In vitro assessment for antimicrobial activity of Lactobacillus Helveticus and its natural glycopeptides against mastitis causing pathogens in dairy cattle

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dc.contributor.authorGulbe, Gundega
dc.contributor.authorValdovska, Anda
dc.contributor.authorSaulite, Vaira
dc.contributor.authorJermolajevs, Jevgenijs
dc.contributor.institutionInstitute of Microbiology and Virology
dc.date.accessioned2021-04-21T11:15:01Z
dc.date.available2021-04-21T11:15:01Z
dc.date.issued2015
dc.descriptionPublisher Copyright: © Gulbe et al. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.
dc.description.abstractProbiotic lactic acid bacteria have a great potential to control bovine mastitis as well as they are favourable choice to treat many infectious diseases of human. These bacteria are well known as having many properties which make them beneficial to control pathogenic microorganisms. These include, the ability to adhere to cell, the reduction of pathogenic bacteria adherents, the co-aggregation, the production of organic acids, hydrogen peroxide, bacteriocin and etc., to be safe and non-pathogenic, which antagonize pathogenic microorganisms. However, each strain must be well identified and characterized in vitro before using for disease treatment. The aim of the present study was to screen three kind of test suspensions: TS1, TS2 and TS3, which contains probiotic lactic acid bacterium Lactobacillus helveticus or its natural glycopeptides, and other natural immunomodulators, in order to investigate which content were the most effective in inhibiting several mastitis causing bacteria in dairy cattle: coagulase-positive Staphylococcus aureus, coagulase-negative staphylococci S. haemolyticus, S. saprophyticus, S. simulans, S. vitulinus, and Gram-negative bacteria Citrobacter freundii and Serratia liquefaciens. Test suspensions TS1, TS2 and TS3 were adjusted by pH 6.3, then tested in vitro by well diffusion assay to determine their antimicrobial effect against bacteria. Furthermore haemolytic activity of applied test suspensions were determined. In results TS1 (9-13 mm) and TS2 (10-15 mm) showed the inhibition effect on four of eight tested bacterial strains, whereas TS3 did not displayed any antimicrobial effect. TS2 have a greatest antimicrobial activity as they resulted in the largest inhibition zones.en
dc.description.statusPeer reviewed
dc.format.extent6
dc.format.extent528676
dc.identifier.citationGulbe, G, Valdovska, A, Saulite, V & Jermolajevs, J 2015, 'In vitro assessment for antimicrobial activity of Lactobacillus Helveticus and its natural glycopeptides against mastitis causing pathogens in dairy cattle', Open Biotechnology Journal, vol. 9, no. 1, pp. 61-66. https://doi.org/10.2174/1874070701509010061
dc.identifier.doi10.2174/1874070701509010061
dc.identifier.issn1874-0707
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3880
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84950258712&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofOpen Biotechnology Journal
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBeta-glucans
dc.subjectImmunomodulators
dc.subjectLactic acid bacteria
dc.subjectLysozyme
dc.subjectMastitis
dc.subjectProbiotics
dc.subjectWell diffusion assay
dc.subject1.6 Biological sciences
dc.subject3.4 Medical biotechnology
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectBiotechnology
dc.subjectBiophysics
dc.subjectBioengineering
dc.subjectBiochemistry
dc.subjectBiomedical Engineering
dc.subjectSDG 3 - Good Health and Well-being
dc.titleIn vitro assessment for antimicrobial activity of Lactobacillus Helveticus and its natural glycopeptides against mastitis causing pathogens in dairy cattleen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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