Morphological Characteristics of Chronic Obstructive Pulmonary Disease (COPD) Affected Lung Tissue. Summary of the Doctoral Thesis

Simple item page
dc.contributor.advisorPilmane, Māra
dc.contributor.authorVitenberga-Verza, Zane
dc.date.accessioned2020-12-17T15:18:09Z
dc.date.available2020-12-17T15:18:09Z
dc.date.issued2020
dc.descriptionThe Doctoral Thesis was developed at the Department of Morphology, Institute of Anatomy and Anthropology, Rīga Stradiņš University, Latvia. Defence: at the public session of the Promotion Council of the Basic Sciences of Medicine, including Pharmacology on 28 December 2020 at 10.00 in Hippocrates Lecture Theatre, 16 Dzirciema Street, Rīga Stradiņš University and using the Zoom platform.
dc.description.abstractChronic obstructive pulmonary disease (COPD) is a potentially preventable and treatable disease characterized by progressive airway disorders. Under the influence of various risk factors, oxidative stress and chronic inflammation develop, which determines the formation and course of practically all other processes (for example, local fibrosis). Even with the maximal elimination of risk factors, inflammation in COPD persists and even continues. In general, COPD morphopathogenesis is characterized by oxidative stress, chronic inflammation, remodeling, as well as impaired antimicrobial and other locally realized tissue protective responses. The aim of the study was to determine and evaluate the morphological characteristics of bronchial tissues affected by COPD and analyze the tissue distribution of markers relevant to chronic inflammation, changes in tissue remodeling and local protective mechanisms. Also, these markers were evaluated in ontogenesis perspective. The study included 40 COPD and 49 relatively healthy control patients. Tissue material from COPD patients was collected during bronchoscopy. Tissue material from the control group was obtained by autopsy from individuals of different ages who died of accidents and from diseases not affecting respiratory system, as well as from / or during lung surgery. IL-1α (interleukin-1 alpha), IL-4 (interleukin-4), IL-6 (interleukin-6), IL-7 (interleukin-7), IL-8 (interleukin-8), IL-10 (interleukin-10), IL-12 (interleukin-12), TNF-α (tumour necrosis factor-alpha), TGF-β1 (transforming growth factor-beta 1), MMP-2 (matrix metalloproteinase-2), TIMP-2 (matrix metalloproteinase-2 tissue inhibitor), Hsp-70 (heat shock protein-70), hBD-2 (human beta defensin-2), hBD-3 (human beta defensin-3), hBD-4 ( human beta defensin-4) immunoreactive cell counts were determined by immunohistochemistry in eight different tissue groups and lung localizations in control and COPD patients, and also compared. The immunohistochemical findings were also analyzed in terms of ontogenesis or aging. In the lung tissues of the control group, we found a mild to moderate increase in the number of immunoreactive cells of all studied factors at different localizations, indicating low, persistent, continuous and adaptive “baseline” level for various factors. Lung tissue in COPD patients is characterized by variable and various localized changes with chronic inflammation and tissue remodeling including inflammatory cell infiltration, granulation tissue, thickened basement membrane, bronchial gland and smooth muscle hypeplasia and hypertrophy, also thickened vascular fibrosis, moreover, fibrosis and epithelial metaplasia. The most pronounced finding of the studied tissue factors was in bronchial epithelium, mucosal connective tissue and blood vessels of COPD patients, which proves the significant involvement and role of these structures in COPD morphopathogenesis. Overall, COPD morphopathogenesis is characterized by increased IL-1α, IL-4, IL-6, IL-8, TNF-α, IL-7, IL-10, IL-12, TGF-β1, MMP-2, TIMP-2, hBD-2 and hBD-3 immunoreactive cell counts, as well as reduced Hsp-70 and hBD-4-containing cell counts, indicating high activity of persistent complex cytokine pleiade, tissue remodeling and total tissue antimicrobial protection. Ontogenesis dependent changes, including in terms of aging, within the lungs of relatively healthy individuals are associated with mild inflammation, aging of immune system cells and disorder of immune system regulation. Age changes in the relatively healthy lungs are individual, even different for individuals in different age groups, as well as mostly characterized by a lack to maintain inflammation, also, reduced number of immune cells are found. In elderly COPD patients within ontogenesis, we detected an increase in the numbers of IL-1α, IL-4, IL-6, IL-10, IL-12, MMP-2, hBD-4 immunoreactive cells, but decreased numbers of IL-7, IL-8, TNF-α, TIMP-2, TGF-β1, Hsp-70, hBD-2, hBD-3 immunoreactive cells with age, as well as chronic bronchitis, epithelial metaplasia, granulation tissue and poorer functional parameters. In the case of COPD, aging is associated with the worsening of the disease, as well as persistence and altered remodeling of inflammatory cytokines otherwise characteristic of COPD.
dc.formatElectronic
dc.identifier.citationVitenberga-Verza, Z. 2020. Morphological Characteristics of Chronic Obstructive Pulmonary Disease (COPD) Affected Lung Tissue: Summary of the Doctoral Thesis: Sub-Sector – Histology and Cytology. Rīga: Rīga Stradiņš University. https://doi.org/10.25143/prom-rsu_2020-19-dts
dc.identifier.doihttps://doi.org/10.25143/prom-rsu_2020-19-dts
dc.identifier.other1-88
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/2837
dc.language.isoen_US
dc.publisherRīga Stradiņš University
dc.rightsinfo:eu-repo/semantics/openAccess
dc.rightsAttribution-NonCommercial 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc/4.0/*
dc.subjectSector – Basic Sciences of Medicine, including Pharmacology, Sub-Sector – Histology and Cytology
dc.subjectSummary of the Doctoral Thesis
dc.titleMorphological Characteristics of Chronic Obstructive Pulmonary Disease (COPD) Affected Lung Tissue. Summary of the Doctoral Thesis
dc.title.alternativeHroniskas obstruktīvas plaušu slimības (HOPS) skartu audu morfoloģisks raksturojums. Promocijas darba kopsavilkums
dc.typeinfo:eu-repo/semantics/other

Files

Original bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
2020-19_Vitenberga-Verza-Zane_DTS_IPD-1013.pdf
Size:
2.15 MB
Format:
Adobe Portable Document Format
Download

Collections

2020. gadā aizstāvētie promocijas darbi un kopsavilkumi