Please use this identifier to cite or link to this item: 10.1002/jcb.21224
Title: Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues
Authors: Morris, Russell A.
Damon, Brook
Mironov, Vladimir
Kasyanov, Vladimir
Ramamurthi, Anand
Moreno-Rodriguez, Ricardo
Trusk, Thomas
Potts, Jay D.
Goodwin, Richard L.
Davis, Jeff
Hoffman, Stanley
Wen, Xuejun
Sugi, Yukiko
Kern, Christine B.
Mjaatvedt, Corey H.
Turner, Debi K.
Oka, Toru
Conway, Simon J.
Molkentin, Jeffery D.
Forgacs, Gabor
Markwald, Roger R.
Rīga Stradiņš University
Keywords: Collagen;Connective tissues;Fasciclin;Fibrillogenesis;Periostin;1.6 Biological sciences;1.1. Scientific article indexed in Web of Science and/or Scopus database;Biochemistry;Molecular Biology;Cell Biology
Issue Date: 1-Jun-2007
Citation: Morris , R A , Damon , B , Mironov , V , Kasyanov , V , Ramamurthi , A , Moreno-Rodriguez , R , Trusk , T , Potts , J D , Goodwin , R L , Davis , J , Hoffman , S , Wen , X , Sugi , Y , Kern , C B , Mjaatvedt , C H , Turner , D K , Oka , T , Conway , S J , Molkentin , J D , Forgacs , G & Markwald , R R 2007 , ' Periostin regulates collagen fibrillogenesis and the biomechanical properties of connective tissues ' , Journal of Cellular Biochemistry , vol. 101 , no. 3 , pp. 695-711 . https://doi.org/10.1002/jcb.21224
Abstract: Periostin is predominantly expressed in collagen-rich fibrous connective tissues that are subjected to constant mechanical stresses including: heart valves, tendons, perichondrium, cornea, and the periodontal ligament (PDL). Based on these data we hypothesize that periostin can regulate collagen I fibrillogenesis and thereby affect the biomechanical properties of connective tissues. Immunoprecipitation and immunogold transmission electron microscopy experiments demonstrate that periostin is capable of directly interacting with collagen I. To analyze the potential role of periostin in collagen I fibrillogenesis, gene targeted mice were generated. Transmission electron microscopy and morphometric analyses demonstrated reduced collagen fibril diameters in skin dermis of periostin knockout mice, an indication of aberrant collagen I fibrillogenesis. In addition, differential scanning calorimetry (DSC) demonstrated a lower collagen denaturing temperature in periostin knockout mice, reflecting a reduced level of collagen cross-linking. Functional biomechanical properties of periostin null skin specimens and atrioventricular (AV) valve explant experiments provided direct evidence of the role that periostin plays in regulating the viscoelastic properties of connective tissues. Collectively, these data demonstrate for the first time that periostin can regulate collagen I fibrillogenesis and thereby serves as an important mediator of the biomechanical properties of fibrous connective tissues.
DOI: 10.1002/jcb.21224
ISSN: 0730-2312
Appears in Collections:Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure

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