Gastric carcinomas with stromal b7-h3 expression have lower intratumoural cd8+ t cell density
No Thumbnail Available
Date
2021-02-02
Journal Title
Journal ISSN
Volume Title
Publisher
Abstract
CD8+ T cells are the main effector cells of anti-cancer immune response that can be regulated by various costimulatory and coinhibitory molecules, including members of the B7 family. B7 homolog 3 (B7-H3) appears as a promising marker for immunotherapy; however, its significance in gastric cancer (GC) is unclear yet. We evaluated the spatial distribution of CD8+ T cells in relation to the expression of B7-H3 by double immunohistochemical staining. The level of B7-H3 intensity was scored manually (0-3) and dichotomized into B7-H3-low and B7-H3-high groups. The distribution and density of CD8+ T cells was analysed using whole slide digital imaging. B7-H3 was expressed mainly in the stromal compartment of GC (n = 73, 76% of all cases). Tumours with high expression of B7-H3 showed larger spatial differences of CD8+ T cells (86.4/mm2 in tumour centre vs. 414.9/mm2 in invasive front) when compared to B7-H3-low group (157.7/mm2 vs. 218.7/mm2, respectively) (p < 0.001). This study provides insight into the expression pattern of B7-H3 in GC of Western origin. In GCs with higher level of B7-H3 expression, CD8+ T cells were spatially suppressed in the tumour centre suggesting that B7-H3 might be involved in tumour escape mechanisms from the immune response.
Description
Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
Keywords
B7-H3, CD8-Positive T-Lymphocytes, Gastric cancer, Immune checkpoint, Immune evasion, Immunohistochemistry, Tumour escape, Tumour microenvironment, 3.1 Basic medicine, 1.1. Scientific article indexed in Web of Science and/or Scopus database, Catalysis, Molecular Biology, Spectroscopy, Computer Science Applications, Physical and Theoretical Chemistry, Organic Chemistry, Inorganic Chemistry, SDG 3 - Good Health and Well-being
Citation
Ulase, D, Behrens, H M, Krüger, S, Zeissig, S & Röcken, C 2021, 'Gastric carcinomas with stromal b7-h3 expression have lower intratumoural cd8+ t cell density', International Journal of Molecular Sciences, vol. 22, no. 4, 2129, pp. 1-8. https://doi.org/10.3390/ijms22042129