Visualization of keratin with diffuse reflectance and autofluorescence imaging and nonlinear optical microscopy in a rare keratinopathic ichthyosis

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dc.contributor.authorAnker, Pálma
dc.contributor.authorFesűs, Luca
dc.contributor.authorKiss, Norbert
dc.contributor.authorNoll, Judit
dc.contributor.authorBecker, Krisztina
dc.contributor.authorKuroli, Enikő
dc.contributor.authorMayer, Balázs
dc.contributor.authorBozsányi, Szabolcs
dc.contributor.authorLőrincz, Kende
dc.contributor.authorLihacova, Ilze
dc.contributor.authorLihachev, Alexey
dc.contributor.authorLange, Marta
dc.contributor.authorWikonkal, Norbert
dc.contributor.authorMedvecz, Márta
dc.date.accessioned2022-11-15T09:10:01Z
dc.date.available2022-11-15T09:10:01Z
dc.date.issued2021-02-02
dc.descriptionFunding Information: Funding: This work was supported by grants from the National Research, Development and Innovation Office of Hungary—NKFIH (FK_131916, 2019 (Semmelweis University, M.M.)), EFOP-3.6.3-VEKOP-16-2017-00009 (P.A., S.B.); the New National Excellence Program of the Ministry for Innovation and Technology (ÚNKP-20-4-II-SE-7 (N.K.), ÚNKP-19-3-I-SE-78 (L.F.)); and the European Regional Development Fund project “Time-resolved autofluorescence methodology for noninvasive skin cancer diagnostics” (no. 1.1.1.2/16/I/001, agreement no. 1.1.1.2/VIAA/1/16/014 (I.L., A.L., M.L.)). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
dc.description.abstractKeratins are one of the main fluorophores of the skin. Keratinization disorders can lead to alterations in the optical properties of the skin. We set out to investigate a rare form of keratinopathic ichthyosis caused by KRT1 mutation with two different optical imaging methods. We used a newly developed light emitting diode (LED) based device to analyze autofluorescence signal at 405 nm excitation and diffuse reflectance at 526 nm in vivo. Mean autofluorescence intensity of the hyperkeratotic palmar skin was markedly higher in comparison to the healthy control (162.35 vs. 51.14). To further assess the skin status, we examined samples from affected skin areas ex vivo by nonlinear optical microscopy. Two-photon excited fluorescence and second-harmonic generation can visualize epidermal keratin and dermal collagen, respectively. We were able to visualize the structure of the epidermis and other skin changes caused by abnormal keratin formation. Taken together, we were able to show that such imaging modalities are useful for the diagnosis and follow-up of keratinopathic diseases.en
dc.description.statusPeer reviewed
dc.format.extent5490010
dc.identifier.citationAnker, P, Fesűs, L, Kiss, N, Noll, J, Becker, K, Kuroli, E, Mayer, B, Bozsányi, S, Lőrincz, K, Lihacova, I, Lihachev, A, Lange, M, Wikonkal, N & Medvecz, M 2021, 'Visualization of keratin with diffuse reflectance and autofluorescence imaging and nonlinear optical microscopy in a rare keratinopathic ichthyosis', Sensors (Switzerland), vol. 21, no. 4, 1105, pp. 1-11. https://doi.org/10.3390/s21041105
dc.identifier.doi10.3390/s21041105
dc.identifier.issn1424-8220
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/9782
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85100506192&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofSensors (Switzerland)
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectAutofluorescence
dc.subjectDiffuse reflectance
dc.subjectEpidermolytic ichthyosis
dc.subjectHistopathology
dc.subjectHyperkeratosis
dc.subjectKeratin
dc.subjectKRT1
dc.subjectLED
dc.subjectMultiphoton microscopy
dc.subjectNonlinear microscopy
dc.subject1.3 Physical sciences
dc.subject1.4 Chemical sciences
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectAnalytical Chemistry
dc.subjectBiochemistry
dc.subjectAtomic and Molecular Physics, and Optics
dc.subjectInstrumentation
dc.subjectElectrical and Electronic Engineering
dc.titleVisualization of keratin with diffuse reflectance and autofluorescence imaging and nonlinear optical microscopy in a rare keratinopathic ichthyosisen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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