Browsing by Author "Pilmane, M."

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    Alternative for improving gut microbiota : Use of Jerusalem artichoke and probiotics in diet of weaned piglets
    (2014-03-01) Valdovska, Anda; Jemeljanovs, A.; Pilmane, M.; Zitare, I.; Konosonoka, I. H.; Lazdins, M.; Institute of Anatomy and Anthropology
    The aim of the study was to determine the effect of Jerusalem artichoke and probiotics on defence activity of intestinal cells of weaning pigs. One hundred eighty piglets (7 weeks old) were fed with basal feed supplemented with Jerusalem artichoke, Lactobacillus reuteri and Pediococcus pentosaceus. After 5 weeks, the piglets were slaughtered and the gastrointestinal contents and intestine samples were taken for analysis. Results demonstrated that in pigs fed basal diet with both probiotics and Jerusalem artichoke (5% of basal diet) (T3 group) had less (P<0.05) faecal Enterobacteriaceae microorganisms and coliforms and had more (P<0,05) faecal Lactobacillus than in pigs from other groups. Increase by 2% of Enterobacteriaceae and E.coli levels were seen only in control piglets (T1 group). E.coli O157 was found at the closing stage in the piglets fed basal diet with only Jerusalem artichoke powder (T2 group), but Salmonella enteritidis-only in T1 group. In jejunum of T2 group piglets, large deterioration of crypts, a moderate inflammation process and plasmocytes were seen, but in jejunum of T3 group piglets-branching of apical surface of villi, moderate degeneration and mitosis of enterocytes were observed. A moderate number of apoptotic cells in T2 group was found mainly in colon inflammation cells and plasmocytes, but for T3 group piglets-both in jejunum enterocytes and migrating cells. Our study indicated that β-defensin 2 and 3 expression in jejunum and colon segments were incresed in T1 and T2 groups. Findings suggest that feeding with probiotics and Jerusalem artichoke significantly improves the microbial contents, defence and regeneration processes in the intestine of pigs.
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    Changes in neuroendocrine elements in bronchial mucosa in chronic lung disease in adults
    (1995) Pilmane, M.; Luts, A.; Sundler, F.; Department of Morphology
    Background - It is not clear whether there is any association between metaplasia of the bronchial epithelium and changes in the distribution of neuroendocrine cells. This study examined, by immunohistological techniques, the distribution of neuroendocrine cells and juxtamucoscal nerve fibres in bronchial biopsies showing metaplastic changes. Methods - Bronchial biopsies from 12 subjects with epithelial metaplasia associated with bronchiectasis and diffuse pulmonary fibrosis were examined by conventional light microscopy and immunohistological techniques for protein gene product 9.5 (PGP), chromogranin A and B (CAB), serotonin, vasoactive intestinal peptide (VIP), substance P (SP), calcitonin generelated peptide (CGRP), calcitonin (CT), and gastrin releasing peptide (GRP). Results - Regions of non-metaplastic epithelium contained numerous PGP and serotonin immunoreactive cells. Subpopulations of these cells displayed CAB, CGRP, CT, and GRP immunoreactivity. Metaplastic epithelium contained only a few weakly stained PGP, serotonin, CAB, GRP, CT and CGRP immunoreactive cells in six cases. Metaplastic epithelium was characterised by a high number of CABcontaining cells in six cases and in these biopsies prominent PGP-contaiIiing nerve bundles were seen in the subepithelial layer beneath the metaplastic epithelium. Conclusions - The distribution patterns of neuroendocrine cells and neuronal elements vary between areas of normal and metaplastic epithelium and within areas of metaplastic epithelium. Neuronal hyperplasia was associated with an increase in the number of CAB-containing cells within the metaplastic epithelium.
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    The expression of cytokines and beta -defensin 2,-3,-4 in rabbit bone tissue after hydroxyapatite (HAp), alpha-Tricalcium phosphate (alpha-TCP) and polymethylmethacrylate (PMMA) implantation
    (2012) Vamze, J.; Pilmane, M.; Skagers, A.; Rīga Stradiņš University; Institute of Anatomy and Anthropology
    Bone loss induced by inflammation is one of the complications after biomaterial implantation. There is no much data on expression of cytokines and defensins into the bone tissue around the implants in literature. The aim of this work was to investigate the distribution and appearance of interleukin (IL)-1, IL-6, IL-8, IL10 and β – defensin (BD)-2, BD-3, BD-4 after the implantation of different biomaterials. Bone developing zones, signs of bone-implant contact and low expression of pro-inflammatory cytokine IL-1, IL-6 and anti-inflammatory cytokine IL-10 in experimental tissue with pure HAp and unburned HAp implants indicate a potential advantage of this material in terms of its biocompatibility over the other materials used in our study.
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    The expression of cytokines and β -defensin 2, - 3, -4 in rabbit bone tissue after hydroxyapatite (HAp), α- Tricalcium phosphate (α-TCP) and polymethylmethacrylate (PMMA) implantation
    (2012) Vamze, J.; Pilmane, M.; Skagers, A.; Rīga Stradiņš University; Institute of Anatomy and Anthropology
    Bone loss induced by inflammation is one of the complications after biomaterial implantation. There is no much data on expression of cytokines and defensins into the bone tissue around the implants in literature. The aim of this work was to investigate the distribution and appearance of interleukin (IL)-1, IL-6, IL-8, IL-10 and (β - defensin (BD)-2, BD-3, BD-4 after the implantation of different biomaterials. Bone developing zones, signs of bone-implant contact and low expression of pro-inflammatory cytokine IL-1, IL-6 and anti-inflammatory cytokine IL-10 in experimental tissue with pure HAp and unburned HAp implants indicate a potential advantage of this material in terms of its biocompatibility over the other materials used in our study.
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    Time-dependent cytokine expression in bone of experimental animals after hydroxyapatite (Hap) implantation
    (2011) Pilmane, M.; Salms, G.; Salma, I.; Skagers, A.; Locs, J.; Loca, D.; Berzina-Cimdina, L.; Institute of Anatomy and Anthropology; Department of Oral and Maxillofacial Surgery and Oral Medicine
    Proinflammatory cytokines mediate bone loss around the implants in patients with peri-implant disease. However, there is no complete data about the expression of cytokines into the bone around the implants. The aim of this work was to investigate the distribution and appearance of inflammatory cytokines and anti-inflammatory proteins in the bone of jaw of experimental rabbits in different time periods after HAp implantation. Material was obtained from 8 rabbits in lower jaw 6 and 8 months after HAp implants were placed. Tissues were processed for immunohistochemical detection of tumor necrosis factor alfa (TNFα), Interleukin 1, 6, 8, 10 (IL-1, IL-6, IL-8, IL-10) and defensin 2. Results demonstrated practically unchanged expression of IL-6 and IL-10 between both - experimental and control side 6 months after implantation, while IL-1 and IL-8 notably increased in control side. IL-1 and IL-10 expression did not change in either the experimental side nor the controle side after 8 months HAP implantation, but IL-6 and IL-8 demonstrated a decrease in the control sites. Only IL-8 was elevated with time in experimental sites, while IL-10 showed individual variations in 2 cases.
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    Распределение интерлейкинов и цитокинов в слизистой оболочке носа и носоглотки при изолированном синдроме постназального затекания
    (2018) Sumeraga, G.; Pilmane, M.; Kise, L.; Rīga Stradiņš University
    The objective of the present study was to elucidate the distribution of interleukins and cytokines in the mucous membranes of the nose and the nasopharynx in the patients presenting with the post-nasal drip syndrome. The study included 20 patients with this pathological condition and 20 volunteers who comprised the control group. The samples of the mucous membranes of the nose and the nasopharynx were examined with the use of the immunohistochemical methods to identify the protein gene product 9.5, interleukin-6, interleukin-10, the tumour necrosis factor-alpha, nuclear factor-kB, and beta-defensin. It was shown that the post-nasal drip syndrome is characterized by the enhanced content of the protein gene product 9.5, interleukin-6, interleukin-10, the tumour necrosis factor-alpha, nuclear factor-kB, and beta-defensin in the mucous membranes of the nose and the nasopharynx.