Monocytes/macrophages act as mediators for human herpesvirus-6 infection of thyroid gland in patients with autoimmune thyroiditis

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dc.contributor.authorSokolovska, Liba
dc.contributor.authorSultanova, Alina
dc.contributor.authorČistjakovs, Maksims
dc.contributor.authorCunskis, Egils
dc.contributor.authorMurovska, Modra
dc.contributor.institutionInstitute of Microbiology and Virology
dc.date.accessioned2021-04-12T11:10:03Z
dc.date.available2021-04-12T11:10:03Z
dc.date.issued2019-05-01
dc.descriptionFunding Information: This work was supported by the National Research Programme “Biomedicine for the Public Health” (BIOMEDICINE) Publisher Copyright: © 2019 Liba Sokolovska et al., published by Sciendo 2019. Copyright: Copyright 2019 Elsevier B.V., All rights reserved.
dc.description.abstractThe aim of this study was to investigate the possibility of using monocytes/macrophages as mediators in human herpesvirus-6 (HHV-6) infection of thyroid gland tissues in autoimmune thyroiditis (AIT). Seventy-three AIT patients were enrolled in this study. The control group consisted of 80 blood donors. Monocyte/macrophage isolation for AIT patient samples was performed by adherence. HHV-6 was detected in peripheral blood mononuclear cell (PBMC) DNA samples using nested polymerase chain reaction (nPCR). Gene expression of HHV-6 active infection marker (U79/80) and chemokine receptors (U12, U51) in patient monocyte/macrophage samples and blood donor PBMC samples was detected using reverse-transcription PCR. HHV-6 viral load was detected by using quantitative-PCR technique. The HHV-6 genomic sequence was found significantly more frequently among AIT patient than control group samples. Markers of active infection were found in 8 AIT patient monocyte/macrophage samples (11%) and in none of control group PBMC samples. HHV-6 U51 mRNA expression was detected only in AIT patient samples (2/24 previously positive for HHV-6). Since HHV-6 genomic sequences were found significantly more frequently in AIT patient samples and active infection markers were found in patient monocytes/macrophages, our results suggest that monocytes/macrophages may be used by HHV-6 as mediators for thyroid gland infection.en
dc.description.statusPeer reviewed
dc.format.extent5
dc.format.extent281692
dc.identifier.citationSokolovska, L, Sultanova, A, Čistjakovs, M, Cunskis, E & Murovska, M 2019, 'Monocytes/macrophages act as mediators for human herpesvirus-6 infection of thyroid gland in patients with autoimmune thyroiditis', Proceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences, vol. 73, no. 2, pp. 112-116. https://doi.org/10.2478/prolas-2019-0018
dc.identifier.doi10.2478/prolas-2019-0018
dc.identifier.issn1407-009X
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/3750
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=85065659922&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofProceedings of the Latvian Academy of Sciences, Section B: Natural, Exact, and Applied Sciences
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectautoimmunity
dc.subjectHHV-6
dc.subjectthyroid gland
dc.subject1.6 Biological sciences
dc.subject3.2 Clinical medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectGeneral
dc.titleMonocytes/macrophages act as mediators for human herpesvirus-6 infection of thyroid gland in patients with autoimmune thyroiditisen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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