Semenistaja, SofijaSokolovska, LībaStuders, PēterisKadiša, AndaGroma, ValērijaSkuja, Sandra2024-09-062024-09-062024Semenistaja, S, Sokolovska, L, Studers, P, Kadiša, A, Groma, V & Skuja, S 2024, 'Integrating Articular Cartilage Histopathology with CTX-II and COMP as Synovial Fluid Biomarkers for a Comprehensive Analysis in Osteoarthritis Evaluation', The 3rd International Electronic Conference on Biomolecules, 23/04/24 - 25/04/24 pp. 89. < https://science.rsu.lv/admin/files/88057236/abstract_book.pdf >conferencehttps://dspace.rsu.lv/jspui/handle/123456789/16666Osteoarthritis (OA) is the most common form of chronic joint disease characterized by the loss of cartilage as the primary site of the lesion. Both collagenous and non-collagenous proteins of the extracellular matrix (ECM) are dysregulated by the cellular component damage. Alterations in cartilage composition may manifest through variations in synovial fluid concentrations of C-terminal cross-linked telopeptides of type II collagen (CTX-II) and cartilage oligomeric matrix protein (COMP). This study seeks to decode histological alterations in the cartilage and establish a symbiotic relationship with concentrations of specific ECM proteins within the synovial fluid as potential OA biomarkers. Twenty-five surgically obtained cartilage tissue samples were stained with toluidine blue and analysed using the OARSI grading system under a light microscope both quantitatively and semi-quantitatively. The presence of CTX-II and COMP proteins was measured in the synovial fluid samples using ELISA. The SPSS 28.0 program was used for statistical data analysis. The OARSI score median value was 4.0 (3.0;4.5), attributable to the delamination of the superficial cartilage zone. A greater number of single cells in comparison with cellular clusters was found: 60.00 (45.00;84.00) and 26.00 (22.00;48.00), respectively. Proteoglycan staining was 1.00 (1.00;2.00), attributable to low intensity. A negative correlation between ECM oedema and proteoglycan staining was found (r = −0.445; p = 0.043). The mean levels of CTX-II and COMP in synovial fluid were 1092 pg/mL (SD 537.95) and 1262 ng/mL (SD 339.47), respectively. There was a positive correlation between CTX-II and OA severity (r = 0.305; p = 0.041); the COMP and OA severity correlation was negative (r = −0.286; p = 0.049). The identified positive correlation between OA severity and CTX-II levels in synovial fluid suggests that CTX-II may hold promise as a valuable biomarker for tracking OA progression. This implication opens up possibilities for early detection and targeted interventions in the management of the disease.1111741enginfo:eu-repo/semantics/openAccess3.1 Basic medicine3.2 Clinical medicine3.4. Other publications in conference proceedings (including local)/dk/atira/pure/researchoutput/researchoutputtypes/contributiontoconference/abstracthttps://science.rsu.lv/admin/files/88057236/abstract_book.pdf