Characterization of senescence of culture-expanded human adipose-derived mesenchymal stem cells

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dc.contributor.authorLegzdina, Diana
dc.contributor.authorRomanauska, Anete
dc.contributor.authorNikulshin, Sergey
dc.contributor.authorKozlovska, Tatjana
dc.contributor.authorBerzins, Uldis
dc.date.accessioned2023-01-17T10:40:01Z
dc.date.available2023-01-17T10:40:01Z
dc.date.issued2016
dc.description.abstractBackground and Objectives: Adipose-derived mesenchymal stem cells (ADSCs) are promising candidates in regenerative medicine. The need for in vitro propagation to obtain therapeutic quantities of the cells imposes a risk of impaired functionality due to cellular senescence. The aim of the study was to analyze in vitro senescence of previously cryopreserved human ADSCs subjected to serial passages in cell culture. Methods and Results: ADSC cultures from 8 donors were cultivated until proliferation arrest was reached. A gradual decline of ADSC fitness was observed by altered cell morphology, loss of proliferative, clonogenic and differentiation abilities and increased β-galactosidase expression all of which occurred in a donor-specific manner. Relative telomere length (RTL) analysis revealed that only three tested cultures encountered replicative senescence. The presence of two ADSC subsets with significantly different RTL and cell size was discovered. The heterogeneity of ADSC cultures was supported by the intermittent nature of aging seen in tested samples. Conclusions: We conclude that the onset of in vitro senescence of ADSCs is a strongly donor-specific process which is complicated by the intricate dynamics of cell subsets present in ADSC population. This complexity needs to be carefully considered when elaborating protocols for personalized cellular therapy.en
dc.description.statusPeer reviewed
dc.format.extent13
dc.format.extent721615
dc.identifier.citationLegzdina, D, Romanauska, A, Nikulshin, S, Kozlovska, T & Berzins, U 2016, 'Characterization of senescence of culture-expanded human adipose-derived mesenchymal stem cells', International Journal of Stem Cells, vol. 9, no. 1, pp. 124-136. https://doi.org/10.15283/ijsc.2016.9.1.124
dc.identifier.doi10.15283/ijsc.2016.9.1.124
dc.identifier.issn2005-3606
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/10093
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84969930839&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofInternational Journal of Stem Cells
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectCell aging
dc.subjectHeterogeneity
dc.subjectHuman adipose-derived mesenchymal stem cells
dc.subjectRelative telomere length
dc.subjectSerial passage
dc.subjectSubpopulations
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectDevelopmental Biology
dc.subjectCell Biology
dc.subjectSDG 3 - Good Health and Well-being
dc.titleCharacterization of senescence of culture-expanded human adipose-derived mesenchymal stem cellsen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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