Kozlowska, LucynaViegas, SusanaScheepers, Paul T.J.Duca, Radu C.Godderis, LodeMartins, CarlaCiura, KrzesimirJagiello, KarolinaJoão Silva, MariaMahiout, SelmaMārtiņsone, IneseMatisāne, LindaNieuwenhuyse, An vanPuzyn, TomaszSijko-Szpanska, MonikaVerdonck, JelleSantonen, Tiinathe HBM4EU E-waste Study Team2025-01-272025-01-272025-02Kozlowska, L, Viegas, S, Scheepers, P T J, Duca, R C, Godderis, L, Martins, C, Ciura, K, Jagiello, K, João Silva, M, Mahiout, S, Mārtiņsone, I, Matisāne, L, Nieuwenhuyse, A V, Puzyn, T, Sijko-Szpanska, M, Verdonck, J, Santonen, T & the HBM4EU E-waste Study Team 2025, 'HBM4EU E-waste study – An untargeted metabolomics approach to characterize metabolic changes during E-waste recycling', Environment International, vol. 196, 109281. https://doi.org/10.1016/j.envint.2025.1092810160-4120https://dspace.rsu.lv/jspui/handle/123456789/17038Publisher Copyright: © 2025 The Author(s)E-waste contains hazardous chemicals that may be a direct health risk for workers involved in recycling. We conducted an untargeted metabolomics analysis of urine samples collected from male e-waste processing workers to explore metabolic changes associated with chemical exposures in e-waste recycling in Belgium, Finland, Latvia, Luxembourg, the Netherlands, Poland, and Portugal. Questionnaire data and urine samples were obtained from workers involved in the processing of e-waste (sorting, dismantling, shredding, pre-processing, metal, and non-metal processing), as well as from controls with no known occupational exposure. Pre- and post-shift urine samples were collected and analysed using ultrahigh-performance liquid chromatography-mass spectrometry (UPLC-MS). A total of 32 endogenous urinary metabolites were annotated with a Variable Importance in Projection (VIP) above 2, indicating that e-waste recycling is mainly associated with changes in steroid hormone and neurotransmitter metabolism, energy metabolism, bile acid biosynthesis, and inflammation. The highest VIP was observed for dopamine-o-quinone, which is linked to Parkinson's disease. These and other changes in metabolism in workers employed in the processing of e-waste need further verification in targeted studies.128788819enginfo:eu-repo/semantics/openAccessBiomarkersMixture exposureOccupational exposureRecyclingUrine metabolomics3.3 Health sciences1.1. Scientific article indexed in Web of Science and/or Scopus databaseGeneral Environmental ScienceSDG 3 - Good Health and Well-beingSDG 12 - Responsible Consumption and Production/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article10.1016/j.envint.2025.109281http://www.scopus.com/inward/record.url?scp=85215410622&partnerID=8YFLogxK