Browsing by Author "Ērenpreiss, Juris"

Now showing 1 - 8 of 8
  • No Thumbnail Available
    Item
    Anxiety and Depression Symptoms among Infertile Couples Undergoing ART in Latvia : A Cross-Sectional Non-Randomized Single-Centre Study
    (2024-02-09) Popkova, Darja; Bukova-Žideļūna, Aija; Ērenpreiss, Juris; Fodina, Violeta; Institute of Public Health; Rīga Stradiņš University
    Aim: The aim of the study was to evaluate the presence of anxiety and depression among infertile couples undergoing infertility treatment in Latvia. Materials and methods: In this cross-sectional study we compared anxiety and depression symptoms in couples diagnosed with primary infertility (111 women and 55 male), evaluating male and female symptoms separately and together. The level of anxiety and depression were measured using Generalized Anxiety Disorder Scale (GAD-7) and Patient Health Questionnaire-9 (PHQ-9). Results: Upon assessing anxiety and depression level scores mild anxiety is predominantly observed in couples undergoing ART cycles for the first time, accounting for 44.6%, compared to those undergoing it for the 2nd and 3rd time, where the prevalence is equal and stands at 40.7%. Conversely, a noteworthy trend is discerned in patients undergoing ART multiple times, revealing a higher prevalence of moderate and severe anxiety, at 18.6% and 9.3%, respectively. The highest prevalence of depression symptoms is observed in individuals undergoing their second ART cycle and beyond. Particularly noteworthy is the substantial proportion of these patients, with 36.0% experiencing mild and 17.7% experiencing moderate depression symptoms. Conclusions: The experience of undergoing artificial reproductive technology treatment multiple times is associated with heightened prevalence of depression and anxiety among infertile couples. These conclusions highlight the importance of addressing mental health aspects in the context of infertility and ART cycles.
  • No Thumbnail Available
    Item
    Exploring the Potential of Exome Sequencing in Idiopathic Azoospermia : A Genetic Burden and Network Analysis Study
    (2023-08-17) Alkšere, Baiba; Puzuka, Agrita; Lazovska, Marija; Vainselbaum, Ninel Miriam; Vasilonoks, Janis Kristaps; Penka, Elvita; Fodina, Violeta; Ērenpreiss, Juris; Department of Biology and Microbiology; Scientific Laboratory of Molecular Genetics
    The purpose of this study was to investigate the linkage of the association of azoospermia phenotype with genetic alterations, involved in genome instability. Male infertility is a multifactorial pathology, and genetic alterations might be the underlying factors in majority of cases of severe male infertility. The recent emergence of next-generation sequencing offers an opportunity to analyze many genes and their interactions at once, and whole-exome sequencing (WES) together with whole-genome sequencing (WGS) was recently suggested for implementation of diagnosis workup in severe infertility cases. However, the reports on WES in conjunction with burden tests and gene network analysis are scarce or lacking in cases of severe male infertility. WES was performed on 21 nonobstructive azoospermia patients. DNA samples were sequenced using the Twist Comprehensive Exome Panel. Genetic burden test was performed with Testing Rare vAriants using Public Data. Protein interactions were investigated with ConsensusPathDB and Cytoscape. For single nucleotide variants and copy number variations (CNV) analysis, samples were analyzed with the Illumina's BaseSpace Variant Interpreter. Genetic variant burden was found elevated in 1,473 genes out of 30,000 known testis expressed genes. Three hundred and two genes with increased loss-of-function (LoF) variant set were present in more than one sample. Overrepresentation analysis with pathway-based set of genes with high variant burden demonstrated 26 pathways. Overrepresentation analysis with protein complex-based gene sets obtained 14 sets, showing the involvement in cell proliferation and DNA repair. Search Tool for the Retrieval of Interacting Genes/Proteins (STRING) network analysis with Cytoscape identified two clusters: (1) genes, involved in DNA binding/condensation and repair processes and (2) genes with the role in ribosome biosynthesis and gene expression processes. Increased loss of function germline variant burden and sumoylation may have critical significance in spermatogenesis. These parameters may be used for focused diagnosis in nonobstructive azoospermia patients. This may have both general significance for the decreased organism functionality but in particular is critical in spermatogenesis.
  • No Thumbnail Available
    Item
    Jānis Oļģerts Ērenpreiss and His Theory of Carcinogenesis
    (Paula Stradiņa Medicīnas vēstures muzejs. AML Medicīnas vēstures institūts, 2000) Ērenpreisa, Jekaterina; Dālmane, Aina; Ērenpreiss, Juris
  • No Thumbnail Available
    Item
    Mans tēvs akadēmiķis Jānis Ērenpreiss
    (Paula Stradiņa Medicīnas vēstures muzejs. RSU Medicīnas vēstures institūts, 2005) Ērenpreiss, Juris
  • No Thumbnail Available
    Item
    The Price of Human Evolution : Cancer-Testis Antigens, the Decline in Male Fertility and the Increase in Cancer
    (2023-07) Ērenpreisa, Jekaterina; Vainshelbaum, Ninel Miriam; Lazovska, Marija; Kārkliņš, Roberts; Salmina, Kristine; Zayakin, Pawel; Rumnieks, Felikss; Inashkina, Inna; Pjanova, Dace; Ērenpreiss, Juris; Scientific Laboratory of Molecular Genetics
    The increasing frequency of general and particularly male cancer coupled with the reduction in male fertility seen worldwide motivated us to seek a potential evolutionary link between these two phenomena, concerning the reproductive transcriptional modules observed in cancer and the expression of cancer-testis antigens (CTA). The phylostratigraphy analysis of the human genome allowed us to link the early evolutionary origin of cancer via the reproductive life cycles of the unicellulars and early multicellulars, potentially driving soma-germ transition, female meiosis, and the parthenogenesis of polyploid giant cancer cells (PGCCs), with the expansion of the CTA multi-families, very late during their evolution. CTA adaptation was aided by retrovirus domestication in the unstable genomes of mammals, for protecting male fertility in stress conditions, particularly that of humans, as compensation for the energy consumption of a large complex brain which also exploited retrotransposition. We found that the early and late evolutionary branches of human cancer are united by the immunity-proto-placental network, which evolved in the Cambrian and shares stress regulators with the finely-tuned sex determination system. We further propose that social stress and endocrine disruption caused by environmental pollution with organic materials, which alter sex determination in male foetuses and further spermatogenesis in adults, bias the development of PGCC-parthenogenetic cancer by default.
  • No Thumbnail Available
    Item
    Testikulāro spermatozoīdu ekstrakcijas iespējas atbilstoši sēklinieku histoloģiski-morfoloģiskai atradnei pacientiem ar neobstruktīvu azoospermiju
    (Rīga Stradiņš University, 2024) Ābele, Andris; Ērenpreiss, Juris; Faculty of Residency; Rezidentūras studiju fakultāte
    INTRODUCTION. Infertility affects 1 in 6 couples, at least half of the cases are due to male factor infertility. In the past, men with azoospermia were considered sterile, but today they can become biological fathers thanks to medically assisted reproductive technology. Nonobstructive azoospermia (NOA) occurs when spermatogenesis is significantly impaired. Therefore, it is important to use effective surgical methods of sperm extraction and to identify the factors that influence this effectiveness. OBJECTIVES. To investigate the possibility of testicular sperm extraction in patients with NOA according to the histologic-morphologic findings of the testis. METHODS. This study consecutively recruited 54 NOA patients who were treated at the EGV clinic and underwent microsurgical (microTESE) or conventional testicular sperm extraction (cTESE) between 2018 and 2022. The results of demographic data, the presence of cryptorchidism and/or varicocele, changes in karyotype, the presence of Y-chromosome azoospermia factor (AZF), testosterone (T) and follicle-stimulating hormone (FSH) levels, semen analysis, intraoperative testicular sperm extraction rate and testicular biopsy were analyzed. Standard descriptive statistical methods were used to record study characteristics and data analysis using Microsoft Excel 2021 software. RESULTS. Out of 54 NOA patients, 48 (88.9%) underwent a microTESE and 6 (11.1%) underwent a cTESE procedure. Positive testicular sperm extraction was observed in 39 (72.2%) cases. The mean T values in the (+) sperm group is 5.43 (1.31-8.55) ng/mL; in the (-) sperm group 4.26 (2.10-10.50) ng/mL. The mean FSH values are 11.5 (9.5-13.5) U/L in the (+) sperm group and 24.3 (3.7-58.6) U/L in the (-) sperm group, which is a significant difference (p<0.001). For all microTESE/cTESE procedures, sperm extraction in the NOA-I group is 100% (n=25) successful, in the NOA-II group the sperm extraction rate is 85% (n=11), in the NOA-III group 27% (n=3) and in the NOA-SCO group no sperm extraction (n=5) is successful. CONCLUSIONS. MicroTESE is an effective method of surgical testicular sperm extraction in men with NOA, especially in hypospermatogenesis. The histologic-morphologic picture of the testis and the FSH serum level are important prognostic factors for testicular sperm extraction, regardless of age.
  • No Thumbnail Available
    Item
  • No Thumbnail Available
    Item
    Zobārstniecības attīstība Latvijā
    (Paula Stradiņa Medicīnas vēstures muzejs. RSU Medicīnas vēstures institūts, 2002) Ērenpreiss, Juris; Ivanovs, Andrejs