Please use this identifier to cite or link to this item:
10.1073/pnas.1203567109
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DC Field | Value | Language |
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dc.contributor.author | Kanno, Yuri | - |
dc.contributor.author | Hanada, Atsushi | - |
dc.contributor.author | Chiba, Yasutaka | - |
dc.contributor.author | Ichikawa, Takanari | - |
dc.contributor.author | Nakazawa, Miki | - |
dc.contributor.author | Matsui, Minami | - |
dc.contributor.author | Koshiba, Tomokazu | - |
dc.contributor.author | Kamiya, Yuji | - |
dc.contributor.author | Seo, Mitsunori | - |
dc.date.accessioned | 2021-09-08T09:05:01Z | - |
dc.date.available | 2021-09-08T09:05:01Z | - |
dc.date.issued | 2012-06-12 | - |
dc.identifier.citation | Kanno , Y , Hanada , A , Chiba , Y , Ichikawa , T , Nakazawa , M , Matsui , M , Koshiba , T , Kamiya , Y & Seo , M 2012 , ' Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor ' , Proceedings of the National Academy of Sciences of the United States of America , vol. 109 , no. 24 , pp. 9653-9658 . https://doi.org/10.1073/pnas.1203567109 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://dspace.rsu.lv/jspui/handle/123456789/6241 | - |
dc.description.abstract | Movement of the plant hormone abscisic acid (ABA) within plants has been documented; however, the molecular mechanisms that regulate ABA transport are not fully understood. By using a modified yeast two-hybrid system, we screened Arabidopsis cDNAs capable of inducing interactions between the ABA receptor PYR/PYL/ RCAR and PP2C protein phosphatase under low ABA concentrations. By using this approach, we identified four members of the NRT1/PTR family as candidates for ABA importers. Transport assays in yeast and insect cells demonstrated that at least one of the candidates ABA-IMPORTING TRANSPORTER (AIT) 1, which had been characterized as the low-affinity nitrate transporter NRT1.2, mediates cellular ABA uptake. Compared with WT, the ait1/nrt1.2 mutants were less sensitive to exogenously applied ABA during seed germination and/or postgermination growth, whereas overexpression of AIT1/NRT1.2 resulted in ABA hypersensitivity in the same conditions. Interestingly, the inflorescence stems of ait1/ nrt1.2 had a lower surface temperature than those of the WT because of excess water loss from open stomata. We detected promoter activities of AIT1/NRT1.2 around vascular tissues in inflorescence stems, leaves, and roots. These data suggest that the function of AIT1/NRT1.2 as an ABA importer at the site of ABA biosynthesis is important for the regulation of stomatal aperture in inflorescence stems. | en |
dc.format.extent | 6 | - |
dc.format.extent | 901077 | - |
dc.language.iso | eng | - |
dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America | - |
dc.rights | info:eu-repo/semantics/openAccess | - |
dc.subject | Guard cells | - |
dc.subject | Liquid chromatography-tandem mass spectrometry | - |
dc.subject | Water stress | - |
dc.subject | 1.6 Biological sciences | - |
dc.subject | 1.1. Scientific article indexed in Web of Science and/or Scopus database | - |
dc.subject | General | - |
dc.title | Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor | en |
dc.type | /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article | - |
dc.identifier.doi | 10.1073/pnas.1203567109 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=84862174344&partnerID=8YFLogxK | - |
dc.description.status | Peer reviewed | - |
Appears in Collections: | Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure |
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File | Size | Format | |
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Identification_of_an_abscisic_acid_transporter.pdf | 879.96 kB | Adobe PDF | View/Open |
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