Development of rapid antigen test prototype for detection of SARS-CoV-2 in saliva samples
dc.contributor.author | Kivrane, Agnija | |
dc.contributor.author | Igumnova, Viktorija | |
dc.contributor.author | Liepina, Elza Elizabete | |
dc.contributor.author | Skrastina, Dace | |
dc.contributor.author | Leonciks, Ainars | |
dc.contributor.author | Rudevica, Zanna | |
dc.contributor.author | Kistkins, Svjatoslavs | |
dc.contributor.author | Reinis, Aigars | |
dc.contributor.author | Zilde, Anna | |
dc.contributor.author | Kazaks, Andris | |
dc.contributor.author | Ranka, Renate | |
dc.contributor.institution | Rīga Stradiņš University | |
dc.date.accessioned | 2022-08-16T21:03:50Z | |
dc.date.available | 2022-08-16T21:03:50Z | |
dc.date.issued | 2022-02-25 | |
dc.description | Funding Information: This study was supported by VPP Program, Republic of Latvia Ministry of Education and Science, project No. VPP-COVID-2020/1-0025. Publisher Copyright: © 2022 Taylor and Francis Ltd. All rights reserved. | |
dc.description.abstract | Background: The development of easy-to-perform diagnostic methods is highly important for detecting current coronavirus disease (COVID-19). This pilot study aimed at developing a lateral flow assay (LFA)- based test prototype to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus in saliva samples. Methods: Mice were immunized using the recombinant receptor-binding domain (rRBD) of SARS-CoV-2 virus spike protein. The combinations of the obtained mouse anti-receptor-binding domain (RBD) polyclonal antibodies (PAbs) and several commercial antibodies directed against the SARS-CoV-2 spike protein were used for enzyme-linked immunosorbent assay (ELISA) to select antibody pairs for LFA. The antibody pairs were tested in a LFA format using saliva samples from individuals with early SARS-CoV-2 infection (n = 9). The diagnostic performance of the developed LFA was evaluated using saliva samples from hospitalized COVID-19 patients (n = 111); the median time from the onset of symptoms to sample collection was 10 days (0-24 days, interquartile range (IQR): 7-13). The reverse transcription-polymerase chain reaction (rRT-PCR) was used as a reference method. Results: Based on ELISA and preliminary LFA results, a combination of mouse anti-RBD PAbs (capture antibody) and rabbit anti-spike PAbs (detection antibody) was chosen for clinical analysis of sample. When compared with rRT-PCR results, LFA exhibited 26.5% sensitivity, 58.1% specificity, 50.0% positive prediction value (PPV), 33.3% negative prediction value (NPV), and 38.7% diagnostic accuracy. However, there was a reasonable improvement in assay specificity (85.7%) and PPV (91.7%) when samples were stratified based on the sampling time. Conclusion: The developed LFA assay demonstrated a potential of SARS-CoV-2 detection in saliva samples. Further technical assay improvements should be made to enhance diagnostic performance followed by a validation study in a larger cohort of both asymptomatic and symptomatic patients in the early stage of infection. | en |
dc.description.status | Peer reviewed | |
dc.format.extent | 9 | |
dc.format.extent | 593932 | |
dc.identifier.citation | Kivrane, A, Igumnova, V, Liepina, E E, Skrastina, D, Leonciks, A, Rudevica, Z, Kistkins, S, Reinis, A, Zilde, A, Kazaks, A & Ranka, R 2022, 'Development of rapid antigen test prototype for detection of SARS-CoV-2 in saliva samples', Upsala Journal of Medical Sciences, vol. 127, no. 1, e8207. https://doi.org/10.48101/ujms.v127.8207 | |
dc.identifier.doi | 10.48101/ujms.v127.8207 | |
dc.identifier.issn | 0300-9734 | |
dc.identifier.uri | https://dspace.rsu.lv/jspui/handle/123456789/9450 | |
dc.identifier.url | http://www.scopus.com/inward/record.url?scp=85126399330&partnerID=8YFLogxK | |
dc.language.iso | eng | |
dc.relation.ispartof | Upsala Journal of Medical Sciences | |
dc.rights | info:eu-repo/semantics/openAccess | |
dc.subject | antigen test | |
dc.subject | COVID-19 | |
dc.subject | ELISA | |
dc.subject | Lateral flow assay | |
dc.subject | point-of-care testing | |
dc.subject | polyclonal antibodies | |
dc.subject | 3.1 Basic medicine | |
dc.subject | 3.2 Clinical medicine | |
dc.subject | 1.1. Scientific article indexed in Web of Science and/or Scopus database | |
dc.subject | General Medicine | |
dc.title | Development of rapid antigen test prototype for detection of SARS-CoV-2 in saliva samples | en |
dc.type | /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article |
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