Linars, ArtisSilamikelis, IvarsGudra, DitaRoga, AnceFridmanis, Davids2024-04-102024-04-102022-08Linars, A, Silamikelis, I, Gudra, D, Roga, A & Fridmanis, D 2022, 'OverFlap PCR : A reliable approach for generating plasmid DNA libraries containing random sequences without a template bias', PloS one, vol. 17, e0262968. https://doi.org/10.1371/journal.pone.02629681932-6203https://dspace.rsu.lv/jspui/handle/123456789/15427Publisher Copyright: © 2022 Linars et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.Over the decades, practical biotechnology researchers have aimed to improve naturally occurring proteins and create novel ones. It is widely recognized that coupling protein sequence randomization with various effect screening methodologies is one of the most powerful techniques for quickly, efficiently, and purposefully acquiring these desired improvements. Over the years, considerable advancements have been made in this field. However, developing PCR-based or template-guided methodologies has been hampered by resultant template sequence biases. Here, we present a novel whole plasmid amplification-based approach, which we named OverFlap PCR, for randomizing virtually any region of plasmid DNA without introducing a template sequence bias.222372384enginfo:eu-repo/semantics/openAccess2.8 Environmental biotechnology1.6 Biological sciences3.4 Medical biotechnology1.1. Scientific article indexed in Web of Science and/or Scopus databaseGeneral/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article10.1371/journal.pone.0262968http://www.scopus.com/inward/record.url?scp=85135547389&partnerID=8YFLogxK