-1 alpha (IL-1α), and protein gene peptide 9.5 (PGP 9.5) in Reinke’s oedema-affected larynx tissue. Methods: A routine histological and immunohistochemical Reinke’s oedema and control group patient analysis was conducted. We used the biotin–streptavidin biochemical method to detect Ki-67, IL-10, IL-1α, and PGP 9.5 The semiquantitative grading method was used to evaluate immunoreactive cells’ appearance and local distribution. A Mann–Whitney U test and Spearman’s rank coefficient were performed. Results: A low positive correlation between IL-1α epithelial and subepithelial immunoreactive cells in the patient group was found. Mann–Whitney U tests revealed significant patient and control group immunoreactive marker differences. All examined markers showed a higher number of immunoreactive structures in the patient group. Conclusions: Intensive proliferation of the surface epithelium was observed in patient tissues. The notable increase in IL-10 positive structures indicates the dominant anti-inflammatory tissue response. An increased number of IL-1α structures in the larynx epithelium and subepithelium in the patient group is linked to inflammation, proliferation, and tissue remodelling. The PGP 9.5 expression increase is involved in the morphopathogenesis of Reinke’s oedema.
Smoking, laryngopharyngeal reflux, and vocal fold abuse can promote the development of Reinke’s oedema, leading to vocal fold dysfunction and injury. The aim of the work was to investigate the appearance and distribution of proliferation marker Ki-67 (Ki-67), interleukin-10 (IL-10), interleukin-1 alpha (IL-1α), and protein gene peptide 9.5 (PGP 9.5) in Reinke’s oedema-affected larynx tissue. Methods: A routine histological and immunohistochemical Reinke’s oedema and control group patient analysis was conducted. We used the biotin–streptavidin biochemical method to detect Ki-67, IL-10, IL-1α, and PGP 9.5 The semiquantitative grading method was used to evaluate immunoreactive cells’ appearance and local distribution. A Mann–Whitney U test and Spearman’s rank coefficient were performed. Results: A low positive correlation between IL-1α epithelial and subepithelial immunoreactive cells in the patient group was found. Mann–Whitney U tests revealed significant patient and control group immunoreactive marker differences. All examined markers showed a higher number of immunoreactive structures in the patient group. Conclusions: Intensive proliferation of the surface epithelium was observed in patient tissues. The notable increase in IL-10 positive structures indicates the dominant anti-inflammatory tissue response. An increased number of IL-1α structures in the larynx epithelium and subepithelium in the patient group is linked to inflammation, proliferation, and tissue remodelling. The PGP 9.5 expression increase is involved in the morphopathogenesis of Reinke’s oedema.
Description
Medicīna
Medicine
Veselības aprūpe
Health Care
Medicine
Veselības aprūpe
Health Care
Keywords
Reinkes tūska; IL-10; IL-1α; Ki-67; PGP 9.5; balsene, Reinke’s oedema; IL-10; IL-1α; Ki-67; PGP 9.5; larynx