Please use this identifier to cite or link to this item: 10.1073/pnas.1203567109
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dc.contributor.authorKanno, Yuri-
dc.contributor.authorHanada, Atsushi-
dc.contributor.authorChiba, Yasutaka-
dc.contributor.authorIchikawa, Takanari-
dc.contributor.authorNakazawa, Miki-
dc.contributor.authorMatsui, Minami-
dc.contributor.authorKoshiba, Tomokazu-
dc.contributor.authorKamiya, Yuji-
dc.contributor.authorSeo, Mitsunori-
dc.date.accessioned2021-09-08T09:05:01Z-
dc.date.available2021-09-08T09:05:01Z-
dc.date.issued2012-06-12-
dc.identifier.citationKanno , Y , Hanada , A , Chiba , Y , Ichikawa , T , Nakazawa , M , Matsui , M , Koshiba , T , Kamiya , Y & Seo , M 2012 , ' Identification of an abscisic acid transporter by functional screening using the receptor complex as a sensor ' , Proceedings of the National Academy of Sciences of the United States of America , vol. 109 , no. 24 , pp. 9653-9658 . https://doi.org/10.1073/pnas.1203567109-
dc.identifier.issn0027-8424-
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/6241-
dc.description.abstractMovement of the plant hormone abscisic acid (ABA) within plants has been documented; however, the molecular mechanisms that regulate ABA transport are not fully understood. By using a modified yeast two-hybrid system, we screened Arabidopsis cDNAs capable of inducing interactions between the ABA receptor PYR/PYL/ RCAR and PP2C protein phosphatase under low ABA concentrations. By using this approach, we identified four members of the NRT1/PTR family as candidates for ABA importers. Transport assays in yeast and insect cells demonstrated that at least one of the candidates ABA-IMPORTING TRANSPORTER (AIT) 1, which had been characterized as the low-affinity nitrate transporter NRT1.2, mediates cellular ABA uptake. Compared with WT, the ait1/nrt1.2 mutants were less sensitive to exogenously applied ABA during seed germination and/or postgermination growth, whereas overexpression of AIT1/NRT1.2 resulted in ABA hypersensitivity in the same conditions. Interestingly, the inflorescence stems of ait1/ nrt1.2 had a lower surface temperature than those of the WT because of excess water loss from open stomata. We detected promoter activities of AIT1/NRT1.2 around vascular tissues in inflorescence stems, leaves, and roots. These data suggest that the function of AIT1/NRT1.2 as an ABA importer at the site of ABA biosynthesis is important for the regulation of stomatal aperture in inflorescence stems.en
dc.format.extent6-
dc.format.extent901077-
dc.language.isoeng-
dc.relation.ispartofProceedings of the National Academy of Sciences of the United States of America-
dc.rightsinfo:eu-repo/semantics/openAccess-
dc.subjectGuard cells-
dc.subjectLiquid chromatography-tandem mass spectrometry-
dc.subjectWater stress-
dc.subject1.6 Biological sciences-
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database-
dc.subjectGeneral-
dc.titleIdentification of an abscisic acid transporter by functional screening using the receptor complex as a sensoren
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article-
dc.identifier.doi10.1073/pnas.1203567109-
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84862174344&partnerID=8YFLogxK-
dc.description.statusPeer reviewed-
Appears in Collections:Research outputs from Pure / Zinātniskās darbības rezultāti no ZDIS Pure

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