Kalnins, GintsKuka, JanisGrinberga, SolveigaMakrecka-Kuka, MarinaLiepinsh, EdgarsDambrova, MaijaTars, Kaspars2022-01-102022-01-102015-08-28Kalnins, G, Kuka, J, Grinberga, S, Makrecka-Kuka, M, Liepinsh, E, Dambrova, M & Tars, K 2015, 'Structure and function of CutC choline lyase from human microbiota bacterium Klebsiella pneumoniae', Journal of Biological Chemistry, vol. 290, no. 35, pp. 21732-21740. https://doi.org/10.1074/jbc.M115.6704710021-9258https://dspace.rsu.lv/jspui/handle/123456789/7180Publisher Copyright: © 2015 by The American Society for Biochemistry and Molecular Biology, Inc.CutC choline trimethylamine-lyase is an anaerobic bacterial glycyl radical enzyme (GRE) that cleaves choline to produce trimethylamine (TMA) and acetaldehyde. In humans, TMA is produced exclusively by the intestinal microbiota, and its metabolite, trimethylamine oxide, has been associated with a higher risk of cardiovascular diseases. Therefore, information about the three-dimensional structures of TMA-producing enzymes is important for microbiota-targeted drug discovery. We have cloned, expressed, and purified the CutC GRE and the activating enzyme CutD from Klebsiella pneumoniae, a representative of the human microbiota. We have determined the first crystal structures of both the choline-bound and choline-free forms of CutC and have discovered that binding of choline at the ligand-binding site triggers conformational changes in the enzyme structure, a feature that has not been observed for any other characterized GRE.92871393enginfo:eu-repo/semantics/openAccess1.6 Biological sciences3.1 Basic medicine1.1. Scientific article indexed in Web of Science and/or Scopus databaseBiochemistryMolecular BiologyCell BiologySDG 3 - Good Health and Well-being/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article10.1074/jbc.M115.670471http://www.scopus.com/inward/record.url?scp=84940707248&partnerID=8YFLogxK