Ārstniecības augu ekstraktu iekapsulēšanas iespējas etosomās - metodikas izstrāde un raksturojums.
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Date
2023
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Rīgas Stradiņa universitāte
Rīga Stradiņš University
Rīga Stradiņš University
Abstract
Nanodaļiņas un ārstniecības augi ir vieni no šī brīža aktuālākajiem pētniecības virzieniem. Kā viena, tā otra potenciāls strauji attīstās, un parādās iespējas tos daudzpusīgi izmantot, piemēram, zāļu piegādes sistēmās. Attiecībā uz transdermālu aktīvo vielu piegādes ceļu, etosomas ieņem būtisku lomu, balstoties uz to potenciālajām fizikāli ķīmiskajām īpašībām un aktīvo vielu iekapsulēšanas iespējām.
Pētnieciskā darba mērķis bija novērtēt 2 antioksidantiem bagātu ārstniecības augu Vaccinium corymbosum un Vaccinium myrtillus augļu ekstraktu iekapsulēšanas iespējas etosomās. Lai to paveiktu, tika izveidotas dažāda sastāva etosomas, izmantojot auksto metodi. Optimizētās etosomas tika raksturotas pēc vidējā diametra, polidispersitātes indeksa, zeta-potenciāla, izmantojot dinamiskās gaismas izkliedes metodi. Turklāt, procentuālā iekapsulēšanas efektivitāte tika noteikta, izmantojot ultracentrifugēšanu un augstas efektivitātes šķidruma hromatogrāfijas metodes. Tālāk ar etosomām tika veikta Furjē-transformācijas infrasarkanās spektroskopijas (FTIR) pētījumi.
Pētījuma ietvaros, tika izstrādātas un optimizētas divu dažādu sastāvu etosomas, kurās tika izmantots fosfatidilholīns ar un bez holesterola, pēc tam iekapsulējot liofilizētus melleņu ekstraktus. Negatīvi lādētajām ar melleņu ekstraktu pildītajām etosomām diametrs ir 200 – 300 nm, ar polidispersitātes indeksu ap 0,3, kas apstiprina monomodālu izmēru sadalījumu. Holesterola klātbūtne lielā mērā ir ietekmējusi etosomu kopējo diametru. Etosomas bija fizikāli stabilas uzglabāšanas laikā. Melleņu ekstrakta saturs abu veidu vezīkulās palika gandrīz tāds pats, proti, aptuveni 60%. FTIR pētījumi apstiprināja visu komponentu kopējo saderību iekapsulēto etosomu dispersijā.
Pētījumā iegūtie rezultāti sniedz iespēju etosomas virzīt tālākai izpētei transdermālai aktīvo vielu piegādei, piemēram, grūti dzīstošu brūču gadījumā.
Nanoparticles and medicinal plants are one of the most relevant research directions at the moment. The potential of one and the other is rapidly developing, and opportunities are emerging to use them versatiley, for example in drug delivery systems. Regarding the transdermal delivery route of active substances, ethosomes play an essential role based on their potential physiochemical properties and active substance encapsulation capabilities. The aim of the study was to evaluate the possibilities of encapsulation of fruit extracts of two antioxidant-rich medicinal plants Vaccinium corymbosum and Vaccinium myrtillus. In this regard, ethosomes along with different compositions have been produced via the cold method. The optimized ethosomes have been characterized in terms of average diameter, polydispersity index, zeta-potential using dynamic light scattering. Moreover, percentage encapsulation efficiency was monitored by ultracentrifugation and HPLC method. Further, Fourier-transform infrared spectroscopy (FTIR) studies have been conducted on the ethosomes. As part of the study, two different compositions of ethosomes were developed and optimized wherein, phosphatidylcholine has been employed with and without cholesterol, and the lyophilized blueberry extracts have been incorporated in the final formulation. The negativley charged blueberry loaded ethosomes have diameter 200 – 300 nm with polydispersity around 0,3 confirming monomodal dimensional distribution. The presence of cholesterol has largley influenced the overall diameter of the ethosomes. The ethosomes were physically stable during storage time. The blueberry content in both type of vesicles remained almost the same, that is around 60%. The FTIR studies confirmed the overall compatibility of all components in the final ethosomal dispersion. The results obtained in the study provides an opportunity to direct ethosomes to further research for transdermal delivery of active substances, for example, in the case of hard-to-heal wounds.
Nanoparticles and medicinal plants are one of the most relevant research directions at the moment. The potential of one and the other is rapidly developing, and opportunities are emerging to use them versatiley, for example in drug delivery systems. Regarding the transdermal delivery route of active substances, ethosomes play an essential role based on their potential physiochemical properties and active substance encapsulation capabilities. The aim of the study was to evaluate the possibilities of encapsulation of fruit extracts of two antioxidant-rich medicinal plants Vaccinium corymbosum and Vaccinium myrtillus. In this regard, ethosomes along with different compositions have been produced via the cold method. The optimized ethosomes have been characterized in terms of average diameter, polydispersity index, zeta-potential using dynamic light scattering. Moreover, percentage encapsulation efficiency was monitored by ultracentrifugation and HPLC method. Further, Fourier-transform infrared spectroscopy (FTIR) studies have been conducted on the ethosomes. As part of the study, two different compositions of ethosomes were developed and optimized wherein, phosphatidylcholine has been employed with and without cholesterol, and the lyophilized blueberry extracts have been incorporated in the final formulation. The negativley charged blueberry loaded ethosomes have diameter 200 – 300 nm with polydispersity around 0,3 confirming monomodal dimensional distribution. The presence of cholesterol has largley influenced the overall diameter of the ethosomes. The ethosomes were physically stable during storage time. The blueberry content in both type of vesicles remained almost the same, that is around 60%. The FTIR studies confirmed the overall compatibility of all components in the final ethosomal dispersion. The results obtained in the study provides an opportunity to direct ethosomes to further research for transdermal delivery of active substances, for example, in the case of hard-to-heal wounds.
Description
Farmācija
Pharmacy
Veselības aprūpe
Health Care
Pharmacy
Veselības aprūpe
Health Care
Keywords
Atslēgas vārdi: Etosomas, nanodaļiņas, Vaccinium corymbosum, Vaccinium myrtillus, ekstrakti, iekapsulēšanas efektivitāte., Keywords: Ethosomes, nanoparticles, Vaccinium corymbosum, Vaccinium myrtillus, extracts, encapsulation efficiency