Pilmane, MaraOzoliņa, LindaĀbola, ZanePetersons, AigarsPopkovs, VjačeslavsDabužinskiene, AnitaVetra, Janis2021-04-282021-04-282011Pilmane, M, Ozoliņa, L, Ābola, Z, Petersons, A, Popkovs, V, Dabužinskiene, A & Vetra, J 2011, 'Growth factors, their receptors, neuropeptide-containing innervation, and matrix metalloproteinases in the proximal and distal ends of the esophagus in children with esophageal atresia', Medicina, vol. 47, no. 8, pp. 453-460. https://doi.org/10.3390/medicina470800661010-660Xhttps://dspace.rsu.lv/jspui/handle/123456789/3969Objective: The pathogenesis of esophageal atresia (EA) remains unknown despite a relatively high incidence of this anomaly in population affecting 1 newborn per 3000 live births. The aim of this study was to examine the relative occurrence of growth factors, their receptors, neuropeptide-containing innervation, and tissue-degradating enzymes - matrix metalloproteinases - in the proximal and distal parts of the esophagus with EA. Materials and Methods: A histopathological study was conducted on 15 patients with EA. Tissues were processed for NGFRp75, PGP 9.5, TGF-β, FGFR, VEGF, EGFR and MMP-2 by means of biotin-streptavidin immunohistochemistry. Results: In the control and EA-affected distal esophageal specimens, numerous and abundant NGFR-containing structures were detected, while in the proximal part of the esophagus, a decrease in their number was observed in patients. PGP 9.5 also marked neuronal structures similarly. TGF-β was found only in occasional cells in the EA-affected esophageal specimens, while control material demonstrated moderate to numerous TGF-β-containing structures. Abundance of FGFR and only occasional appearance of VEGF-positive cells were found in both the control and EA-affected material. A moderate number of connective tissue cells in controls contained EGFR. Compared with controls, the number of MMP-2 expressing cells in the EA-affected tissues was decreased in the proximal esophagus. Conclusions: A decrease in PGP 9.5-containing neuronal structures in the proximal esophagus supports insufficient innervation of this part of the organ in EA. A decrease in MMP-2 positive cells in the esophageal atresia-affected proximal esophagus indicates also a possible decrease of tissue adaptive and regenerative reactions. Low expression of TGF-β and almost the absence of EGFR in the EA-affected specimens may result in disturbances of cell growth, proliferation, and differentiation, indicating a significant role of these substances in morphopathogenesis of EA. FGFR and VEGF seem not to characterize EA pathogenesis.8807006enginfo:eu-repo/semantics/openAccessAtresiaChildrenEsophagusGrowth factorsImmunohistochemistry3.1 Basic medicine3.2 Clinical medicine1.1. Scientific article indexed in Web of Science and/or Scopus databaseGeneral Medicine/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article10.3390/medicina47080066http://www.scopus.com/inward/record.url?scp=84856647840&partnerID=8YFLogxK