Quantitative analysis of phenibut in rat brain tissue extracts by liquid chromatography-tandem mass spectrometry

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dc.contributor.authorGrinberga, Solveiga
dc.contributor.authorZvejniece, Liga
dc.contributor.authorLiepinsh, Edgars
dc.contributor.authorDambrova, Maija
dc.contributor.authorPugovics, Osvalds
dc.date.accessioned2021-06-30T10:55:01Z
dc.date.available2021-06-30T10:55:01Z
dc.date.issued2008
dc.description.abstractPhenibut (3-phenyl-4-aminobutyric acid) is a γ-aminobutyric acid mimetic drug, which is used clinically as a mood elevator and tranquilizer. In the present work, a rapid, selective and sensitive liquid chromatography-tandem mass spectrometry method for quantification of phenibut in biological matrices has been developed. The method is based on protein precipitation with acidic acetonitrile followed by isocratic chromatographic separation using acetonitrile-formic acid (0.1% in water; 8:92, v/v) mobile phase on a reversed-phase column. Detection of the analyte was performed by electrospray ionization mass spectrometry in multiple reaction monitoring mode with the precursor-to-product ion transition m/z 180.3 → m/z 117.2. The calibration curve was linear over the concentration range 50-2000 ng/mL. The lower limit of quantification for phenibut in rat brain extracts was 50 ng/mL. Acceptable precision and accuracy were obtained over the whole concentration range. The validated method was successfully applied in a pharmacological study to analyze phenibut concentration in rat brain tissue extract samples.en
dc.description.statusPeer reviewed
dc.format.extent4
dc.format.extent103957
dc.identifier.citationGrinberga, S, Zvejniece, L, Liepinsh, E, Dambrova, M & Pugovics, O 2008, 'Quantitative analysis of phenibut in rat brain tissue extracts by liquid chromatography-tandem mass spectrometry', Biomedical Chromatography, vol. 22, no. 12, pp. 1321-1324. https://doi.org/10.1002/bmc.1059
dc.identifier.doi10.1002/bmc.1059
dc.identifier.issn0269-3879
dc.identifier.urihttps://dspace.rsu.lv/jspui/handle/123456789/5747
dc.identifier.urlhttp://www.scopus.com/inward/record.url?scp=59649107832&partnerID=8YFLogxK
dc.language.isoeng
dc.relation.ispartofBiomedical Chromatography
dc.rightsinfo:eu-repo/semantics/openAccess
dc.subjectBrain tissue extracts
dc.subjectLC-MS-MS
dc.subjectPhenibut
dc.subject3.1 Basic medicine
dc.subject1.1. Scientific article indexed in Web of Science and/or Scopus database
dc.subjectAnalytical Chemistry
dc.subjectBiochemistry
dc.subjectMolecular Biology
dc.subjectPharmacology
dc.subjectDrug Discovery
dc.subjectClinical Biochemistry
dc.titleQuantitative analysis of phenibut in rat brain tissue extracts by liquid chromatography-tandem mass spectrometryen
dc.type/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article

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