An in vitro and in vivo study on the intensity of adhesion and colonization by Staphylococcus epidermidis and Pseudomonas aeruginosa on originally synthesized biomaterials with different chemical composition and modified surfaces and their effect on expression of TNF-α, β-defensin 2 and IL-10 in tissues

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dc.contributor.authorReinis, Aigars
dc.contributor.authorPilmane, Mara
dc.contributor.authorStunda, Agnese
dc.contributor.authorVetra, Janis
dc.contributor.authorKroiča, Juta
dc.contributor.authorRostoka, Dagnija
dc.contributor.authorŠalms, Ģirts
dc.contributor.authorVostroilovs, Antons
dc.contributor.authorDons, Aleksejs
dc.contributor.authorBerziņa-Cimdiņa, Liga
dc.contributor.institutionDepartment of Biology and Microbiology
dc.contributor.institutionInstitute of Anatomy and Anthropology
dc.contributor.institutionRīga Stradiņš University
dc.date.accessioned2021-04-28T10:00:01Z
dc.date.available2021-04-28T10:00:01Z
dc.date.issued2011
dc.description.abstractThe aim of this study was to determine adhesion and colonization of bacteria on the surface of originally synthesized glass-ceramic biomaterials and their effect on inflammation reactions in tissues surrounding the implant. Materials and Methods: Biomaterial discs were contaminated with bacterial suspensions of 10, 102, and 103 colony forming units (CFU)/mL (P. aeruginosa ATCC 27853 and S. epidermidis ATCC 12228), and after 2 hours of cultivation, the intensity of bacterial adhesion was determined. For in vivo tests, the samples were contaminated with 102, and 103 CFU/mL cultivated at 37°C for 2 h to ensure bacterial adhesion. Contaminated biomaterial samples were implanted in the interscapular area of chinchilla rabbits for 2 and 4 weeks. The biomaterials were removed, and using plate count and sonification methods, bacterial colonization on the surface of biomaterials was determined. Moreover, the expression of TNF-α, β-defensin 2, and IL-10 in the surrounding tissues was assessed by using immunohistochemistry methods. Results: P. aeruginosa more intensively colonized biomaterials in the in vivo study as compared with S. epidermidis. Il-10 is a regulatory cytokine, which reduces the intensity of inflammatory cell activity, thus reducing nonspecific resistance of the organism. Conclusions: The expression of TNF-α and IL-10 was not affected by short (2 and 4 weeks) biomaterial implantation. Pronounced cytokine expression in tissues around implanted biomaterials contaminated with P. aeruginosa was observed.en
dc.description.statusPeer reviewed
dc.format.extent6
dc.format.extent1605740
dc.identifier.citationReinis, A, Pilmane, M, Stunda, A, Vetra, J, Kroiča, J, Rostoka, D, Šalms, Ģ, Vostroilovs, A, Dons, A & Berziņa-Cimdiņa, L 2011, 'An in vitro and in vivo study on the intensity of adhesion and colonization by Staphylococcus epidermidis and Pseudomonas aeruginosa on originally synthesized biomaterials with different chemical composition and modified surfaces and their effect on expression of TNF-α, β-defensin 2 and IL-10 in tissues', Medicina, vol. 47, no. 10, pp. 560-565. https://doi.org/10.3390/medicina47100080
dc.identifier.doi10.3390/medicina47100080
dc.identifier.issn1010-660X
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3968
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=84856631200&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofMedicina
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBiomaterials
dc.subjectInterleukin 10
dc.subjectPseudomonas aeruginosa
dc.subjectStaphylococcus epidermidis
dc.subjectTumor necrosis factor α
dc.subjectβ-defensin 2
dc.subject2.5 Materials engineering
dc.subject2.6 Medical engineering
dc.subject3.1 Basic medicine
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral Medicine
dc.titleAn in vitro and in vivo study on the intensity of adhesion and colonization by Staphylococcus epidermidis and Pseudomonas aeruginosa on originally synthesized biomaterials with different chemical composition and modified surfaces and their effect on expression of TNF-α, β-defensin 2 and IL-10 in tissuesen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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